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191.

Red blood cell elution time of strains of Newcastle disease virus

M. C.O Ezeibe, E. T. Ndip 대한수의학회 Journal of Veterinary Science 2 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 4호 4 287-288 (2 pages)

M. C.O Ezeibe, E. T. Ndip 저
...
- 무료
Elution time of velogenic, mesogenic and lentogenic strains of Newcastle disease virus was determined. The differences in their elution time were also calculated. Four samples, each of a velogenic strain (VGF2), a mesogenic strain (Komarov) and a lentogenic strain (LaSota) were used for hemagglutination test with 0.6% chicken red blood cells. The time it took for wells of the end hemagglutination points (highest dilution that gave agglutination) to elute was recorded as elution time for each...
192.

Apoptosis in Vero cells infected with Akabane, Aino and Chuzan virus

Seong In Lim, Chang Hee Kweon, Dong Kun Yang, Dong Seob Tark, Jun Hun Kweon 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 3호 14 251-254 (4 pages)

Seong In Lim, Chang Hee Kweon, Dong Kun Yang, Dong Seob Tark, Jun Hun Kweon 저
...
- 1,500원
on viruses. Although the treatment of cycloheximide blocked apoptosis in Vero cells infected with three viruses, actinomycin D did not prevent DNA oligomerization, thus indicating that de novo viral protein synthesis is critical for viral apoptosis. In addition, the activation of caspase-3 was also detected in Vero cells by indirect fluorescent assay. From the present results, it is of future interest whether apoptotic characteristics of these viruses are related to pathogenecity in vivo.
193.

Immunogenicity of baculovirus expressed recombinant proteins of Japanese encephalitis virus in mice

Dong-Kun Yang, Chang-Hee Kweon, Byoung-Han Kim, Seong-In Lim, Jun-Hun Kwon, Seong-Hee Kim, Jae-Young Song, Hong-Ryul Han 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 6 125-133 (9 pages)

Dong-Kun Yang, Chang-Hee Kweon, Byoung-Han Kim, Seong-In Lim, Jun-Hun Kwon, Seong-Hee Kim, Jae-Young Song, Hong-Ryul Han 저
...
- 3,000원
Genes encoding for the premembrane and envelope (prME), envelope (E) and nonstructural protein (NS1) of Japanese encephalitis virus (JEV) were cloned. Each protein was expressed in baculovirus expression system. Of the three proteins expressed in baculovirus system, only prME had hemagglutination activity. The prME (72 and 54 kDa), E (54 kDa) and NS1 (46 kDa) proteins could be detected by Western blotting in the recombinant virus infected cells. Immunogenicity of the recombinant proteins...
194.

Human embryonic stem cells and therapeutic cloning

Woo Suk Hwang, Byeong Chun Lee, Chang Kyu Lee, Sung Keun Kang 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 1 87-96 (10 pages)

Woo Suk Hwang, Byeong Chun Lee, Chang Kyu Lee, Sung Keun Kang 저
...
- 3,000원
The remarkable potential of embryonic stem (ES) cells is their ability to develop into many different cell types. ES cells make it possible to treat patients by transplanting specialized healthy cells derived from them to repair damaged and diseased cells or tissues, known as “stem cell therapy”. However, the issue of immunocompatibility is one of considerable significance in ES cell transplantation. One approach to overcome transplant rejection of human ES (hES) cells is to derive hES cells...
195.

Induction of castration by immunization of male dogs with recombinant gonadotropin-releasing hormone (GnRH)-canine distemper virus (CDV) T helper cell epitope p35

Mi-Jeong Jung, Young-Chan Moon, Ik-Hyun Cho, Jung-Yong Yeh, Sun-Eui Kim, Wha-Seok Chang, Seung-Young Park, Chang-Seon Song, Hwi- 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 1호 3 21-24 (4 pages)

Mi-Jeong Jung, Young-Chan Moon, Ik-Hyun Cho, Jung-Yong Yeh, Sun-Eui Kim, Wha-Seok Chang, Seung-Young Park, Chang-Seon Song, Hwi- 저
...
- 1,500원
protein and goat rotavirus VP6 protein were produced in E. coli. When these fusion proteins were injected to male dogs which were previously immunized with CDV vaccine, the fusion protein of GnRH-CDV Th cell epitope p35 induced much higher antibody than that of GnRH-rotavirus VP6 protein or GnRH alone. The degeneration of spermatogenesis was also verified in the male dogs immunized with the fusion protein of GnRH-CDV Th cell epitope p35. These results indicate that canine GnRH conjugated to CDV...
196.

