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Genetic Reassortment of Rice stripe virus RNA Segments Detected by RT-PCR Restriction Enzyme Analysis-based Method
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 2호 7 148-155 (8 pages)
Rice stripe virus (RSV) suggested possible genetic reassortment of RNA segments, but whether this RNA variation contributed to the recent RSV outbreaks in Korea is yet unclear. To further clarify these RSVRNA segment variations, we developed a reverse transcription- polymerase reaction/restriction enzyme (RTPCR/ RE) analysis-based method. We identified five REs, including DraI, EcoR1, NdeI/AseI, and SpeI, that could differentiate RSV RNA 1-4 subtypes, respectively. Our RT-PCR/RE results provided... -
Screening of Rhizobacteria for Biological Control of Cucumber Root and Crown Rot Caused by Phytophthora drechsleri
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 1호 12 78-84 (7 pages)
more specifically fluorescent pseudomonads and certain species of Bacillus, are known as biocontrol agents of fungal root diseases of agronomic crops. In this study, 144 bacteria were isolated from cucumber rhizosphere and screened as potential biological control agents against Phytophthora drechsleri, the causal agent of cucumber root rot, in vitro condition. Non-volatile compounds of 23 isolates showed noticeable inhibition zone (> 30%) against P. drechsleri, whereas volatile compounds of 7... -
Generation of Antibodies Against Rice stripe virus Proteins Based on Recombinant Proteins and Synthetic Polypeptides
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 1호 7 37-43 (7 pages)
fields for RSV detection. In this study, RSV proteins that were derived from recombinant proteins and synthetic polypeptides as antigens were generated and were raised in rabbits for antiserum production. Among seven proteins in RSV, genes that code for NCP and NS3 proteins were cloned and subcloned into vector carrying His-tag protein and were expressed in E. coli. Of two recombinant proteins, only anti-NCP displayed stable hybridization signals in western blot analysis. Alternately, synthetic... -
Effect of Alternaria solani Exudates on Resistant and Susceptible Potato Cultivars from Two Different pathogen isolates
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 1호 3 14-19 (6 pages)
ubiquitous disease in many countries around the world. Our previous screening of several Iranian potato cultivars found that significant variation in resistance phenotypes exists between two cultivars: resistant ``Diamond`` and susceptible ``Granula``. Our previous analysis of five different pathogen isolates also identified varying degrees of aggressiveness regardless of the host cultivar. Here, a bioassay was used to study the role of liquid culture exudates produced in vitro on pathogenicity... -
Efficacy of Fluopicolide against Phytophthora capsici Causing Pepper Phytophthora Blight
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 4호 10 367-371 (5 pages)
In this study, we evaluated the efficacy of fluopicolide to inhibit Phytophthora capsici in vitro, and to control pepper Phytophthora blight in a greenhouse and pepper fields. Fluopicolide was tested on various developmental stages of P. capsici 06-143 (a sensitive isolate to metalaxyl) and JHAW1-2 (a resistant isolate to metalaxyl). Mycelial growth and zoosporangium germination of both isolates were completely inhibited at 4.0 μg/ml of the fungicide in vitro. The EC50 (effective concentrations... -
Antifungal Activity of Lower Alkyl Fatty Acid Esters against Powdery Mildews
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 4호 9 360-366 (7 pages)
In the course of a searching environmental friendly antifungal compounds, we found that mixture of methyl esters of fatty acids obtained from soybean oil had potent control efficacy against barley powdery mildew (Blumeria graminis f. sp. hordei). In this study, ten alkyl fatty acid esters (AFAEs) were tested for in vivo antifungal activity against five plant diseases such as rice blast, rice sheath blight, tomato gray mold, tomato late blight and barley powdery mildew. Some AFAEs showed the most... -
Genetic Stability of Magnaporthe oryzae during Successive Passages through Rice Plants and on Artificial Medium
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 4호 3 313-320 (8 pages)
a major factor underlying the rapid breakdown of host resistance in the field. However, little information is available on the mechanism of genetic instability. In this study, we assessed the stability of repetitive DNA elements and several key phenotypic traits important for pathogenesis after serially transferring two isolates though rice plants and an artificial medium. Using isolate 70-15, we obtained a total of 176 single-spore isolates from 10 successive rounds of culturing on artificial... -
Control Efficacy of Phloretin Isolated from Apple Fruits Against Several Plant Diseases
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 3호 13 280-285 (6 pages)
by Colletotrichum coccodes also was moderately suppressed. However, rice sheath blight (Rhizoctonia solani AG1), and barley powdery mildew (Blumeria graminis f. sp. hordei) were hardly controlled. In addition, the compound showed in vitro antifungal activity against some plant pathogenic fungi including Phytophthora capsici, Alternaria panax, Sclerotinia sclerotiorum, R. solani AG4, and M. grisea. This is the first report on the antifungal activity of phloretin against plant pathogenic fungi. -
Potential of the Volatile-Producing Fungus Nodulisporium sp. CF016 for the Control of Postharvest Diseases of Apple
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 3호 7 253-259 (7 pages)
In vitro and in vivo mycofumigation effects of the volatileproducing fungus Nodulisporium sp. CF016 isolated from stem of Cinnamomum loureirii and the role of its volatile compounds were investigated against phytopathogenic fungi. The volatile compounds produced by Nodulisporium sp. CF016 inhibited and killed a wide range of plant and storage pathogens including to Pythium ultimum, Rhizoctonia solani, Fusarium oxysporum, Phytophthora capsici, Sclerotinia sclerotiorum, Colletotrichum coccodes,... -
Production of a Phytotoxic Compound, 3-Phenylpropionic Acid by a Bacterial Endophyte, Arthrobacter humicola YC6002 Isolated from the Root of Zoysia japonica
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 3호 6 245-252 (8 pages)
An endophytic bacterial strain, Arthrobacter humicola YC6002, was isolated from a surface sterilized root of Korean turf grass (Zoysia japonica) collected from Jinju, Korea. This strain showed inhibitory effect on germination and shoot growth of radish. The inhibition of germination and shoot growth of radish seeds varied depending on the age of culture and the temperature at which it was incubated. The culture filtrate of 1/10- strength Tryptic Soy Broth medium, incubated for 48 hours at 30


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