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221.

Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis

Alfredo García, Remigio Martínez, José Manuel Benitez-Medina, David Risco, Waldo Luis García, Joaqu& 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 17 491-494 (4 pages)

Alfredo García, Remigio Martínez, José Manuel Benitez-Medina, David Risco, Waldo Luis García, Joaqu& 저
...
- 1,500원
Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus(D.)congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tuberculosis, Staphylococcus aureus, or Austwickia chelonae was observed. Finally, the RT-PCR assay was used...
222.

Development of a novel enzyme-linked immunosorbent assay to detect anti-IgG against swine hepatitis E virus

Won Jung Lee, Min Kyoung Shin, Seung Bin Cha, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 13 467-472 (6 pages)

Won Jung Lee, Min Kyoung Shin, Seung Bin Cha, Han Sang Yoo 저
...
- 3,000원
Swine hepatitis E virus (HEV) is widespread throughout pigs in both developing and industrialized countries. This virus is an important zoonotic agent and a public concern worldwide. Infected pigs are asymptomatic, so diagnosing swine HEV relies on detection of the virus or antibodies against the virus. However, several obstacles need to be overcome for effective and practical serological diagnosis. In this study, we developed an enzyme-linked immunosorbent assay (ELISA) that used a purified...
223.

Genetic diversity of Korean Bacillus anthracis isolates from soil evaluated with a single nucleotide repeat analysis

Sang Hoon Kim, Kyoung Hwa Jung, Se Kye Kim, Seong-Joo Kim, Ji-Cheon Kim, Soo Young Cho, Jin Choul Chai, Young Seek Lee, Yun Ki K 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 12 457-465 (9 pages)

Sang Hoon Kim, Kyoung Hwa Jung, Se Kye Kim, Seong-Joo Kim, Ji-Cheon Kim, Soo Young Cho, Jin Choul Chai, Young Seek Lee, Yun Ki K 저
...
- 3,000원
Bacillus (B.) anthracis, the etiological agent of anthrax, is one of the most genetically monomorphic bacteria species in the world. Due to the very limited genetic diversity of this species, classification of isolates of this bacterium requires methods with high discriminatory power. Single nucleotide repeat (SNR) analysis is a type of variable-number tandem repeat assay that evaluates regions with very high mutation rates. To subtype a collection of 21 isolates that were obtained during a...
224.

Prevalence and risk factors for carriage of multi-drug resistant Staphylococci in healthy cats and dogs

Paola Gandolfi-Decristophoris, Gertraud Regula, Orlando Petrini, Jakob Zinsstag, Esther Schelling 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 11 449-456 (8 pages)

Paola Gandolfi-Decristophoris, Gertraud Regula, Orlando Petrini, Jakob Zinsstag, Esther Schelling 저
...
- 3,000원
We investigated the distribution of commensal staphylococcal species and determined the prevalence of multi-drug resistance in healthy cats and dogs. Risk factors associated with the carriage of multi-drug resistant strains were explored. Isolates from 256 dogs and 277 cats were identified at the species level using matrix-assisted laser desorption ionisation-time of flight mass spectrometry. The diversity of coagulase-negative Staphylococci (CNS) was high, with 22 species in dogs and 24 in...
225.

Codon optimization of the rabbit hemorrhagic disease virus (RHDV) capsid gene leads to increased gene expression in Spodoptera frugiperda 9 (Sf9) cells

Jingpeng Gao, Chunchun Meng, Zongyan Chen, Chuanfeng Li, Guangqing Liu 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 10 441-447 (7 pages)

Jingpeng Gao, Chunchun Meng, Zongyan Chen, Chuanfeng Li, Guangqing Liu 저
...
- 3,000원
Rabbit hemorrhagic disease (RHD) is contagious and highly lethal. Commercial vaccines against RHD are produced from the livers of experimentally infected rabbits. Although several groups have reported that recombinant subunit vaccines against rabbit hemorrhagic disease virus (RHDV) are promising, application of the vaccines has been restricted due to high production costs or low yield. In the present study, we performed codon optimization of the capsid gene to increase the number of preference...
226.

Emergence of virulent pseudorabies virus infection in Northern China

Rui Wu, Chaoyong Bai, Jinzhong Sun, Shengke Chang, Xuke Zhang 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 17 363-365 (3 pages)

Rui Wu, Chaoyong Bai, Jinzhong Sun, Shengke Chang, Xuke Zhang 저
...
- 1,500원
Our investigation was conducted in order to verify a recent severe epidemic at several swine farms in northern China that indicated a newly emerging disease. Evidence confirmed that the epidemic was caused by a virulent Pseudorabies virus infection in swine herds.
227.

