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221.

Novel Sources of Resistance to Phytophthora capsici on Pepper (Capsicum sp.) Landraces from Mexico

Jes?s Enrique Retes-Manjarrez, Walter Arturo Rubio-Arag?n, Isidro M?rques-Zequera, Isabel Cruz-Lachica, Raymundo Sa?l Garc?a-Estrada, Ousmane Sy 한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2020, 36권 6호 7 600-607 (8 pages)

Jes?s Enrique Retes-Manjarrez, Walter Arturo Rubio-Arag?n, Isidro M?rques-Zequera, Isabel Cruz-Lachica, Raymundo Sa?l Garc?a-Estrada, Ousmane Sy 저
...
- 3,000원
Phytophthora capsici Leonian is a major pathogen of pepper worldwide and few resistance sources to this pathogen have been identified so far. The goals of this study were to identify new sources of resistance against P. capsici in Capsicum landraces and analyze the relationship between the resistance indicator of plant symptoms and some plant phenotype parameters of plant height, stem width, leaf length and leaf width. Thirtytwo landraces of pepper were collected from fourteen states in Mexico....
222.

Rapid and Efficient Detection of 16SrI Group Areca Palm Yellow Leaf Phytoplasma in China by Loop-Mediated Isothermal Amplification

Shao-shuai Yu, Hai-yan Che, Sheng-jie Wang, Cai-li Lin, Ming-xing Lin, Wei-wei Song, Qing-hua Tang, Wei Yan, Wei-quan Qin 한국식물병리학회 The Plant Pathology Journal 9 Pages

한국식물병리학회 The Plant Pathology Journal 2020, 36권 5호 8 459-467 (9 pages)

Shao-shuai Yu, Hai-yan Che, Sheng-jie Wang, Cai-li Lin, Ming-xing Lin, Wei-wei Song, Qing-hua Tang, Wei Yan, Wei-quan Qin 저
...
- 3,000원
and Wanning counties in Hainan province using the two sets of LAMP primers 16SrDNA-2 and 16SrDNA-3, whereas no phytoplasma was detected in the negative control. The LAMP method established in this study with comparatively high sensitivity and stability, provides reliable results that could be visually detected, making it suitable for application and research in rapid diagnosis of AYL disease, detection of seedlings with the pathogen and breeding of diseaseresistant Areca palm varieties.
223.

Development of a Daily Epidemiological Model of Rice Blast Tailored for Seasonal Disease Early Warning in South Korea

Kwang-Hyung Kim, Imgook Jung 한국식물병리학회 The Plant Pathology Journal 12 Pages

한국식물병리학회 The Plant Pathology Journal 2020, 36권 5호 3 406-417 (12 pages)

Kwang-Hyung Kim, Imgook Jung 저
...
- 4,100원
into a realistic and measurable disease value through statistical analyses with 13 rice blast incidence datasets, and subsequently validated using the data from another rice blast experiment conducted in Icheon, South Korea, from 1974 to 2000. The sensitivity of the model to air temperature, relative humidity, and precipitation input variables was examined, and the relative humidity resulted in the most sensitive response from the model. Overall, our results indicate that the EPIRICE Daily Risk...
224.

Rapid Detection of Lily mottle virus and Arabis mosaic virus Infecting Lily (Lilium spp.) Using Reverse Transcription Loop-Mediated Isothermal Amplification

Yubao Zhang, Yajun Wang, Zhongkui Xie, Ruoyu Wang, Zhihong Guo, Yuhui He 한국식물병리학회 The Plant Pathology Journal 9 Pages

한국식물병리학회 The Plant Pathology Journal 2020, 36권 2호 6 170-178 (9 pages)

Yubao Zhang, Yajun Wang, Zhongkui Xie, Ruoyu Wang, Zhihong Guo, Yuhui He 저
...
- 3,000원
cross-reactivity from other lily viruses. Optimal conditions for LAMP reactions were 65°C and 60°C for 60 min for LMoV and ArMV, respectively. Detection sensitivity for both RT-LAMP assays was a hundredfold greater than that of our comparative RT-polymerase chain reaction as- says. We have also found this relatively rapid, target specific and sensitive method can also be used for samples collected in the field and may be especially useful in regions with limited or no laboratory facilities.
225.

Rapid and Sensitive Detection of Lettuce Necrotic Yellows Virus and Cucumber Mosaic Virus Infecting Lettuce (Lactuca sativa L.) by Reverse Transcription Loop-Mediated Isothermal Amplification

Yubao Zhang, Zhongkui Xie, John D Fletcher, Yajun Wang, Ruoyu Wang, Zhihong Guo, Yuhui He 한국식물병리학회 The Plant Pathology Journal 11 Pages

한국식물병리학회 The Plant Pathology Journal 2020, 36권 1호 7 76-86 (11 pages)

Yubao Zhang, Zhongkui Xie, John D Fletcher, Yajun Wang, Ruoyu Wang, Zhihong Guo, Yuhui He 저
...
- 4,000원
Optimum LAMP reactions were conducted in betaine-free media with 6 mM Mg2+ at 65°C for LNYV and 60°C for 60 min for CMV, respectively. The detection limit was 3.5 pg/ml and 20 fg/ml using RT-LAMP for LNYV and CMV plasmids, respectively. Detection sensitivity for both RT-LAMP assays was greater by a factor of 100 compared to the conventional reverse transcription polymerase chain reaction assays. This rapid, specific, and sensitive technique should be more widely applied due to its low cost and...
226.

