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Development of Nested PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Cylindrocladium scoparium on Eucalyptus
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 5호 5 414-422 (9 pages)
nested PCR, multiplex PCR, loop-mediated isothermal amplification (LAMP) were developed for detection of C. scoparium based on factor 1-alpha (tef1) and beta-tubulin gene in this study. All of the three methods showed highly specific to C. scoparium. The sensitivities of the nested PCR and LAMP were much higher than the multiplex PCR. The sensitivity of multiplex PCR was also higher than regular PCR. C. scoparium could be detected within 60 min from infected Eucalyptus plants by LAMP, while at... -
Antifungal Effect of Chitosan as Ca2+ Channel Blocker
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 3호 8 242-250 (9 pages)
was found to show the highest antifungal activity against P. italicum. Furthermore, the effect of CO-F2 toward this fungus was significantly reduced in the presence of Ca2+, whereas its effect was recovered by ethylenediaminetetraacetic acid, suggesting that the CO-F2 acts via disruption of Ca2+ gradient required for survival of the fungus. Our results suggest that CO-F2 may serve as potential compounds to develop alternatives to synthetic fungicides for the control of the postharvest diseases. -
Development of a Rapid Detection Method for Potato virus X by Reverse Transcription Loop-Mediated Isothermal Amplification
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 3호 3 219-225 (7 pages)
is the development of simple, rapid, and sensitive virus detection. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been used to detect viral RNA molecules because of its simplicity and high sensitivity for a number of viruses. RT-LAMP for the detection of Potato virus X (PVX) was developed and compared with conventional reverse transcription polymerase chain reaction (RT-PCR) to demonstrate its advantages over RT-PCR. RT-LAMP reactions were conducted with or without a... -
Altered Cultivar Resistance of Kimchi Cabbage Seedlings Mediated by Salicylic Acid, Jasmonic Acid and Ethylene
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2014, 30권 3호 12 323-329 (7 pages)
acid (ACC) ethylene precursor did not change resistance of the either cultivar to C. higginsianum and Xcc. Effect of ACC pretreatment on the resistance to A. brassicicola was not distinguished between susceptible and resistant cultivars, because cultivar resistance of the resistant cultivar was lost by prolonged moist dark conditions. Taken together, exogenously applied SA, JA and ethylene altered defense signaling crosstalk to three diseases of anthracnose, black spot and black rot in a... -
Induced Systemic Drought and Salt Tolerance by Pseudomonas chlororaphis O6 Root Colonization is Mediated by ABA-independent Stomatal Closure
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2012, 28권 2호 10 202-206 (5 pages)
Root colonization by the rhizobacterium Pseudomonas chlororaphis O6 in Arabidopsis thaliana Col-0 plants resulted in induced tolerance to drought and salinity caused by halide salt-generated ionic stress but not by osmotic stress caused by sorbitol. Stomatal apertures decreased following root colonization by P. chlororaphis O6 in both wild-type and ABA-insensitive Arabidopsis mutant plants. These results suggest that an ABAindependent stomatal closure mechanism in the guard cells of P.... -
Research Articles : Effect of Rice stripe virus NS3 on Transient Gene Expression and Transgene Co-Silencing
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 4호 2 310-314 (5 pages)
in the absence of NS3. In addition, NS3 significantly suppressed the initiation of GFP co-silencing induced by the additive GFP infiltration in GFP-transgenic Nicotiana benthamiana. The NS3 gene was also found to be a stronger suppressor than Cucumber mosaic virus 2b. These observations are believed to be derived from the strong suppressive effect of NS3 on gene silencing, and indicate that NS3 could be used as an effective enhancer for the rapid production of foreign proteins in plants. -
Direct Evidence of Endophyte (Neotyphodium coenophialum) Genotype Effect on Growth and Vertical Transmission of Endophyte in Tall Fescue (Schedonorus phoenix Scop.) Under Water Stress
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 3호 6 249-256 (8 pages)
to examine the effect of endophyte genotype and water stress on endophyte transmission by imposing soil moisture deficits at different stages of panicle development. There was water stress effect on endophyte frequency but not on concentration, whereas endophyte genotype significantly influenced endophyte concentration in pseudostem of tall fescue at boot stage. Reproductive tillers showed greater endophyte frequency and concentration. Endophyte frequency in florets or seeds depended on position... -
Effect of Salinity, Temperature and Carbon Source on the Growth and Development of Sclerotia of Sclerotinia sclerotiorum Isolated from Semi-arid Environment
한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2008, 24권 4호 6 407-416 (10 pages)
sclerotia were significantly reduced. The combined effect of temperature, solute potential and carbon source on sclerotia production were highly significant and had an impact on the development of the rind layer cells of sclerotia. These cells lacked a transparent cell wall which was surrounded by a compact melanized layer, and some of these cells appeared to be devoid of cell contents or were totally vacuolated. The survival of the sclerotia with increase in salinity and temperature appeared to... -
COX-2 inhibitory effect of luteolin through the regulation of NR-κB and ERK in phorbol ester activated human lung epithelial cells
Chung Mu Park 인문사회과학기술융합학회 예술인문사회융합멀티미디어논문지 8 Pages
인문사회과학기술융합학회 예술인문사회융합멀티미디어논문지 2017, 제 7권 제 5호 42 467-474 (8 pages)
The inhibitory effect of luteolin on cyclooxygenase (COX)-2 expression was investigated in 12-O-tetradecanoyl phorbol-13-acetate (TPA), a prototype tumor promoter, activated human lung epithelial A549 cells. Luteolin mitigated TPA-stimulated COX-2 expression in a dose-dependent manner without any cytotoxicity. Since nuclear factor (NF)-κB plays a critical role in inflammatory casdades, activated status of NF-κB was estimated through the phosphorylation of p65, one subunit of NF-κB by western... -
Effect of Environmental Enrichment on Cognitive Impairment-induced by Ethanol Exposure in Adolescent Rat
Yoon Ju Kim, Jong Min Park, Min Kyung Song, Ho Hyun Seong, Youn Jung Kim 한국기초간호학회 Journal of Korean Biological Nursing Science 6 Pages
한국기초간호학회 Journal of Korean Biological Nursing Science 2016, Volume 18, No 4 10 274-279 (6 pages)
Purpose: Adolescents who experienced the alcohol consumption have gradually increased. Adolescence is a critical period of the neural plasticity in the brain. Neural plasticity is mediated by neurotrophins and has an impact on cognitive function. Environmental enrichment ameliorates the cognitive function and increases neurotrophins. Thus, we investigated the neuroprotective effect of environmental enrichment on ethanol induced cognitive impairment in adolescent rats. Methods: The ethanol groups...


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