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211.

Development of a multiplex loop-mediated isothermal amplification assay to detect shiga toxin-producing Escherichia coli in cattle

Hee-Jin Dong, Ae-Ri Cho, Tae-Wook Hahn, Seongbeom Cho* 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 19 317-325 (9 pages)

Hee-Jin Dong, Ae-Ri Cho, Tae-Wook Hahn, Seongbeom Cho* 저
...
- 3,000원
A multiplex loop-mediated isothermal amplification (mLAMP) assay was developed for simultaneous detection of the stx1 and stx2 genes and applied for detection of shiga toxin-producing Escherichia coli (STEC) in cattle farm samples. Two target genes were distinguished based on Tm values of 85.03 ± 0.54C for stx1 and 87.47 ± 0.35C for stx2. The mLAMP assay was specific (100% inclusivity and exclusivity), sensitive (with a detection limit as low as 10 fg/μL), and quantifiable (R = 0.9313). The...
212.

Equine hyperimmune serum protects mice against Clostridium difficile spore challenge

Weiwei Yan, Kang-Soon Shin, Shih-Jon Wang, Hua Xiang, Thomas Divers, Sean McDonough, James Bowman, Anne Rowlands, Bruce Akey, Hu 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 11 249-258 (10 pages)

Weiwei Yan, Kang-Soon Shin, Shih-Jon Wang, Hua Xiang, Thomas Divers, Sean McDonough, James Bowman, Anne Rowlands, Bruce Akey, Hu 저
...
- 3,000원
Clostridium (C.) difficile is a common cause of nosocomial diarrhea in horses. Vancomycin and metronidazole have been used as standard treatments but are only moderately effective, which highlights the need for a novel alternative therapy. In the current study, we prepared antiserum of equine origin against both C. difficile toxins A and B as well as whole-cell bacteria. The toxin-neutralizing activities of the antibodies were evaluated in vitro and the prophylactic effects of in vivo passive...
213.

A novel low-cost method for Mycobacterium avium subsp. paratuberculosis DNA extraction from an automated broth culture system for real-time PCR analysis

Miguel Salgado*, Cristobal Verdugo, Cord Heuer, Pedro Castillo, Patricia Zamorano 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 9 233-239 (7 pages)

Miguel Salgado*, Cristobal Verdugo, Cord Heuer, Pedro Castillo, Patricia Zamorano 저
...
- 3,000원
PCR is a highly accurate technique for confirming the presence of Mycobacterium avium subsp. paratuberculosis (Map) in broth culture. In this study, a simple, efficient, and low-cost method of harvesting DNA from Map cultured in liquid medium was developed. The proposed protocol (Universidad Austral de Chile [UACH]) was evaluated by comparing its performance to that of two traditional techniques (a QIAamp DNA Stool Mini Kit and cethyltrimethylammonium bromide [CTAB] method). The results were...
214.

Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus

Lishan Lv, Xiaoming Li, Genmei Liu, Ran Li, Qiliang Liu, Huifang Shen, Wei Wang, Chunyi Xue, Yongchang Cao* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 6 209-216 (8 pages)

Lishan Lv, Xiaoming Li, Genmei Liu, Ran Li, Qiliang Liu, Huifang Shen, Wei Wang, Chunyi Xue, Yongchang Cao* 저
...
- 3,000원
Infectious bronchitis virus (IBV) poses a severe threat to the poultry industry and causes heavy economic losses worldwide. Vaccination is the most effective method of preventing infection and controlling the spread of IBV, but currently available inactivated and attenuated virus vaccines have some disadvantages. We developed a chimeric virus-like particle (VLP)-based candidate vaccine for IBV protection. The chimeric VLP was composed of matrix 1 protein from avian influenza H5N1 virus and a...
215.

Recombinant Kluyveromyces lactis expressing highly pathogenic porcine reproductive and respiratory syndrome virus GP5 elicits mucosal and cell-mediated immune responses in mice

Haiyan Zhao, Yalan Wang, Zhitao Ma, Yongqiang Wang, Wen-hai Feng* 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 5 199-208 (10 pages)

Haiyan Zhao, Yalan Wang, Zhitao Ma, Yongqiang Wang, Wen-hai Feng* 저
...
- 3,000원
Currently, killed-virus and modified-live porcine reproductive and respiratory syndrome virus (PRRSV) vaccines are used to control porcine reproductive and respiratory syndrome. However, both types of vaccines have inherent drawbacks; accordingly, the development of novel PRRSV vaccines is urgently needed. Previous studies have suggested that yeast possesses adjuvant activities, and it has been used as an expression vehicle to elicit immune responses to foreign antigens. In this report,...
216.