Identification of Heterodera glycines (Tylenchida; Heteroderidae) Using qPCR

Hyoung-Rai Ko, Heonil Kang, Eun-Hyoung Park, Eun-Hwa Kim, Jae-Kook Lee 한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 6호 11 654-661 (8 pages)

Hyoung-Rai Ko, Heonil Kang, Eun-Hyoung Park, Eun-Hwa Kim, Jae-Kook Lee 저
...
- 3,000원
from a single cyst and single second-stage juvenile (J2). The specificity was confirmed by the absence of SYBR fluorescent signals of three other Heterodera species. A serial dilution of DNA extracted from a single cyst was obtained for the sensitivity test. The result showed that the standard curve of the test had a highly significant linearity between DNA concentration and Ct value (R2 = 0.996, slope = −3.49) and that the detection limit concentration of DNA of the primer set was 10 pg...
227.

Loop-Mediated Isothermal Amplification for the Detection of Xanthomonas arboricola pv. pruni in Peaches

Weilan Li, Seung-Yeol Lee, Chang-Gi Back, Leonid N. Ten, Hee-Young Jung 한국식물병리학회 The Plant Pathology Journal 9 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 6호 9 635-643 (9 pages)

Weilan Li, Seung-Yeol Lee, Chang-Gi Back, Leonid N. Ten, Hee-Young Jung 저
...
- 3,000원
loopmediated isothermal amplification (LAMP) detection method were developed. The LAMP assay was designed to test crude plant tissue without pre-extraction, or heating incubation, and without advanced analysis equipment. The LAMP primers were designed by targeting an ABC transporter ATP-binding protein, this primer set was tested using the genomic DNA of Xanthomonas and non-Xanthomonas strains, and a ladder product was generated from the genomic DNA of X. arboricola pv. pruni strain but not from...
228.

Hydrogen Peroxide Prompted Lignification Affects Pathogenicity of Hemi-biotrophic Pathogen Bipolaris sorokiniana to Wheat

Ajit Poudel, Sudhir Navathz, Ramesh Chand, Vinod K. Mishra, Pawan K. Singh, Arun K. Joshi 한국식물병리학회 The Plant Pathology Journal 14 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 4호 1 287-300 (14 pages)

Ajit Poudel, Sudhir Navathz, Ramesh Chand, Vinod K. Mishra, Pawan K. Singh, Arun K. Joshi 저
...
- 4,300원
to spot blotch in wheat. We used cluster analysis to separate the resistant and susceptible genotypes by phenotypic and biochemical traits. H2O2 associated lignin production significantly reduced the number of appressoria and penetration pegs. We visualized the effect of lignin in disease resistance using differential histochemical staining of tissue from resistant and susceptible genotypes, which shows the variable accumulation of hydrogen peroxide and lignin around penetration sites.
229.

A Duplex PCR Assay for Rapid Detection of Phytophthora nicotianae and Thielaviopsis basicola

Na Liu, Shijun Jiang, Songli Feng, Wenyan Shang, Guozhen Xing, Rui Qiu, Chengjun Li, Shujun Li, Wenming Zheng 한국식물병리학회 The Plant Pathology Journal 6 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 2호 9 172-177 (6 pages)

Na Liu, Shijun Jiang, Songli Feng, Wenyan Shang, Guozhen Xing, Rui Qiu, Chengjun Li, Shujun Li, Wenming Zheng 저
...
- 3,000원
using isolates of P. nicotianae, T. basicola and control samples. The results showed that the target pathogens could be detected from diseased tobacco plants by a combination of the specific primers. The sensitivity limitation was 100 fg/μl of pure genomic DNA of the pathogens. This new assay can be applied to screen out target pathogens rapidly and reliably in one PCR and will be an important tool for the identification and precise early prediction of these two destructive diseases of tobacco.
230.

Visualization of Phytophthora palmivora Infection in Oil Palm Leaflets with Fluorescent Proteins and Cell Viability Markers

Juan C. Ochoa, Mariana Herrera, M?nica Navia, Hern?n Mauricio Romero 한국식물병리학회 The Plant Pathology Journal 13 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 1호 3 19-31 (13 pages)

Juan C. Ochoa, Mariana Herrera, M?nica Navia, Hern?n Mauricio Romero 저
...
- 4,200원
using Agrobacterium tumefaciens-mediated transformation (ATMT) to include the fluorescent proteins CFPSKL (peroxisomal localization), eGFP and mRFP1 (cytoplasmic localization). The stability of some transformants was confirmed by Southern blot analysis and single zoospore cultures; additionally, virulence and in vitro growth were compared to the wild-type isolate to select transformants with the greatest resemblance to the WT isolate. GFP-tagged P. palmivora was useful to identify all of the...

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