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11.

Improved rat spinal cord injury model using spinal cord compression by percutaneous method

Wook-Hun Chung, Jae-Hoon Lee, Dai-Jung Chung, Wo-Jong Yang, A-Jin Lee, Chi-Bong Choi, Hwa-Seok Chang, Dae-Hyun Kim, Hyo Jin Chun 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 13 329-335 (7 pages)

Wook-Hun Chung, Jae-Hoon Lee, Dai-Jung Chung, Wo-Jong Yang, A-Jin Lee, Chi-Bong Choi, Hwa-Seok Chang, Dae-Hyun Kim, Hyo Jin Chun 저
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- 3,000원
Here, percutaneous spinal cord injury (SCI) methods using a balloon catheter in adult rats are described. A balloon catheter was inserted into the epidural space through the lumbosacral junction and then inflated between T9-T10 for 10min under fluoroscopic guidance. Animals were divided into three groups with respect to inflation volume: 20 μL (n = 18), 50 μL (n = 18) and control (Fogarty catheter inserted but not inflated; n = 10). Neurological assessments were then made based on BBB score,...
12.

A modified method for inducing periodontitis in dogs using a silk-wire twisted ligature

Se Eun Kim, Eui Ri Lee, Yesran Lee, Manbok Jeong, Young Woo Park, Jae Sang Ahn, Jeong Taek Ahn, Kangmoon Seo* 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 11 193-197 (5 pages)

Se Eun Kim, Eui Ri Lee, Yesran Lee, Manbok Jeong, Young Woo Park, Jae Sang Ahn, Jeong Taek Ahn, Kangmoon Seo* 저
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- 1,500원
parameters including the plaque index, gingival index, clinical attachment level, and bleeding on probing were significantly exacerbated in the SWL group compared to the other groups (p ＜ 0.05). Radiographic evaluation demonstrated that alveolar bone levels were significantly lower in the SWL group than the other groups on day 60 (p ＜ 0.05). These results suggested that experimental periodontitis induced by SWL could be an effective method for investigating periodontitis in canine models.
13.

A Novel Nicotinamide Adenine Dinucleotide Correction Method for Mitochondrial Ca2+ Measurement with FURA-2-FF in Single Permeabilized Ventricular Myocytes of Rat

JeongHoonLee, JeongMiHa, ChaeHunLeem 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages

대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2015, Vol.19 No.4 10 373-382 (10 pages)

JeongHoonLee, JeongMiHa, ChaeHunLeem 저
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- 3,000원
a novel online method that corrected NADH and Fura-2-FF interference. We simultaneously measured multiple parameters, including NADH, [Ca2+], and pH/mitochondrial membrane potential; Fura-2-FF for mitochondrial [Ca2+] and TMRE for Ψm or carboxy-SNARF-1 for pH were used. With this novel method, we found that the resting mitochondrial [Ca2+] concentration was 1.03 μM. This 1 μM cytosolic Ca2+ could theoretically increase to more than 100 mM in mitochondria. However, the mitochondrial [Ca2+]...
14.

Development of a Multiplex PCR Method to Detect Fungal Pathogens for Quarantine on Exported Cacti

한국식물병리학회 The Plant Pathology Journal 5 Pages

한국식물병리학회 The Plant Pathology Journal 2016, 32권 1호 7 53-57 (5 pages)

저
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- 1,500원
Major diseases in grafted cacti have been reported and Fusarium oxysporum, Bipolaris cactivora, Phytophthora spp. and Collectotrichum spp. are known as causal pathogens. These pathogens can lead to plant death after infection. Therefore, some European countries have quarantined imported cacti that are infected with specific fungal pathogens. Consequently, we developed PCR detection methods to identify four quarantined fungal pathogens and reduce export rejection rates of Korean grafted cacti....
15.

