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Cloning, expression and functional analysis of the duck Toll-like receptor 5 (TLR5) gene
Yuqiang Cheng, Yingjie Sun, Hengan Wang, Shuduan Shi, Yaxian Yan, Jing Li, Chan Ding, Jianhe Sun 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 1호 6 37-46 (10 pages)
Toll-like receptor 5 (TLR5) is responsible for the recognition of bacterial flagellin in vertebrates. In the present study, the first TLR5 gene in duck was cloned. The open reading frame (ORF) of duck TLR5 (dTLR5) cDNA is 2580 bp and encodes a polypeptide of 859 amino acids. We also cloned partial sequences of myeloid differentiation factor 88, 2’-5’-oligoadenylate synthetase (OAS), and myxovirus resistance (Mx) genes from duck. dTLR5 mRNA was highly expressed in the... -
Determining the age of cats by pulp cavity/tooth width ratio using dental radiography
Kyoungsun Park, Jaesang Ahn, Sunmee Kang, Euiri Lee, Soohyun Kim, Sangwan Park, Sungwon Park, Hyunwoo Noh, Kangmoon Seo 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 18 557-561 (5 pages)
The purpose of this study was to evaluate the effect of age on the ratio of pulp cavity/tooth width (P/T ratio) in healthy cats. The dental radiographs of 32 cats (16 males and 16 females) were generated with a digital dental X-ray unit with the animals under general anesthesia. Standardized measurement of the canine teeth was performed by drawing a line on the radiograph perpendicular to the cemento- enamel junction (CEJ) of the tooth. There was an inversely proportional correlation between... -
Efficacy of an oral hyaluronate and collagen supplement as a preventive treatment of elbow dysplasia
Simon Marti-Angulo, Nuria Garcia-Lopez, Ana Diaz-Ramos 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 17 569-574 (6 pages)
One hundred and five Labrador dogs were randomly divided into two groups to determine the number of animals that develop elbow dysplasia when treated with an oral supplement compared to untreated ones. Efficacy of the oral treatment was also evaluated once illness was diagnosed. The supplement (Hyaloral) contained hyaluronic acid, hydrolysed collagen, glucosamine, chondroitin sulphate, and gamma oryzanol. Clinical evaluation of the elbow joints was completed at months 3, 6, 12, and 20 by... -
Use of laparoscopy for diagnosing experimentally induced acute pancreatitis in dogs
Hyun-wook Kim, Ye-in Oh, Ji-hye Choi, Dae-yong Kim, Hwa-young Youn, 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 16 551-556 (6 pages)
Diagnosis of acute pancreatitis in dogs remains a significant challenge despite the development of advanced diagnostic methodologies. Visual inspection and pancreas biopsy using laparoscopy are generally considered to be procedures free of complications when conducted on healthy animals. However, the usefulness of laparoscopy for diagnosing acute pancreatitis has not been assessed. In the present study, the efficacy of laparoscopy for diagnosing acute pancreatitis in dogs was evaluated in... -
Occurrence and characterization of livestock-associated methicillin-resistant Staphylococcus aureus in pig industries of northern Thailand
Prapas Patchanee, , Pakpoom Tadee, Orapun Arjkumpa, David Love, Karoon Chanachai, Thomas Alter, Soawapak Hinjoy, Prasit Tharavich 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 10 529-536 (8 pages)
This study was conducted to determine the prevalence of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) in pigs, farm workers, and the environment in northern Thailand, and to assess LA-MRSA isolate phenotypic characteristics. One hundred and four pig farms were randomly selected from the 21,152 in Chiang Mai and Lamphun provinces in 2012. Nasal and skin swab samples were collected from pigs and farm workers. Environmental swabs (pig stable floor, faucet, and feeder)... -
