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Microtubule distribution in somatic cell nuclear transfer bovine embryos following control of nuclear remodeling type
Dae-Jin Kwon, Yu-Mi Lee, In-Sun Hwang, Choon-Keun Park, Boo-Keun Yang, Hee-Tae Cheong 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 1 93-101 (9 pages)
This study was conducted to evaluate the microtubule distribution following control of nuclear remodeling by treatment of bovine somatic cell nuclear transfer (SCNT) embryos with caffeine or roscovitine. Bovine somatic cells were fused to enucleated oocytes treated with either 5 mM caffeine or 150 mM roscovitine to control the type of nuclear remodeling. The proportion of embryos that underwent premature chromosome condensation (PCC) was increased by caffeine treatment but was reduced by... -
Comparison of the age-related porcine endogenous retrovirus (PERV) expression using duplex RT-PCR
Hyoung Joon Moon, Hye Kwon Kim, Seong Jun Park, Chul Seung Lee, Dae Sub Song, Bo Kyu Kang, Bong Kyun Park* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 4호 7 317-322 (6 pages)
simultaneously detecting PERV gag mRNA and porcine glyceraldehydes 3-phosphate dehydrogenase mRNA in one tube was established. To compare the age-related PERV expression patterns of the lung, liver, spleen, kidney, heart, and pancreas in commercial pigs, 20 pigs from four age groups (5 heads each in 10 days-, 40 days-, 70 days-, and 110 days-old, respectively) were used in this study. The expression patterns of PERV were statistically different among age groups in lung, liver, and kidney (ANOVA,... -
Charting the proteome of Cryptosporidium parvum sporozoites using sequence similarity-based BLAST searching
A.M.A.M.Z. Siddiki*, Jonathan M. Wastling 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 5 203-210 (8 pages)
Cryptosporidium (C.) spp. are important zoonotic parasites causing widespread diarrhoeal disease in man and animals. The recent release of the complete genome sequences for C. parvum and C. hominis has facilitated the comprehensive global proteome analysis of these opportunistic pathogens. The well-known approach for mass spectrometry (MS) based data analysis using the BLAST tool (MS BLAST) is a database search protocol for identifying unknown proteins by sequence similarity to homologous... -
Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells
Min-Soo Seo, Yun-Hyeok Jeong, Jeung-Ran Park, Sang-Bum Park, Kyoung-Hwan Rho, Hyung-Sik Kim, Kyung-Rok Yu, Seung-Hee Lee, Ji-Won 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 2 181-187 (7 pages)
characterized. In this study, we successfully isolated and characterized MSCs from the canine umbilical cord and its fetal blood. Canine MSCs (cMSCs) were grown in medium containing low glucose DMEM with 20% FBS. The cMSCs have stem cells expression patterns which are concerned with MSCs surface markers by fluorescence- activated cell sorter analysis. The cMSCs had multipotent abilities. In the neuronal differentiation study, the cMSCs expressed the neuronal markers glial fibrillary acidic prote... -
Immunohistochemical localization of galectin-3 in the granulomatous lesions of paratuberculosis-infected bovine intestine
Juyeon Lee, Changjong Moon, Jihoon Kim, Chanwoo Jung, Keun-Hwa Lee, Hong-Gu Joo, Meejung Ahn, Taekyun Shin* 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 1 177-180 (4 pages)
The presence of galectin-3 was immunohistochemically quantified in bovine intestines infected with paratuberculosis (Johne's disease) to determine whether galectin-3 was involved in the formation of granulation tissue associated with the disease. Mycobacterium avium subsp. paratuberculosis infection was histochemically confirmed using Ziehl- Neelsen staining and molecularly diagnosed through rpoB DNA sequencing. Galectin-3 was detected in the majority of inflammatory cells, possibly macrophages,... -
Genetic analysis of ORF5 of recent Korean porcine reproductive and respiratory syndrome viruses (PRRSVs) in viremic sera collected from MLV-vaccinating or non-vaccinating farms