Evaluation of changes in left ventricular myocardial function observed in canine myocardial dysfunction model using a two-dimensional tissue tracking technique

Lina Hamabe, Ryuji Fukushima, Keisuke Kawamura, Yusuke Shinoda, Hsu Huai-Che, Shuji Suzuki, Derya Aytemiz, Toshiroh Iwasaki, Ryo 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 16 355-362 (8 pages)

Lina Hamabe, Ryuji Fukushima, Keisuke Kawamura, Yusuke Shinoda, Hsu Huai-Che, Shuji Suzuki, Derya Aytemiz, Toshiroh Iwasaki, Ryo 저
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- 3,000원
This study was conducted to assess the ability of two-dimensional tissue tracking (2DTT) to evaluate changes in left ventricular (LV) myocardial function associated with sustained high electrical pacing. Pacemakers were implanted at the right ventricular (RV) apex of five female Beagles, and sustained high electrical pacing of 250 beats per minute (bpm) was performed for three consecutive weeks. Conventional echocardiography and 2DTT were performed at baseline, and at every week for three weeks...
228.

The signal sequence of type II porcine reproductive and respiratory syndrome virus glycoprotein 3 is sufficient for endoplasmic reticulum retention

Do-Geun Kim, Chang-Seon Song, In-Soo Choi, Seung-Yong Park, Joong-Bok Lee, Sang-Soo Lee 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 10 307-313 (7 pages)

Do-Geun Kim, Chang-Seon Song, In-Soo Choi, Seung-Yong Park, Joong-Bok Lee, Sang-Soo Lee 저
...
- 3,000원
The glycoprotein 3 (GP3) of type II porcine reproductive and respiratory syndrome virus has the characteristic domains of a membrane protein. However, this protein has been reported to be retained in the endoplasmic reticulum (ER) rather than transported to the plasma membrane of the cell. In this study, we performed confocal laser scanning microscopy analysis of variants of GP3 and foundthat the signal sequence of the GP3 led to confinement of GP3 in the ER, while the functional ortransmembrane...
229.

Dissimilarity of ccrAB gene sequences between methicillin-resistant Staphylococcus epidermidis and methicillin-resistant Staphylococcus aureus among bovine isolates in Korea

Young Kyung Park, Young Hwan Paik, Jang Won Yoon, Lawrence K. Fox, Sun Young Hwang, Yong Ho Park 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 9 299-305 (7 pages)

Young Kyung Park, Young Hwan Paik, Jang Won Yoon, Lawrence K. Fox, Sun Young Hwang, Yong Ho Park 저
...
- 3,000원
The sequences of the ccrAB genes from bovine-, canine- and chicken-originating methicillin-resistant Staphylococcus (S.) epidermidis (MRSE) and bovine methicillin-resistant Staphylococcus (S.) aureus (MRSA) were compared to investigate the frequency of intra-species horizontal transfer of the staphylococcal cassette chromosome mec (SCCmec) complex. Nineteen MRSE strains were isolated from bovine milk, chickens, and dogs, and their genetic characteristics were investigated by multilocus sequence...
230.

Preparation and diagnostic utility of a hemagglutination inhibition test antigen derived from the baculovirus-expressed hemagglutinin-neuraminidase protein gene of Newcastle disease virus

Kang-Seuk Choi, Soo-Jeong Kye, Woo-Jin Jeon, Mi-Ja Park, Saeromi Kim, Hee-Jung Seul, Jun-Hun Kwon 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 8 291-297 (7 pages)

Kang-Seuk Choi, Soo-Jeong Kye, Woo-Jin Jeon, Mi-Ja Park, Saeromi Kim, Hee-Jung Seul, Jun-Hun Kwon 저
...
- 3,000원
A recombinant hemagglutinin-neuraminidase (rHN) protein from Newcastle disease virus (NDV) with hemagglutination (HA) activity was expressed in Spodoptera frugiperda cells using a baculovirus expression system. The rHN protein extracted from infected cells was used as an antigen in a hemagglutination inhibition (HI) test for the detection and titration of NDV-specific antibodies present in chicken sera. The rHN antigen produced high HA titers of 2 per 25 μL, which were similar to those of the...

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