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Cytotoxicity of gamma-ray in rat immature hippocampal neurons
Miyoung Yang, Myoung-Sub Song, Sung-Ho Kim, Jong-Choon Kim, Joong-Sun Kim, Taekyun Shin, Changjong Moon* 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 1 203-207 (5 pages)
This in vitro study evaluated the detrimental effect of acute gamma (g)-irradiation on rat immature hippocampal neurons. Rat immature hippocampal neurons (0.5 day in vitro) were irradiated with 0∼4 Gy g-rays. Cytotoxicity was analyzed using a lactate dehydrogenase release assay at 24 h after g-irradiation. Radiation-induced cytotoxicity in immature hippocampal neurons increased in a dose- dependent manner. Pre-treatments of pro-apoptotic caspase inhibitors and anti-oxidative substances... -
Real time observation of mouse fetal skeleton using a high resolution X-ray synchrotron
Dong Woo Chang, Bora Kim, Jae Hoon Shin, Young Min Yun, Jung Ho Je, Yeu kuang Hwu, Jung Hee Yoon, Je Kyung Seong 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 1 107-114 (8 pages)
The X-ray synchrotron is quite different from conventional radiation sources. This technique may expand the capabilities of conventional radiology and be applied in novel manners for special cases. To evaluate the usefulness of X-ray synchrotron radiation systems for real time observations, mouse fetal skeleton development was monitored with a high resolution X-ray synchrotron. A non-monochromatized X-ray synchrotron (white beam, 5C1 beamline) was employed to observe the skeleton of mice under... -
Inhibitory effects of interleukin-10 plasmid DNA on the development of atopic dermatitis-like skin lesions in NC/Nga mice
Bock-Gie Jung, Sun-Ju Cho, Jae-Hyung Ko, Bong-Joo Lee* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 6 213-220 (8 pages)
Interleukin (IL)-10 exerts potent anti-inflammatory effects by suppression of both T-help (Th) 1 and Th2 cells. Previous studies have reported that IL-10 can ameliorate various inflammatory disorders. The present study was performed to examine whether IL-10 plasmid DNA could suppress development of atopic dermatitis (AD)-like skin lesions in NC/Nga mice, as an initial step towards the development of an appliance for use in dogs with AD. Intradermal injection of IL-10 plasmid DNA markedly... -
Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury
Hak-Hyun Ryu, Ji-Hey Lim, Ye-Eun Byeon, Jeong-Ran Park, Min-Soo Seo, Young-Won Lee, Wan Hee Kim, Kyung-Sun Kang*, Oh-Kyeong Kweo 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 4호 1 273-284 (12 pages)
In this study, we evaluated if the implantation of allogenic adipose-derived stem cells (ASCs) improved neurological function in a canine spinal cord injury model. Eleven adult dogs were assigned to three groups according to treatment after spinal cord injury by epidural balloon compression: C group (no ASCs treatment as control), V group (vehicle treatment with PBS), and ASC group (ASCs treatment). ASCs or vehicle were injected directly into the injured site 1 week after spinal cord injury.... -
Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress
Mi Na Lee, Sang Hun Lee, Min Young Lee, Yun Hee Kim, Jae Hong Park, Jung Min Ryu, Seung Pil Yun, Yu Jin Lee, Mi Ok Kim, Kwangsun 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 3호 4 247-256 (10 pages)
Oxidative stresses induced by reactive oxygen species (ROS) have been shown to be involved in several physiological and pathophysiological processes, such as cell proliferation and differentiation. Steroid hormones can protect cells against apoptosis or induce cell proliferation by several mechanisms. Among androgenic hormones, dihydrotestosterone (DHT) is generated by a 5a- reduction of testosterone. Unlike testosterone, DHT cannot be aromatized to estradiol, therefore DHT is considered a pure... -
Immunohistochemical expression of h-telomerase reverse transcriptase in canine and feline meningiomas