Hye Kwon Kim, Jeong Sun Yang, Hyoung Joon Moon, Seong Jun Park, Yuzi Luo, Chul Seung Lee, Dae Sub Song, Bo Kyu Kang, Soo Kyung A 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 2호 5 121-130 (10 pages)
The 23 open reading frame (ORF) 5 sequences of Korean type II porcine reproductive and respiratory syndrome virus (PRRSV) were collected from viremic sera from the (modified live vaccine) MLV-vaccinating and non-vaccinating farms from 2007 to 2008. The samples were phylogenetically analyzed with previous ORF5 sequences, including type I Korean PRRSV, and previously reported or collected sequences from 1997 to 2008. A MN184-like subgroup of type II Korean PRRSV was newly identified in the viremic... -
Low dietary inorganic phosphate affects the lung growth of developing mice
Cheng-Xiong Xu, Hua Jin, Youn-Sun Chung, Ji-Young Shin, Soon-Kyung Hwang, Jung-Taek Kwon, Sung-Jin Park, Eun-Sun Lee, Arash Mina 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 2호 3 105-113 (9 pages)
0.5% Pi, low: 0.1% Pi) for 4 weeks. After 4 weeks of the diet, all the mice were sacrificed. Their lungs were harvested and analyzed by performing luciferase assay, Western blotting, kinase assay and immunohistochemistry. Our results demonstrate that low Pi affects the lungs of developing mice by disturbing protein translation, the cell cycle and the expression of fibroblast growth factor-2. These results suggest that optimally regulating Pi consumption may be important to maintain health. -
Synergistic effect of ERK inhibition on tetrandrine-induced apoptosis in A549 human lung carcinoma cells
Hyun Sun Cho, Seung Hee Chang, Youn Sun Chung, Ji Young Shin, Sung Jin Park, Eun Sun Lee, Soon Kyung Hwang, Jung Taek Kwon, Aras 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 1호 4 23-28 (6 pages)
Tetrandrine (TET), a bis-benzylisoquinoline alkaloid from the root of Stephania tetrandra, is known to have anti-tumor activity in various malignant neoplasms. However, the precise mechanism by which TET inhibits tumor cell growth remains to be elucidated. The present studies were performed to characterize the potential effects of TET on phosphoinositide 3-kinase/Akt and extracellular signal-regulated kinase (ERK) pathways since these signaling pathways are known to be responsible for cell... -
Circadian variations in salivary chromogranin a concentrations during a 24-hour period in dogs
Kazutaka Kanai, Mariko Hino, Yasutomo Hori, Ruriko Nakao, Fumio Hoshi, Naoyuki Itoh, Seiichi Higuchi* 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 4호 13 421-424 (4 pages)
The purpose of this study was to determine if salivary chromogranin a secretion in dogs exhibits a circadian rhythm. Saliva sampling was performed during three different sessions occurring in three nonconsecutive 24-h periods. Sixteen healthy adult beagle dogs (8 males and 8 females) were moved to a sampling room and housed individually in cages. Saliva samples were obtained every 4 h from 12:00 p.m. to 12:00 p.m. the following day. In the interest of habituation, saliva was obtained hourly from... -
Implantation of canine umbilical cord blood-derived mesenchymal stem cells mixed with beta-tricalcium phosphate enhances osteogenesis in bone defect model dogs
Byung-Jun Jang, Ye-Eun Byeon, Ji-Hey Lim, Hak-Hyun Ryu, Wan Hee Kim, Yoshihisa Koyama, Masanori Kikuchi, Kyung-Sun Kang*, Oh-Kye 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 4호 8 387-394 (8 pages)
This study was performed to evaluate the osteogenic effect of allogenic canine umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) mixed with beta-tricalcium phosphate (b-TCP) in orthotopic implantation. Seven hundred milligrams of b-TCP mixed with 1 × 10 UCB-MSCs diluted with 0.5 ml of saline (group CM) and mixed with the same volume of saline as control (group C) were implanted into a 1.5 cm diaphyseal defect and wrapped with PLGC membrane in the radius of Beagle dogs....


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