Use of hydrophilic extra-viral domain of canine distemper virus H protein for enzyme-linked immunosorbent assay development
Ki-hyun Cho, Jeongmi Kim, Hyun-ah Yoo, Dae-hee Kim, Seung-yong Park, Chang-seon Song, In-soo Choi, Joong-bok Lee, 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 6 503-509 (7 pages)
Simple methods for measuring the levels of serum antibody against canine distemper virus (CDV) would assist in the effective vaccination of dogs. To develop an enzyme-linked immunosorbent assay (ELISA) specific for CDV, we expressed hydrophilic extra-viral domain (HEVD) protein of the A75/17-CDV H gene in a pET 28a plasmid-based Escherichia (E.) coli vector system. Expression was confirmed by dot and Western blotting. We proposed that detection of E. coli-expressed H protein might be... -
M gene analysis of canine coronavirus strains detected in Korea
Seok-Young Jeoung, So-Yun Ann, Hyun-Tae Kim, Doo Kim, 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 5 495-502 (8 pages)
The purpose of this study was to investigate the genetic features of canine coronavirus (CCV) strains detected in Korea. M gene sequences obtained for isolates from 22 dogs with enteritis over a 5-year period were evaluated. Sequence comparison revealed that the 22 Korean CCV strains had an 87.2 to 100% nucleotide homology. Comparing to the typical reference CCV strains (type II), the nucleotide sequence of Korean strains had homology ranged from 86.3% to 98.3% (89.1% to 99.2% for the amino acid... -
Protective efficacy of a high-growth reassortant swine H3N2 inactivated vaccine constructed by reverse genetic manipulation
Feng Wen, Ji-Hong Ma, Hai Yu*, Fu-Ru Yang, Meng Huang, Yan-Jun Zhou, Ze-Jun Li, Guang-Zhi Tong* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 6 381-388 (8 pages)
Novel reassortant H3N2 swine influenza viruses (SwIV) with the matrix gene from the 2009 H1N1 pandemic virus have been isolated in many countries as well as during outbreaks in multiple states in the United States, indicating that H3N2 SwIV might be a potential threat to public health. Since southern China is the world’s largest producer of pigs, efficient vaccines should be developed to prevent pigs from acquiring H3N2 subtype SwIV infections, and thus limit the possibility of SwIV infection... -
Prevalence and characteristics of Shiga toxin-producing Escherichia coli (STEC) from cattle in Korea between 2010 and 2011
Eun Kang, Sun Young Hwang, Ka Hee Kwon, Ki Yeon Kim, Jae Hong Kim, Yong Ho Park* 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 5 369-379 (11 pages)
A total of 156 Shiga-like toxin producing Escherichia coli (STEC) were isolated from fecal samples of Korean native (100/568, 18%) and Holstein dairy cattle (56/524, 11%) in Korea between September 2010 and July 2011. Fifty-two STEC isolates (33%) harbored both of shiga toxin1 (stx1) and shiga toxin2 (stx2) genes encoding enterohemolysin (EhxA) and autoagglutinating adhesion (Saa) were detected by PCR in 83 (53%) and 65 (42%) isolates, respectively. By serotyping, six STEC from native cattle and... -
Distribution, quantitative load and characterization of Salmonella associated with swine farms in upper-northern Thailand
Pakpoom Tadee, Kittipong Kumpapong, Danai Sinthuya, Panuwat Yamsakul, Nipa Chokesajjawatee, Supachai Nuanualsuwan, Suchawan Porn 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 20 327-334 (8 pages)
This study was conducted to analyze the prevalence and quantitative loads of Salmonella spp. on pig farms in Chiang Mai, Lamphun, Thailand to assess loading levels before slaughtering. The serotype diversity, antimicrobial-resistance pattern and pulse-field type of Salmonella spp. were also characterized to assess the dynamic propagation of the pathogen. The Salmonella-positive prevalence was 246/805 (30.56%), and the quantitative loads varied from 1.48∼4.04 Log10MPN/g, with a mean ± standard...


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