Characterization and localization of the unique Marek's disease virus type 2 ORF873 gene product

Hyung-Kwan Jang 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 3호 4 207-213 (7 pages)

Hyung-Kwan Jang 저
...
- 3,000원
2 (MDV2)-unique gene, ORF873, under the polyhedrin promoter was constructed and designated rAcORF873. Polyclonal and monoclonal antibodies, which recognize the recombinant MDV2 ORF873 protein in Spodoptera frugiperda clone 9 (Sf9) cells infected with rAcORF873, were prepared by immunizing mice with a recombinant fusion protein expressed in Escherichia coli. Immunoblot analyses with the antibodies revealed a major protein band with a molecular mass of 108-kDa in both MDV2-infected chick embryo...
197.

Molecular characterization of full-length genome of Japanese encephalitis virus (KV1899) isolated from pigs in Korea

Dong Kun Yang, Byoung Han Kim, Chang Hee Kweon, Jun Hun Kwon, Seong In Lim, Hong Ryul Han 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 3호 3 197-205 (9 pages)

Dong Kun Yang, Byoung Han Kim, Chang Hee Kweon, Jun Hun Kwon, Seong In Lim, Hong Ryul Han 저
...
- 3,000원
We have determined the complete nucleotide and deduced amino acid sequences of the Japanese encephalitis virus (JEV) strain KV1899, isolated from a fattening pig in Korea. In comparison with 22 fully sequenced JEV genomes currently available, we found that the 10,963- nucleotide RNA genome of KV1899 has a 13-nucelotide deletion in the 3' non-translated variable region and 53 unique nucleotide sequences including 3' non-translated region (NTR). Its single open reading frame has a total of 28...
198.

Expression of Recombinant Porcine Interleukin-2 and Application of Its Antibody to Immunoassays

In-Soo Choi and Han-Sang Yoo 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 10 207-212 (6 pages)

In-Soo Choi and Han-Sang Yoo 저
...
- 3,000원
Interleukin-2 plays an important role in T lymphocyte proliferation and immune response regulations. In this study, porcine IL-2 cDNA was cloned from peripheral blood mononuclear cells, and recombinant porcine IL-2 (rpIL-2) was expressed in Escherichia coli. The size of rpIL-2 without signal peptides was about 15 kDa when determined by SDS-PAGE and Western blotting analysis. Anti-rpIL-2 antibody was produced from mice immunized with the purified rpIL-2, and its specificity was examined by...
199.

SR144528 as Inverse Agonist of CB2 Cannabinoid Receptor

Man-Hee Rhee and Sang-Keun Kim 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 6 179-184 (6 pages)

Man-Hee Rhee and Sang-Keun Kim 저
...
- 3,000원
G protein- coupled receptors can signal without agonist stimulation (constitutive receptors). Inverse agonists have been shown to inhibit the activity of such constitutive G protein-coupled receptor signaling. Agonist activation of the G(i/o)-coupled peripheral cannabinoid receptor CB2 normally inhibits adenylyl cyclase type V and stimulates adenylyl cyclase type II. Using transfected COS cells, we show here that application of SR144528, an inverse agonist of CB2, leads to a reverse action...
200.

Immunohistochemistry of Voltage-Gated Calcium Channel α1B Subunit in Mouse Cerebellum

Seung-Hyuk Chung, Kyung-Youl Lee, Ki-Hyung Kim, Chul-Tae Kim, Nam-Seob Lee, Kazuhiko Sawada, Hiromi Haga, Byung-Chul Lee, Yoshih 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 5 175-178 (4 pages)

Seung-Hyuk Chung, Kyung-Youl Lee, Ki-Hyung Kim, Chul-Tae Kim, Nam-Seob Lee, Kazuhiko Sawada, Hiromi Haga, Byung-Chul Lee, Yoshih 저
...
- 1,500원
Secretion of neurotransmitters is initiated by voltage-gated calcium influx through presynaptic, voltage- gated N-type calcium channels. However, little is known about their cellular distribution in the mouse cerebellum. In the cerebellum, alpha1B immunoreactivity is found mainly on the cell bodies of all Purkinje cells. In addition, the immunoreactivity was detected on a subset of Purkinje cell dendrites, clustered to form a parasagittal array of bands. In the anterior lobe vermis,...

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