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221.

Changes in orexin-A and neuropeptide Y expression in the hypothalamus of the fasted and high-fat diet fed rats

Eun Sung Park, Seong Joon Yi, Jin Sang Kim, Heungshik S. Lee, In Se Lee, Je Kyung Seong, Hee Kyung Jin, Yeo Sung Yoon 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 3 295-302 (8 pages)

Eun Sung Park, Seong Joon Yi, Jin Sang Kim, Heungshik S. Lee, In Se Lee, Je Kyung Seong, Hee Kyung Jin, Yeo Sung Yoon 저
...
- 3,000원
This study was aimed to investigate the changes of orexin-A (OXA) and neuropeptide Y (NPY) expression in the hypothalamus of the fasted and high-fat diet fed rats. For the experiments, the male Sprague-Dawley (SD) rats were used as the model of high-fat diet-induced obesity. The mean loss of body weight (MLBW) did not show the linear pattern during the fasting; from 24 h to 84 h of fastings, the MLBW was not significantly changed. The numbers of OXA-immunoreactive (IR) neurons were decreased at...
222.

Glucocorticoid-induced laminitis with hepatopathy in a Thoroughbred filly

Seung-ho Ryu, Byung-sun Kim, Chang-woo Lee, Junghee Yoon, Yonghoon Lyon Lee 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 3호 15 271-274 (4 pages)

Seung-ho Ryu, Byung-sun Kim, Chang-woo Lee, Junghee Yoon, Yonghoon Lyon Lee 저
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- 1,500원
A 3-year-old Thoroughbred filly was referred to the Equine Hospital, Korea Racing Association for evaluation of hematuria, inappetite, weight loss and depression. From 25 days prior to admission, the horse was treated for right carpal lameness with 20 mg intramuscular administration of triamcinolone acetonide per day for consecutive 10 days by a local veterinarian. Clinical and laboratory findings included vaginal hyperemia, flare in bladder wall, neutrophilia, lymphopenia, polyuria, polydipsia...
223.

Effect of β -mercaptoethanol or epidermal growth factor supplementation on in vitro maturation of canine oocytes collected from dogs with different stages of the estrus cycle

Min Kyu Kim, Yuda Heru Fibrianto, Hyun Ju Oh, Goo Jang, Hye Jin Kim, Kyu Seung Lee, Sung Keun Kang, Byeong Chun Lee, Woo Suk Hwa 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 3호 11 253-258 (6 pages)

Min Kyu Kim, Yuda Heru Fibrianto, Hyun Ju Oh, Goo Jang, Hye Jin Kim, Kyu Seung Lee, Sung Keun Kang, Byeong Chun Lee, Woo Suk Hwa 저
...
- 3,000원
Supplementation of β-mercaptoethanol (β-ME) in in vitro maturation (IVM) medium was shown to improve embryo development and quality in several species. Epidermal growth factor (EGF) was also shown to improve IVM of human oocyte and embryo development after in vitro fertilization (IVF). The effect of these two compounds were suggested to be mediated through the synthesis of glutathione (GSH) which is known to play an important role in protecting the cell or embryos from oxidative damage. Thus,...
224.

Exposure to genistein does not adversely affect the reproductive system in adult male mice adapted to a soy-based commercial diet

Beom Jun Lee, Jong-Koo Kang, Eun-Yong Jung, Young Won Yun, In-Jeoung Baek, Jung-Min Yon, Yoon-Bok Lee, Heon-Soo Sohn, Jae-Yong L 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 3호 7 227-234 (8 pages)

Beom Jun Lee, Jong-Koo Kang, Eun-Yong Jung, Young Won Yun, In-Jeoung Baek, Jung-Min Yon, Yoon-Bok Lee, Heon-Soo Sohn, Jae-Yong L 저
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- 3,000원
Genistein, a soybean-originated isoflavone, is widely consumed by humans for putative beneficial health effects but its estrogenic activity may affect adversely the development of male reproductive system. Five-week-old ICR mice were purchased and fed with a soybean-based Purina Chow diet until 6 months of age. The animals were exposed by gavage to genistein (2.5 mg/kg/day) or 17β-estradiol (7.5μg/kg/day) for five weeks. Corn oil was used for the negative control. The animals were fed the...
225.

Two-dimensional gel electrophoresis and immunoblot analysis of Neospora caninum tachyzoites

Eung-Goo Lee, Jae-Hoon Kim, Yong-Seung Shin, Gee-Wook Shin, Yong-Hwan Kim, Gon-Sup Kim, Dae-Yong Kim, Tae-Sung Jung, Myung-Deuk 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 8 139-145 (7 pages)

Eung-Goo Lee, Jae-Hoon Kim, Yong-Seung Shin, Gee-Wook Shin, Yong-Hwan Kim, Gon-Sup Kim, Dae-Yong Kim, Tae-Sung Jung, Myung-Deuk 저
...
- 3,000원
Identification of expressed protein profiles and antigenic determination are some of the most challenging aspects of proteomics. Two-dimensional gel electrophoresis (2-DE) combined with immunoblot analysis were employed to study the N. caninum proteome. Protein sample preparation was carried out by first conducting sonication, followed by adding lysis buffer containing 7M urea plus 2M thiourea to the purified tachyzoites in order to complete disruption. A total of 335 differentially expressed...
226.

