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Effect of Reproductive Status on In Vitro Developmental Competence of Bovine Oocytes
Kazim R. Chohan, Alan G. Hunter 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 1호 10 67-72 (6 pages)
of bovine oocytes recovered from ovaries during the follicular, metestrus and diestrus stages of the estrous cycle and at anestrus and pregnancy after maturation in a serum free culture medium. Cumulus oocyte complexes (COCs) collected from ovaries at different reproductive statuses were matured in medium 199 supplemented with 10 g/ml FSH, 10 g/ml LH, 1.5 g/m estradiol, 75 g/ml streptomycin, 100 IU/ml penicillin and 10 mM HEPES. COCs were incubated in 200 microl droplets of maturation medium 199... -
Development and Evaluation of a New Apparatus for Continuous Perfusion of Isolated Perfused Pig Heart
Mi-Young An, Emmanuelle P. Canel, In-Ho Jang, Didier Revel, Theresa W. Fossum, Nam-Sik Chungand Marc F. Janier 대한수의학회 Journal of Veterinary Science 14 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 12 219-232 (14 pages)
the control hearts fibrillated. Two control hearts stopped beating, and only one of the two survived with the help of pacemaker.The coronary systolic, diastolic, and mean pressures were more stable with low variation in the experimental hearts than the cold ischemic control hearts. The experimental hearts consumed more oxygen than the control hearts, indicating more cardiac output. According to these results, the continuous normothermic perfusion method by the new cannula, even though with a... -
Prevalence and Clinical Characterization of Gastric Helicobacter Species Infection of Dogs and Cats in Korea
Cheol-Yong Hwang, Hong-Ryul Hanand Hwa-Young Youn 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 2호 10 123-134 (12 pages)
scores of fundic tissues from clinically abnormal infected dogs were similar to that from noninfected dogs and evidence of upregulation of IL-1beta, IL-8, and TNF-alpha mRNA was not detected in gastric fundic tissues from clinically abnormal infected dogs. This study suggested that Helicobacter spp. infection in domestic dogs including private owned pet dogs and cats is highly prevalent usually with no clinical sign but high density of colonization can be related to gastrointestinal signs. -
Identification of Acanthocephala discovered in changran-pickles and myungran-pickles
Jong-Tai Kim, Jong-Yeol Park, Hun-Su Seo, Hwa-gyun Oh, Jae-Wuk Noh, Sung-Won Kim2 and Hee-Jeong Youn 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 2호 7 111-114 (4 pages)
To identify acanthocephala found in 'Changran-pickles' and 'Myungran-pickles' each organ was measured in permanent slides. In the present report, the results obtained were as follows: 1. Morphology of male worms: Worms possessed 18-19 longitudinal rows, with 4 hooks per row, which became smaller towards the base of proboscis. Each worm contained two testis and six cement glands arranged linearly. Body 22.0 by 0.8-0.6 mm and 15.0 by 0.6-0.4 mm, proboscis 284.8 by 227.6 micro m and 524.9 by 151.4... -
Tissue distribution of bovine viral diarrhea virus antigens in persistently infected cattle
Taekyun Shin, Helen Acland 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 2호 2 81-84 (4 pages)
a tissue pool of liver, kidneys and spleen. Immunohistochemical detection of BVDV showed that BVDV antigens were detected in both epithelial and nonepithelial cells in all examined organs, including the gastrointestinal tract, liver, pancreas, lung, lymphatic organs (spleen, lymph nodes), adrenal gland, ovary, uterus, and the mammary gland. These findings support the hypothesis that animals with persistent BVDV infection spread BVDV through all routes, and that infertility in BVDV infection is... -
Immunohistochemical detection of Prion protein (PrP-Sc) and epidemiological study of BSE in Korea
Hye Cheong Koo, Yong Ho Park, Byeong-Chun Lee, Chanhee Chae, Katherine I. O'Rourke, Timothy V. Baszler 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 1호 4 25-31 (7 pages)
animals with TSEs. In this study, clinical and epidemiological examinations of bovine spongiform encephalopathy (BSE) were conducted in Korea. During the investigated period, none of the cattle exhibited typical clinical signs of BSE, such as behavioral disturbances, high sensitivity, and abnormal locomotion. Immunohistochemical analysis and western immunoblotting were established to detect PrP-Sc in the brain tissue using monoclonal antibody (MAb) F89/160.1.5, produced by immunizing mice with a... -
Morphological structure of accessory spleen in Chinese hamsters
Yeo-Sung Yoon, Jae-Won Shin, Cheol-Beom Park, Yang-Seok Oh, In-Se Lee, Heungshik S. Lee, Joon-Sup Lee 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 2호 2 73-75 (3 pages)
To attempt a rigorous definition of the structure of the accessory spleen (AS) in the Chinese hamster, we examined twenty-one animals, and found AS in 5 animals (23.8%), which were over 7-month-old. The AS had no connection with the main spleen and was seen as a dark red oval organ (0.7 mm x 1.5 mm), which was embedded in the adipose tissue near the tail of the pancreas. It was demarcated from the adipose tissue and some pancreatic tissue. The organ was encapsulated by thin collagenous... -
Capsular polysaccharide typing of domestic mastitis-causing Staphylococcus aureus strains and its potential exploration of bovine mastitis vaccine developmen. I. capsular olysaccharide typing, isolation and purification of the strains
Hong-Ryul Han, Son-Il Pak, Seung-Won Kang, Woo-Seog Jong, Cheol-Jong Youn 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 1호 8 53-60 (8 pages)
autoagglutination in the salt aggregation test. The serotype 336 accounted for 46.7% of the isolates followed by serotype 5 (12.1%) and serotype 8 (12.1%). Particularly, twenty-six (24.3%) isolates reacted with two serotypes; 7 for type 8/336 and 19 for type 5/336. Five isolates (4.7%) were nontypeable with monoclonal antibodies specific for CP serotype 5, 8, or 336. The CP concentration in culture supernatants varied with the serotypes, and the total amount of CP produced by cells grown in a... -
Phenotypic and functional analysis of bovine gd lymphocytes
Yong Ho Park, Han Sang Yoo, Jang Won Yoon, Soo Jin Yang, Jong Sam An, W.C Davis 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 1호 6 39-48 (10 pages)
under these culture conditions that points to potential regulatory and effector functions of gammadelta T cells. The investigations have also provided the information needed to begin identifying and characterizing antigens recognized by the TCR repertoires of WC1+ and WC1- gammadelta T cells. Finally, the investigations have provided the information needed to begin analysis of the mechanisms by which gammadelta T cells modulate MHC restricted immune responses to pathogens and derived vaccines. -
Expression and characterization of the flavoprotein domain of gp91phox
Chang-Hoon Han and Mun-Han Lee 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 1호 3 19-26 (8 pages)
a portion of the highly soluble bacterial protein thioredoxin (TRX). TRX-gp91(phox) (306-569), which contains the putative FAD and NADPH binding sites, showed NADPH-dependent NBT (nitroblue tetrazolium) reductase activity, whereas TRX-gp91(phox) (304-423) and TRX-gp91(phox) (424-569) were inactive. Activity saturated at about a 1:1 molar ratio of FAD to TRX-gp91(phox) (306- 569), and showed the same Km for NADPH as that for superoxide generating activity by the intact enzyme. Activity was not...


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