Positive effects of porcine IL-2 and IL-4 on virus-specific immune responses induced by the porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 DNA vaccine in swine

Deyuan Tang*, Jian Liu, Chunyan Li, Hua Zhang, Ping Ma, Xianfeng Luo, Zhiyong Zeng, Nining Hong, Xia Liu, Bin Wang, Feng Wang, Z 대한수의학회 Journal of Veterinary Science 11 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 11 99-109 (11 pages)

Deyuan Tang*, Jian Liu, Chunyan Li, Hua Zhang, Ping Ma, Xianfeng Luo, Zhiyong Zeng, Nining Hong, Xia Liu, Bin Wang, Feng Wang, Z 저
...
- 4,000원
The purpose of this study was to investigate the effects of porcine interleukin (IL)-2 and IL-4 genes on enhancing the immunogenicity of a porcine reproductive and respiratory syndrome virus ORF5 DNA vaccine in piglets. Eukaryotic expression plasmids pcDNA-ORF5, pcDNA-IL-2, and pcDNA-IL-4 were constructed and then expressed in Marc-145 cells. The effects of these genes were detected using an indirect immunofluorescent assay and reverse transcription polymerase chain reaction (RT-PCR)....
217.

Elucidating the role of ApxI in hemolysis and cellular damage by using a novel apxIA mutant of Actinobacillus pleuropneumoniae serotype 10

Nai-Yun Chang, Zeng-Weng Chen, Ter-Hsin Chen, Jiunn-Wang Liao, Cheng-Chung Lin, Maw-Sheng Chien, Wei-Cheng Lee, Jiunn-Horng Lin, 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 9 81-89 (9 pages)

Nai-Yun Chang, Zeng-Weng Chen, Ter-Hsin Chen, Jiunn-Wang Liao, Cheng-Chung Lin, Maw-Sheng Chien, Wei-Cheng Lee, Jiunn-Horng Lin, 저
...
- 3,000원
(A.) pleuropneumoniae (Apx) play major roles in the pathogenesis of pleuropneumonia in swine. This study investigated the role of ApxI in hemolysis and cellular damage using a novel apxIA mutant, ApxIA336, which was developed from the parental strain A. pleuropneumoniae serotype 10 that produces only ApxI in vitro. The genotype of ApxIA336 was confirmed by PCR, Southern blotting, and gene sequencing. Exotoxin preparation derived from ApxIA336 was analyzed for its bioactivity towards porcine...
218.

An overview of calf diarrhea - infectious etiology, diagnosis, and intervention

Yong-il Cho, Kyoung-Jin Yoon* 대한수의학회 Journal of Veterinary Science 17 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 1 1-17 (17 pages)

Yong-il Cho, Kyoung-Jin Yoon* 저
...
- 4,600원
Calf diarrhea is a commonly reported disease in young animals, and still a major cause of productivity and economic loss to cattle producers worldwide. In the report of the 2007 National Animal Health Monitoring System for U.S. dairy, half of the deaths among unweaned calves was attributed to diarrhea. Multiple pathogens are known or postulated to cause or contribute to calf diarrhea development. Other factors including both the environment and management practices influence disease severity or...
219.

Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis

Alfredo García, Remigio Martínez, José Manuel Benitez-Medina, David Risco, Waldo Luis García, Joaqu& 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 17 491-494 (4 pages)

Alfredo García, Remigio Martínez, José Manuel Benitez-Medina, David Risco, Waldo Luis García, Joaqu& 저
...
- 1,500원
Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus(D.)congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tuberculosis, Staphylococcus aureus, or Austwickia chelonae was observed. Finally, the RT-PCR assay was used...
220.

Prevalence and risk factors for carriage of multi-drug resistant Staphylococci in healthy cats and dogs

Paola Gandolfi-Decristophoris, Gertraud Regula, Orlando Petrini, Jakob Zinsstag, Esther Schelling 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 11 449-456 (8 pages)

Paola Gandolfi-Decristophoris, Gertraud Regula, Orlando Petrini, Jakob Zinsstag, Esther Schelling 저
...
- 3,000원
species and determined the prevalence of multi-drug resistance in healthy cats and dogs. Risk factors associated with the carriage of multi-drug resistant strains were explored. Isolates from 256 dogs and 277 cats were identified at the species level using matrix-assisted laser desorption ionisation-time of flight mass spectrometry. The diversity of coagulase-negative Staphylococci (CNS) was high, with 22 species in dogs and 24 in cats. Multi-drug resistance was frequent (17%) and not always...

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