B6C3F1 mice exposed to ozone with 4-(N-methyl-Nnitrosamino)-1-(3-pyridyl)-1-butanone and/or dibutyl phthalate showed toxicities through alterations of NF-κB, AP-1, Nrf2, and osteopontin

Min Young Kim, Kyung Suk Song, Gun Ho Park, Seung Hee Chang, Hyun Woo Kim, Jin Hong Park, Hwa Jin, Kook Jong Eu, Hyun Sun Cho, G 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 7 131-137 (7 pages)

Min Young Kim, Kyung Suk Song, Gun Ho Park, Seung Hee Chang, Hyun Woo Kim, Jin Hong Park, Hwa Jin, Kook Jong Eu, Hyun Sun Cho, G 저
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- 3,000원
Toxic effects of ozone, 4-(N-methyl-N-nitrosamino)-1-(3- pyridyl)-1-butanone (NNK), and/or dibutyl phthalate (DBP) were examined through NF-kB, AP-1, Nrf2, and osteopontin (OPN) in lungs and livers of B6C3F1 mice. Electrophoretic mobility shift assay (EMSA) indicated that mice treated with combination of toxicants induced high NF-kB activities. Expression levels of p105, p65, and p50 proteins increased in all treated mice, whereas IkB activity was inhibited in NNK-, DBP-, and combination-treated...
227.

Escherichia coli O157:H7 adherence to HEp-2 cells is implicated with curli expression and outer membrane integrity

Sang-Hyun Kim, Yong-Hwan Kim 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 5 119-124 (6 pages)

Sang-Hyun Kim, Yong-Hwan Kim 저
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- 3,000원
Escherichia coli (E. coli) has ability to express thin aggregative fimbriae, known as curli, on the cell surface. Previously, a few example of curli expression in serogroup O157:H7 of enterohemorrhagic E. coli (EHEC) were reported, compared to other E. coli groups. However, significance of curliation in the EHEC pathobiology has not been described well in the literature. A highly curliated O157:H7 strain was used in this study in order to elucidate role of curliation in EHEC adherence to...
228.

Optimization of in situ hybridization assay using nonradioactive DNA probes for the detection of canine erpesvirus (CHV) in paraffin-embedded sections

Okjin Kim 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 1호 10 71-73 (3 pages)

Okjin Kim 저
...
- 1,500원
Two non-radioactive probes using digoxigenin or biotin were developed for detecting canine herpesvirus (CHV) and compared for their sensitivities by in situ hybridization (ISH) in formalin fixed, paraffin embedded sections, which has been used routinely in veterinary fields. Sections of the CHV-infected cell preparation were subjected to several different ISH protocols using digoxigenin- or biotin-labeled probe respectively. Results were compared for the hybridization and background signal...
229.

Effects of Protein Source and Energy Substrates on the In Vitro Development of Bovine Embryos in a Two-step Culture System

Kwang-taek Lim, Byeong-chun Lee, Sung-keun Kang, Woo-suk Hwang 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 1호 11 73-78 (6 pages)

Kwang-taek Lim, Byeong-chun Lee, Sung-keun Kang, Woo-suk Hwang 저
...
- 3,000원
In this study, we examined the effects of a two-step culture system, which involves the use of different culture media for early cleavage and later stage embryos, on the in vitro development of bovine embryos. We also investigated the effect of glucose, phosphate and citrate on the in vitro early developmental period of bovine embryos in a two-step culture system. Moreover, the supplementation of different protein sources (BSA-V, BSA-FAF and FBS) during IVC did not affect the frequency of...
230.

Histochemical Characterization of the Lectin-binding Sites in the Equine Vomeronasal Organ

Jee-young Lee, Tae-young Kang, Yong-duk Lee, Tae-kyun Shin 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 1호 3 15-19 (5 pages)

Jee-young Lee, Tae-young Kang, Yong-duk Lee, Tae-kyun Shin 저
...
- 1,500원
The binding specificities of various lectins, such as the Dolichos biflorus agglutinin (DBA), soybean agglutinin (SBA), and the Bandeiraea simplicifolia BS-1 (Isolectin B4), Triticum vulgaris (WGA), Arachis hypogaea (PNA), and Ulex europaeus (UEA-I) lectins, were studied in the vomeronasal organ of the horse. The microvilli of the vomeronasal sensory epithelium were positive for DBA, SBA, Isolectin B4, WGA, PNA, and UEA-I. The receptor cells showed intense reactivity for DBA and WGA. Lectins...

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