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Duplex nested reverse transcriptase polymerase chain reaction for simultaneous detection of type 2 porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 from tissue samples
Hye Kwon Kim, Kwang Soo Lyoo, Thi My Le Huynh, Hyoung Joon Moon, Van Giap Nguyen, Bong Kyun Park 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 16 253-256 (4 pages)
duplex nested reverse transcriptase polymerase chain reaction (RT-PCR) method that targets the genomic RNA of type 2 PRRSV and the mRNA of PCV2 in infected tissues. The method amplified discriminative bands of 347 bp and 265 bp specific for type 2 PRRSV and PCV2, respectively. The limits of detection of the duplex nested RT-PCR were 10 TCID50/mL for type 2 PRRSV and 10 infected cells/mL for PCV2. The kappa statistic, which measures agreement between methods, was 0.867, indicating a good level of... -
Detection of Streptococcus dysgalactiae subsp. equisimilis in equine nasopharyngeal swabs by PCR
Silvia Preziuso, *, Fulvio Laus, Aurora Romero Tejeda, Carlo Valente, Vincenzo Cuteri2 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 1호 10 67-72 (6 pages)
been isolated from cases of strangles-like disease. Rapid and sensitive diagnostic techniques could enable epidemiological studies and effective investigation of outbreaks involving these bacteria. In this study, PCR protocols previously described in cattle and in humans to detect the species S. dysgalactiae and the subspecies equisimilis were evaluated to detect specific sequences in equine samples. For this purpose, 99 monolateral nasal swabs were collected from horses from stud farms with a... -
Development of a Multiplex PCR Method to Detect Fungal Pathogens for Quarantine on Exported Cacti
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 1호 7 53-57 (5 pages)
spp. are known as causal pathogens. These pathogens can lead to plant death after infection. Therefore, some European countries have quarantined imported cacti that are infected with specific fungal pathogens. Consequently, we developed PCR detection methods to identify four quarantined fungal pathogens and reduce export rejection rates of Korean grafted cacti. The pathogen specific primer sets F.oF-F.oR, B.CF-B.CR, P.nF-P.nR, and P.cF-P.CR were tested for F. oxysporum, B. cactivora, P.... -
Development and Practical Use of RT-PCR for Seed-transmitted Prune dwarf virus in Quarantine
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2014, 30권 2호 8 178-182 (5 pages)
In this study, two RT-PCR primer sets, which can promptly and specifically diagnose plant quarantine seed-transmitted PDV, were developed; and nested PCR primers, where products amplify 739 and 673 nucleotides (nt), and an nested PCR-product, 305 nt, can be obtained as these products are amplified again, were developed. Also, a modified-positive control plasmid was developed, where the restriction enzyme XhoI, which can identify the contamination of samples from the control, was inserted. The... -
Research Notes : Multiplex PCR Assay for Simultaneous Detection of Korean Quarantine Phytoplasmas
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 4호 10 367-371 (5 pages)
Multiplex PCR assays were developed for the simultaneous detection of ten important Korean quarantine phytoplasmas. The species-specific primers were designed based on ribosomal protein, putative preprotein translocase Y, immunodominant protein, elongation factor TU, chaperonin protein and the 16S rRNA genes of ``Candidatus (Ca.) Phytoplasma`` species. Three main primer sets were prepared from ten designed primer pairs to limit nonspecific amplification as much as possible. The multiplex PCR... -
Development of a Multiplex RT-PCR for the Simultaneous Detection of Three Viruses in Cherry Plants
Chung Youl Park, Jeong-ran Park, Geun-sik Lee, Seung-In Yi, Byeong Hoon Kim, Jung Sik Eom, Som Gyeol Lee, Hong-sup Kim 한국식물병리학회 식물병연구 4 Pages
한국식물병리학회 식물병연구 2018, 24권 3호 7 233-236 (4 pages)
A multiplex RT-PCR (mRT-PCR) assay was developed for the detection of the recently reported viruses, Cherry virus A (CVA), Little cherry virus 1 (LChV-1), and Little cherry virus 2 (LChV-2), in cherry plants in Korea. Eight sets of primers were designed for each virus and their specificity was tested by using various combinations of mixed primer sets. From the designed primer sets, one combination was selected and further evaluated to estimate the optimum temperature and detection limits of the...
