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The association of Hsp90 expression induced by aspirin with anti-stress damage in chicken myocardial cells
Xiao-hui Zhang, Huai-sen Zhu, Zhuang Qian, Shu Tang, Di Wu, Nicole Kemper, Joerg Hartung, En-dong Bao 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 5 35-44 (10 pages)
of Hsp90 protein and hsp90 mRNA expression in chicken heart tissues after oral administration of aspirin following exposure to high temperature for varying times. Heat stress induced increases in plasma aspartate aminotransferase, creatine kinase and lactate dehydrogenase activities while causing severe heart damage, which was characterized by granular and vacuolar degeneration, nuclear shrinkage and even myocardium fragmentation in cardiac muscle fibers. After aspirin administration, myocardial... -
Hepatitis E virus as an emerging zoonotic pathogen
Woo-Jung Park, Byung-Joo Park, Hee-Seop Ahn, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Sang-Won Lee, Han-Sang Yoo, In-Soo 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 1 1-11 (11 pages)
Hepatitis E outbreaks are a serious public health concern in developing countries. The disease causes acute infections, primarily in young adults. The mortality rate is approximately 2%; however, it can exceed 20% in pregnant women in some regions in India. The causative agent, hepatitis E virus (HEV), has been isolated from several animal species, including pigs. HEV genotypes 3 and 4 have been isolated from both humans and animals, and are recognized as zoonotic pathogens. Seroprevalence... -
Isolation and phylogenetic analysis of hemagglutinin gene of H9N2 influenza viruses from chickens in South China from 2012 to 2013
Han-Qin Shen, Zhuan-Qiang Yan, Fan-Gui Zeng, Chang-Tao Liao, Qing-Feng Zhou, Jian-Ping Qin, Qing-Mei Xie, Ying-Zuo Bi, Feng Chen 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 9 317-324 (8 pages)
As part of our ongoing influenza surveillance program in South China, 19 field strains of H9N2 subtype avian influenza viruses (AIVs) were isolated from dead or diseased chicken flocks in Guangdong province, South China, between 2012 and 2013. Hemagglutinin (HA) genes of these strains were sequenced and analyzed and phylogenic analysis showed that 12 of the 19 isolates belonged to the lineage h9.4.2.5, while the other seven belonged to h9.4.2.6. Specifically, we found that all of the viruses... -
Establishment and evaluation of a murine ανβ3-integrin- expressing cell line with increased susceptibility to Foot- and-mouth disease virus
Wei Zhang, Kaiqi Lian, Fan Yang, Yang Yang, Zhijian Zhu, Zixiang Zhu, Weijun Cao, Ruoqing Mao, Ye Jin, Jijun He, Jianhong Guo, X 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 3 265-272 (8 pages)
cell line expressing the murine ανβ3 heterodimer (designated as “CHO-677-mανβ3” cells) was established using a highly efficient lentiviral-mediated gene transfer technique. Integrin subunits αν and β3 were detected at the gene and protein levels by polymerase chain reaction (PCR) and indirect immunofluorescent assay (IFA), respectively, in the CHO-677-mανβ3 cell line at the 20th passage, implying that these genes were successfully introduced into the CHO-677 cells and expressed... -
Cloning, expression and functional analysis of the duck Toll-like receptor 5 (TLR5) gene
Yuqiang Cheng, Yingjie Sun, Hengan Wang, Shuduan Shi, Yaxian Yan, Jing Li, Chan Ding, Jianhe Sun 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 1호 6 37-46 (10 pages)
expressed in the heart, liver, kidney, and muscle. DF-1 or HeLa cells transfected with DNA constructs encoding dTLR5 can activate NF-ĸB leading to the activation of interleukin-6 (IL-6) promoter. When we challenged ducks with a Herts33 Newcastle disease virus (NDV), mRNA transcription of the antiviral molecules Mx, Double stranded RNA activated protein kinase (PKR), and OAS was up-regulated in the liver, lung, and spleen 1 and 2 days post-inoculation. -
Assessing PCV2 antibodies in field pigs vaccinated with different porcine circovirus 2 vaccines using two commercial ELISA systems
Min-Kyoung Shin, Seung Hyun Yoon, Myung Hwui Kim, Young Soo Lyoo, Seung Won Suh, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 1호 4 25-29 (5 pages)
Porcine circovirus type 2 (PCV2) is the primary causative agent for post-weaning, multisystemic, wasting syndrome. Consequently, serologic detection of and vaccination against PCV2 are important for the swine industry. Among several serological tests, the enzyme-linked immunosorbent assay (ELISA) is commonly used to measure anti-PCV2 antibody levels. In the present study, we used two commercial ELISA systems to comparatively evaluate anti-PCV2 antibodies in field pigs... -
Use of laparoscopy for diagnosing experimentally induced acute pancreatitis in dogs
Hyun-wook Kim, Ye-in Oh, Ji-hye Choi, Dae-yong Kim, Hwa-young Youn, 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 16 551-556 (6 pages)
Diagnosis of acute pancreatitis in dogs remains a significant challenge despite the development of advanced diagnostic methodologies. Visual inspection and pancreas biopsy using laparoscopy are generally considered to be procedures free of complications when conducted on healthy animals. However, the usefulness of laparoscopy for diagnosing acute pancreatitis has not been assessed. In the present study, the efficacy of laparoscopy for diagnosing acute pancreatitis in dogs was evaluated in... -
Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus
Xiaoyu Deng, Yanlong Cong, Renfu Yin, Guilian Yang, Chan Ding, Shengqing Yu, Xiufan Liu, Chunfeng Wang, †, Zhuang Ding, 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 7 511-517 (7 pages)
Characteristic clinical manifestations of Newcastle disease include leukopenia and immunosuppression. Peripheral blood mononuclear cells (PBMCs) are the main targets of Newcastle disease virus (NDV) infection. To survey changes in proteomic expression in chicken PBMCs following NDV infection, PBMC proteins from 30 chickens were separated using two- dimensional electrophoresis (2-DE) and subjected to mass spectrometry analysis. Quantitative intensity analysis showed that the expression of 78... -
Equine hyperimmune serum protects mice against Clostridium difficile spore challenge
Weiwei Yan, Kang-Soon Shin, Shih-Jon Wang, Hua Xiang, Thomas Divers, Sean McDonough, James Bowman, Anne Rowlands, Bruce Akey, Hu 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 11 249-258 (10 pages)
Clostridium (C.) difficile is a common cause of nosocomial diarrhea in horses. Vancomycin and metronidazole have been used as standard treatments but are only moderately effective, which highlights the need for a novel alternative therapy. In the current study, we prepared antiserum of equine origin against both C. difficile toxins A and B as well as whole-cell bacteria. The toxin-neutralizing activities of the antibodies were evaluated in vitro and the prophylactic effects of in vivo passive... -
Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus
Lishan Lv, Xiaoming Li, Genmei Liu, Ran Li, Qiliang Liu, Huifang Shen, Wei Wang, Chunyi Xue, Yongchang Cao* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 6 209-216 (8 pages)
S1 protein to the cytoplasmic and transmembrane domains of NA protein of avian influenza H5N1 virus. The chimeric VLPs elicited significantly higher S1-specific antibody responses in intramuscularly immunized mice and chickens than inactivated IBV viruses. Furthermore, the chimeric VLPs induced significantly higher neutralization antibody levels than inactivated H120 virus in SPF chickens. Finally, the chimeric VLPs induced significantly higher IL-4 production in mice. These results demonstrate...


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