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Production of cloned sei whale (Balaenoptera borealis) embryos by interspecies somatic cell nuclear transfer using enucleated pig oocytes
Eunsong Lee, Mohammad Musharraf Uddin Bhuiyan, Hiroyuki Watanabe, Kohji Matsuoka, Yoshihiro Fujise, Hajime Ishikawa, Yutaka Fuku 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 4호 2 285-292 (8 pages)
In this study, we examined the feasibility of using subzonal cell injection with electrofusion for interspecies somatic cell nuclear transfer (iSCNT) to produce sei whale embryos and to improve their developmental capacity by investigating the effect of osmolarity and macromolecules in the culture medium on the in vitro developmental capacity. Hybrid embryos produced by the electrofusion of fetal whale fibroblasts with enucleated porcine oocytes were cultured in modified porcine zygote medium-3... -
Effect of multi-planar CT image reformatting on surgeon diagnostic performance for localizing thoracolumbar disc extrusions in dogs
Jason B. King, Jeryl C. Jones, John H. Rossmeisl Jr*, Tisha A. Harper, Otto I Lanz, Stephen R. Werre 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 8 225-232 (8 pages)
Accurate pre-operative localization and removal of disc material are important for minimizing morbidity in dogs with thoracolumbar disc extrusions. Computed tomography (CT) is an established technique for localizing disc extrusions in dogs, however the effect of multi-planar reformatting (MPR) on surgeon diagnostic performance has not been previously described. The purpose of this study was to test the effect of MPR CT on surgeon diagnostic accuracy, certainty and agreement for localizing... -
Stereolithographic biomodeling of equine ovary based on 3D serial digitizing device
Junpei Kimura*, Nobunori Kakusho, Kenji Yamazawa, Yuuko Hirano, Yasuo Nambo, Hideo Yokota, Ryutaro Himeno 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 2호 11 161-163 (3 pages)
The 3D internal structure microscopy (3D-ISM) was applied to the equine ovary, which possesses peculiar structural characteristics. Stereolithography was applied to make a life-sized model by means of data obtained from 3D-ISM. Images from serially sliced surfaces contributed to a successful 3D reconstruction of the equine ovary. Photopolymerized resin models of equine ovaries produced by stereolithography can clearly show the internal structure and spatial localizations in the ovary. The... -
Genetic variability of the prion protein gene (PRNP) in wild ruminants from Italy and Scotland
Simone Peletto*, Matteo Perucchini, Cristina Acín, Mark P. Dalgleish, Hugh W. Reid, Roberto Rasero, Paola Sacchi, Paula Ste 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 2호 4 115-120 (6 pages)
(SNPs). In red deer, SNPs were found in codons 15, 21, 59, 78, 79, 98, 136, 168 and 226. These polymorphisms give rise to 12 haplotypes, and one of which is identical to the PRNP of American wapiti (Rocky Mountain elk, Cervus elaphus nelsoni). One silent mutation at codon 119 was detected in chamois and no SNPs were found in roe deer. This analysis confirmed that European wild ruminants have a PRNP genetic background that is compatible with TSE susceptibility, including chronic wasting disease. -
The role of Bcl-xL and nuclear factor-kB in the effect of taxol on the viability of dendritic cells
Mi-Hyoung Kim, Hong-Gu Joo* 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 2호 2 99-103 (5 pages)
Taxol has been used effectively in cancer therapies. Our previous study demonstrated that taxol induced altered maturation and improved viability of dendritic cells (DCs). However, the effects of taxol on DC viability have not been fully elucidated. In the present study, flow cytometric analyses revealed that taxol treatment significantly increased the number of viable DCs and the expression levels of a representative anti-apoptotic protein Bcl-xL. Furthermore, mobilization of the p65 subunit of... -
Imaging evaluation of the liver using multi-detector row computed tomography in micropigs as potential living liver donors
Jung Min Ryu, Dong Hyun Kim, Min Young Lee, Sang Hun Lee, Jae Hong Park, Seung Pil Yun, Min Woo Jang, Seong Hwan Kim, Gyu Jin Rh 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 2호 1 93-98 (6 pages)
The shortage of organ donors has stimulated interest in the possibility of using animal organs for transplantation into humans. In addition, pigs are now considered to be the most likely source animals for human xenotransplantation because of their advantages over non-human primates. However, the appropriate standard values for estimations of the liver of micropigs have not been established. The determination of standard values for the micropig liver using multi-detector row computed tomography... -
Cytokine response in Balb/c mice infected with Francisellatularensis LVS and the Pohang isolate
Eun-Ju Kim, Sang-Hee Park, Young-Sill Choi, Soo-Kyoung Shim, Mi-Yeoun Park, Man Suck Park, Kyu-Jam Hwang 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 3호 12 309-316 (8 pages)
after infection. Using these samples, RT-PCR and ELISA analysis were carried out for the comparative study of the cytokines, including TNF-a, INF-g, IL-2, IL-4, IL-10 and IL-12. In the Pohang-infected mice at 120 h, the liver showed a 53 times higher level of TNF-a and a 42 times higher level of IFN-g than the respective levels at the early time points after infection. The levels of TNF-a and IFN-g induced by LVS were 5 times lower than those induced by the Pohang isolate. Also, the organs from... -
The C-terminal variable domain of LigB from Leptospira mediates binding to fibronectin
Yi-Pin Lin, Yung-Fu Chang* 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 2호 3 133-144 (12 pages)
Adhesion through microbial surface components that recognize adhesive matrix molecules is an essential step in infection for most pathogenic bacteria. In this study, we report that LigB interacts with fibronectin (Fn) through its variable region. A possible role for LigB in bacterial attachment to host cells during the course of infection is supported by the following observations: (i) binding of the variable region of LigB to Madin-Darby canine kidney (MDCK) cells in a dose-dependent manner... -
Comparison of cardiac function and coronary angiography between conventional pigs and micropigs as measured by multidetector row computed tomography
Young Keun Ahn, Jung Min Ryu, Hea Chang Jeong, Yun Hyeon Kim, Myung Ho Jeong, Min Young Lee, Sang Hun Lee, Jae Hong Park, Seung 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 2호 1 121-126 (6 pages)
of micropigs had not been established. Computed tomography (CT) analysis aimed at estimating cardiac function and assessing the coronary arteries has not been carried out in micropigs. This study determined the feasibility of evaluating cardiac function in a micropig model using multidetector row computed tomography (MDCT) and compared the cardiac function values with those of conventional pigs. The mean age of the conventional pigs and micropigs was approximately 80 days and approximately 360... -
Changes of biomarkers with oral exposure to benzo(a)pyrene, phenanthrene and pyrene in rats
Hwan Goo Kang, Sang Hee Jeong*, Myung Haing Cho, Joon Hyoung Cho 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 4호 8 361-368 (8 pages)
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental contaminants present in air and food. Among PAHs, benzo(a)pyrene(BaP), phenanthrene (PH) and pyrene (PY) are considered to be important for their toxicity or abundance. To investigate the changes of biomarkers after PAH exposure, rats were treated with BaP (150 ■g/kg) alone or with PH (4,300 ■g/kg) and PY (2,700 ■g/kg) (BPP group) by oral gavage once per day for 30 days. 7-ethoxyresorufin-O-deethylase activity in liver...


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