Luciana Mandrioli, Serena Panarese, Alessandro Cesari, Maria Teresa Mandara, Paolo Stefano Marcato, Giuliano Bettini 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 2 111-115 (5 pages)
Telomere length maintenance is regarded as a fundamental step in tumorigenesis, as most human brain tumors, including meningiomas, stabilize the ends of their chromosomes using telomerase. This investigation represents an introduction to telomerase expression in canine and feline meningiomas. Twenty-five archived cases (14 dogs and 11 cats) were immunohistochemically tested for human-telomerase reverse transcriptase (h- TERT), scored, and quantified; furthermore, mitoses were counted on sections... -
Development of a monoclonal antibody-based co-agglutination test to detect enterotoxigenic Escherichia coli isolated from diarrheic neonatal calves
Brajesh C. Varshney, N.M. Ponnanna, Pranati A. Sarkar, Pragna Rehman, Jigar H. Shah 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 9 57-64 (8 pages)
Escherichia coli (E. coli) strains were collected from young diarrheic calves in farms and field. Strains that expressed the K99 (F5) antigen were identified by agglutination tests using reference antibodies to K99 antigen and electron microscopy. The K99 antigen from a selected field strain (SAR-14) was heat-extracted and fractionated on a Sepharose CL-4B column. Further purification was carried out by sodium deoxycholate treatment and/or ion-exchange chromatography. Monoclonal antibodies to... -
Increased phosphorylation of c-Jun NH (2)-terminal protein kinase in the sciatic nerves of Lewis rats with experimental autoimmune neuritis
Meejung Ahn, Taekyun Shin 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 2 13-17 (5 pages)
The phosphorylation of c-Jun NH (2)-terminal protein kinase (JNK), one of the mitogen-activated protein kinases, was analyzed in the sciatic nerves of Lewis rats with experimental autoimmune neuritis (EAN).Western blot analysis showed that the expression levels of both phosphorylated JNK1 (p-JNK1, approximately 46 kDa)and phosphorylated JNK2 (p-JNK2, approximately 54 kDa)in the sciatic nerves of rats with EAN increased significantly (p <0.05)at day 14 post-immunization (PI)and remained at this... -
Use of the n-butyl cyanoacrylate adhesive and the polyglactine thread suture for corneal rhaphy in rabbit (Oryctolagus cunicullus)
Francisco Claudio Dantas Mota, Duvaldo Eurides, Patricia Maria Coletto Freitas, Marcelo Emilio Beletti, Michelle RodriquesGoular 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 3호 14 267-270 (4 pages)
The aim of this study was to evaluate the cicatricial repair of perforating cornea in rabbits, by using the N-butyl cyanoacrylate adhesive compared to the 910- polyglactine thread suture through macroscopic and histological assays. Corneas from 18 adult rabbits were perforated and subsequently occluded with N-butyl cyanoacrylate synthetic adhesive (right cornea) or by separated single points using the 910-polyglactine thread (left cornea). The rabbits were divided into groups containing three... -
Effect of β -mercaptoethanol or epidermal growth factor supplementation on in vitro maturation of canine oocytes collected from dogs with different stages of the estrus cycle
Min Kyu Kim, Yuda Heru Fibrianto, Hyun Ju Oh, Goo Jang, Hye Jin Kim, Kyu Seung Lee, Sung Keun Kang, Byeong Chun Lee, Woo Suk Hwa 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 3호 11 253-258 (6 pages)
Supplementation of β-mercaptoethanol (β-ME) in in vitro maturation (IVM) medium was shown to improve embryo development and quality in several species. Epidermal growth factor (EGF) was also shown to improve IVM of human oocyte and embryo development after in vitro fertilization (IVF). The effect of these two compounds were suggested to be mediated through the synthesis of glutathione (GSH) which is known to play an important role in protecting the cell or embryos from oxidative damage. Thus,...
