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PCR-based detection of genes encoding virulence determinants in Staphylococcus aureus from bovine subclinical mastitis cases
Dewanand Rajaram Kalorey, Yuvaraj Shanmugam, Nitin Vasantrao Kurkure, Kapil Kamalakarrao Chousalkar, Sukhadeo Baliram Barbuddhe 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 8 151-154 (4 pages)
The present study was carried out to genotypically characterize Staphylococcus aureus (S. aureus) isolated from bovine mastitis cases. A total of 37 strains of S. aureus were isolated during processing of 552 milk samples from 140 cows. The S. aureus strains were characterized phenotypically, and were further characterized genotypically by polymerase chain reaction using oligonucleotide primers that amplified genes encoding coagulase (coa), clumping factor (clfA), thermonuclease (nuc),... -
Epigallocatechin-3 gallate prevents cardiac hypertrophy induced by pressure overload in rats
Jia Hao, Chan-Hyung Kim, Tae-Sun Ha, Hee-Yul Ahn 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 4 121-129 (9 pages)
Pressure overload diseases, such as valvular stenosis and systemic hypertension, manifest morphologically in patients as cardiac concentric hypertrophy. Prevention of cardiac remodeling due to increased pressure overload is important to reduce morbidity and mortality. Epigallocatechin-3 gallate (EGCG) is a major bioactive polyphenol present in green tea which has been found to be a nitric oxide-mediated vasorelaxant and to be cardioprotective in myocardial ischemia-reperfusion injury. Therefore,... -
The micronucleus frequency in cytokinesis-blocked lymphocytes of cattle in the vicinity of a nuclear power plant
Hae-June Lee, Chang-Mo Kang, Se-Ra Kim, Jong-Choon Kim, Chun-Sik Bae, Ki-Seok Oh, Sung-Kee Jo, Tae-Hwan Kim, Jong-Sik Jang, Sung 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 3 117-120 (4 pages)
Cytogenetic and hematological analyses were performed on the peripheral blood lymphocytes (PBLs) obtained from Korean native cattle bred in the vicinity of three nuclear power plants (Wolsong, Uljin and Yeonggwang) and in a control area. The micronucleus (MN) rates for the cattle from the Wolsong, Uljin and Yeonggwang nuclear power plants and for the control area were 9.87 ± 2.64, 8.90 ± 3.84, 9.20 ± 3.68 and 9.60 ± 3.91 per 1,000 cytokinesis-blocked lymphocytes, respectively. The apparent... -
Immunohistochemical expression of h-telomerase reverse transcriptase in canine and feline meningiomas
Luciana Mandrioli, Serena Panarese, Alessandro Cesari, Maria Teresa Mandara, Paolo Stefano Marcato, Giuliano Bettini 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 2 111-115 (5 pages)
Telomere length maintenance is regarded as a fundamental step in tumorigenesis, as most human brain tumors, including meningiomas, stabilize the ends of their chromosomes using telomerase. This investigation represents an introduction to telomerase expression in canine and feline meningiomas. Twenty-five archived cases (14 dogs and 11 cats) were immunohistochemically tested for human-telomerase reverse transcriptase (h- TERT), scored, and quantified; furthermore, mitoses were counted on sections... -
Electro-acupuncture and Chinese herbs for treatment of cervical intervertebral disk disease in a dog
Ayne Murata Hayashi, Julia Maria Matera, Tatiana Soares da Silva, Ana Carolina Brandao de Campos Fonseca Pinto, Silvia Renata Ga 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 14 95-98 (4 pages)
A non-ambulatory dog with tetraparesis following a pain episode that had evolved over 2 months was submitted for medical treatment and diagnosed with intervertebral disk disease at C3-C4 and dorsal extradural compression at C1-C2 and C3-C4 using myelography and computed tomography. The dog experienced ambulation recovery after 15 days of treatment with only electroacupuncture and Chinese herbal medicine, with marked improvement occurring after only 10 treatments. Six months of followup... -
Birth of puppies after intrauterine and intratubal insemination with frozen-thawed canine semen
Hye Jin Kim, Hyun Ju Oh, Goo Jang, Min Kyu Kim 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 11 75-80 (6 pages)
The present study was performed to assess the fertility of frozen-thawed dog semen prepared by freezing with 6% glycerol and thawing at 70ºC for 8 sec, and to evaluate the least number of post-thaw spermatozoa necessary to achieve pregnancy by intrauterine or intratubal artificial insemination. It was found that the pregnancy rate of intrauterine artificial insemination was 100% using 6% glycerol buffer and thawing at 70ºC for 8 sec with 5 × 107 spermatozoa. Even though the pregnancy rate... -
Development of a monoclonal antibody-based co-agglutination test to detect enterotoxigenic Escherichia coli isolated from diarrheic neonatal calves
Brajesh C. Varshney, N.M. Ponnanna, Pranati A. Sarkar, Pragna Rehman, Jigar H. Shah 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 9 57-64 (8 pages)
Escherichia coli (E. coli) strains were collected from young diarrheic calves in farms and field. Strains that expressed the K99 (F5) antigen were identified by agglutination tests using reference antibodies to K99 antigen and electron microscopy. The K99 antigen from a selected field strain (SAR-14) was heat-extracted and fractionated on a Sepharose CL-4B column. Further purification was carried out by sodium deoxycholate treatment and/or ion-exchange chromatography. Monoclonal antibodies to... -
Sero-survey on Aino, Akabane, Chuzan, bovine ephemeral fever and Japanese encephalitis virus of cattle and swine in Korea
Seong In Lim, Chang Hee Kweon, Dong Seob Tark, Seong Hee Kim, Dong Kun Yang 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 7 45-49 (5 pages)
Vector-borne arboviruses produce mild to severe symptoms in domestic animals. Bovine ephemeral fever (BEF), Akabane, Aino, and Chuzan virus have been primarily attributed to reproductive disorders or febrile diseases in cattle, and Japanese encephalitis virus (JEV) is mainly associated with reproductive failures in swine. We investigated antibody titers from domestic swine against four bovine arboviruses (BEF, Akabane, Aino, and Chuzan virus) and from cattle against JEV in Korea. While the... -
Effects of DDA, CpG-ODN, and plasmid-encoded chicken IFN-γ on protective immunity by a DNA vaccine against IBDV in chickens
Ha Jung Roh, Haan Woo Sung, Hyuk Moo Kwon 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 10 361-368 (8 pages)
This study examined the adjuvant effects of dimethyl dioctadecyl ammonium bromide (DDA), CpG oligodeoxynucleotides (CpG-ODN), and chicken interferon-γ (ChIFN-γ) on a DNA vaccine (pcDNA-VP243) against the infectious bursal disease virus (IBDV). A plasmid encoding chicken IFN-a was constructed. Twice at 2-week intervals, twoweek- old chickens were injected intramuscularly and intraperitoneally with either a DNA vaccine alone or a DNA vaccine together with the respective adjuvants. On week 2... -
The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates
Tae Jung Kim, Jae Il Lee 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 3호 11 277-280 (4 pages)
Using three reference strains of Brachyspira hyodysenteriae (B204, B234, B169), one B. pilosicoli (P43/6/78), one B. murdochii (56-150), one B. intermedia (PWS/A), one B. innocens (B256) and ten Korean isolates, PCR-RFLP analysis of DNA encoding 23S rRNA was performed to establish a rapid and accurate method for characterizing porcine intestinal spirochetes. Consequently, B. hyodysenteriae and B. pilosicoli revealed different restriction patterns; however, the other three species shared the same...


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