Development of a Rapid Detection Method for Potato virus X by Reverse Transcription Loop-Mediated Isothermal Amplification

한국식물병리학회 The Plant Pathology Journal 7 Pages

한국식물병리학회 The Plant Pathology Journal 2015, 31권 3호 3 219-225 (7 pages)

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- 3,000원
The primary step for efficient control of viral diseases is the development of simple, rapid, and sensitive virus detection. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been used to detect viral RNA molecules because of its simplicity and high sensitivity for a number of viruses. RT-LAMP for the detection of Potato virus X (PVX) was developed and compared with conventional reverse transcription polymerase chain reaction (RT-PCR) to demonstrate its advantages over...
16.

Simple Method to Produce in vitro Pyrenophora tritici-repentis Teleomorph

한국식물병리학회 The Plant Pathology Journal 3 Pages

한국식물병리학회 The Plant Pathology Journal 2014, 30권 4호 12 437-439 (3 pages)

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- 1,500원
A fungus Pyrenophora tritici-repentis induces tan spot of wheat which is a foliar disease that causes yield loss to wheat crops worldwide. In this study, a new, simple and non-costly technique was performed to produce the sexual stage of this fungus in culture, within 9 weeks using wheat straw. This protocol will be helpful to researchers studying the biology of sexual stage development, disease epidemiology and genetics of this fungus.
17.

A Rapid and Efficient Method for Construction of an Infectious Clone of Tomato yellow leaf curl virus

한국식물병리학회 The Plant Pathology Journal 6 Pages

한국식물병리학회 The Plant Pathology Journal 2014, 30권 3호 10 310-315 (6 pages)

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- 3,000원
efficient evaluation of resistance to TYLCV. Here, we present the rolling circle amplification (RCA) method, which employs a bacteriophage using phi-29 DNA polymerase for construction of infectious TYLCV clones. The RCA method is simple, does not require sequence information for cloning, and is less expensive and time consuming than conventional PCR based-methods. Furthermore, RCA-based construction of an infectious clone can be very useful to other emerging and unknown geminiviruses in Korea.
18.

A Simple Method for the Assessment of Fusarium Head Blight Resistance in Korean Wheat Seedlings Inoculated with Fusarium graminearum

한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2014, 30권 1호 3 25-32 (8 pages)

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- 3,000원
P<0.05). Results from two other seedling inoculation methods, spraying and pin-point inoculation, were not correlated with adult FHB resistance. Single linear correlation was not significant between seed germination assays (soaking and soak-dry) and FHB resistance (Type I and Type II), respectively. These results showed that clip-dipping inoculation method using F. graminearum may offer a real possibility of simple, rapid, and reliable for the early screening of FHB resistance in wheat.
19.

Note : Open Access ; Arabidopsis PYL8 Plays an Important Role for ABA Signaling and Drought Stress Responses

한국식물병리학회 The Plant Pathology Journal 6 Pages

한국식물병리학회 The Plant Pathology Journal 2013, 29권 4호 16 471-476 (6 pages)

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- 3,000원
Plants are frequently exposed to numerous environmental stresses such as dehydration and high salinity, and have developed elaborate mechanisms to counteract the deleterious effects of stress. The phytohormone abscisic acid (ABA) plays a critical role as an integrator of plant responses to water-limited condition to activate ABA signal transduction pathway. Although perception of ABA has been suggested to be important, the function of each ABA receptor remains elusive in dehydration condition....
20.

Note : Open Access ; Occurrence of Stolbur Phytoplasma Disease in Spreading Type Petunia hybrida Cultivars in Korea

한국식물병리학회 The Plant Pathology Journal 6 Pages

한국식물병리학회 The Plant Pathology Journal 2013, 29권 4호 15 465-470 (6 pages)

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- 3,000원
In January 2012, spreading type petunia cv. Wave Pink plants showing an abnormal growth habit of sprouting unusual multiple plantlets from the lateral buds were collected from a greenhouse in Gwacheon, Gyeonggi Province, Korea. The presence of phytoplasma was investigated using PCR with the primer pairs P1/P6, and R16F1/R1 for nested-PCR. In the nested PCR, 1,096 bp PCR products were obtained, and through sequencing 12 Pet-Stol isolates were identified. Comparison of the nucleotide sequences of...

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