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231.

Improved assessment of frozen/thawed mouse spermatozoa using fluorescence microscopy

Ann-Kathrin Diercks, Heinrich F. B?rgers, Anna Schwab, Johannes Schenkel* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 14 315-322 (8 pages)

Ann-Kathrin Diercks, Heinrich F. B?rgers, Anna Schwab, Johannes Schenkel* 저
...
- 3,000원
Genetically modified (GM) animals are unique mutants with an enormous scientific potential. Cryopreservation of pre-implantation embryos or spermatozoa is a common approach for protecting these lines from being lost or to store them in a repository. A mutant line can be taken out of a breeding nucleus only if sufficient numbers of samples with an appropriate level of quality are cryopreserved. The quality of different donors within the same mouse line might be heterogeneous and the...
232.

Protective effects of recombinant Brucella abortus Omp28 against infection with a virulent strain of Brucella abortus 544 in mice

Jeong Ju Lim, Dong Hyeok Kim, Jin Ju Lee, Dae Geun Kim, Wongi Min, Hu Jang Lee, Man Hee Rhee, Suk Kim* 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 10 287-292 (6 pages)

Jeong Ju Lim, Dong Hyeok Kim, Jin Ju Lee, Dae Geun Kim, Wongi Min, Hu Jang Lee, Man Hee Rhee, Suk Kim* 저
...
- 3,000원
The outer membrane proteins (OMPs) of Brucella (B.) abortus have been extensively studied, but their immunogenicity and protective ability against B. abortus infection are still unclear. In the present study, B. abortus Omp28, a group 3 antigen, was amplified by PCR and cloned into a maltose fusion protein expression system. Recombinant Omp28 (rOmp28) was expressed in Escherichia coli and was then purified. Immunogenicity of rOmp28 was confirmed by Western blot analysis with Brucella-positive...
233.

Prevalence of peste des petits ruminants among sheep and goats in India

Vinayagamurthy Balamurugan, Paramasivam Saravanan, Arnab Sen, Kaushal Kishor Rajak, Gnanavel Venkatesan, Paramanandham Krishnamo 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 9 279-285 (7 pages)

Vinayagamurthy Balamurugan, Paramasivam Saravanan, Arnab Sen, Kaushal Kishor Rajak, Gnanavel Venkatesan, Paramanandham Krishnamo 저
...
- 3,000원
This study measured the clinical prevalence of peste des petits ruminants (PPR) among sheep and goats in India between 2003 and 2009 by analyzing clinical samples from suspected cases of PPR that were submitted to the Rinderpest and Allied Disease Laboratory, Division of Virology, IVRI, Mukteswar for PPR diagnosis. PPR outbreaks were confirmed by detecting PPR virus (PPRV)-specific antigen in the clinical samples. Clinical samples (blood, nasal swabs, spleen, lymph node, kidney, liver,...
234.

Frequency of group A rotavirus with mixed G and P genotypes in bovines: predominance of G3 genotype and its emergence in combination with G8/G10 types

Yashpal S. Malik*, Kuldeep Sharma, Nirupama Vaid, Somendu Chakravarti, K. M. Chandrashekar, Sanjay S. Basera, Rashmi Singh, Mina 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 8 271-278 (8 pages)

Yashpal S. Malik*, Kuldeep Sharma, Nirupama Vaid, Somendu Chakravarti, K. M. Chandrashekar, Sanjay S. Basera, Rashmi Singh, Mina 저
...
- 3,000원
The present study describes the genotypic distribution of rotaviruses (RVs) in an Indian bovine population with unexpectedly higher proportions of G3 alone or in combination of G8/G10. PCR-genotyping confirmed that 39.4% (13/33) of the prevalent RVs were the G3 type while 60.6% (20/33) were dual G3G10 or G3G8 types. P typing revealed that 93.9% (31/33) of the samples were P[11] while 6.1% (2/33) possessed a dual P[1]P[11] type. Sequence analysis of the VP7 gene from G3 strains viz. B-46, 0970,...
235.

Hsp70 and HSF-1 expression is altered in the tissues of pigs transported for various periods of times

Miao Zhang, Zhenhua Yue, Zhijun Liu, Ali Islam, Buriro Rehana, Shu Tang, Endong Bao*, Jorg Hartung 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 6 253-259 (7 pages)

Miao Zhang, Zhenhua Yue, Zhijun Liu, Ali Islam, Buriro Rehana, Shu Tang, Endong Bao*, Jorg Hartung 저
...
- 3,000원
The aim of this study was to assess changes of Hsp70 and HSF-1 protein and mRNA expression in stress-sensitive organs of pigs during transportation for various periods of time. Twenty pigs were randomly divided into four groups (0 h, 1 h, 2 h, and 4 h of transportation). A significant increased activity of AST and CK was observed after 1 h and 2 h of transportation. Histopathological changes in the heart, liver, and stomach indicated that these organs sustained different degrees of injury. Hsp70...
236.

Evaluation of modified vaccinia virus Ankara expressing VP2 protein of infectious bursal disease virus as an immunogen in chickens

Flavia Adriana Zanetti*, Mar?a Paula Del M?dico Zajac, Oscar Alberto Taboga, Gabriela Calamante 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 12 199-201 (3 pages)

Flavia Adriana Zanetti*, Mar?a Paula Del M?dico Zajac, Oscar Alberto Taboga, Gabriela Calamante 저
...
- 1,500원
A recombinant modified vaccinia Ankara (MVA) virus expressing mature viral protein 2 (VP2) of the infectious bursal disease virus (IBDV) was constructed to develop MVA-based vaccines for poultry. We demonstrated that this recombinant virus was able to induce a specific immune response by observing the production of anti-IBDV-seroneutralizing antibodies in specific pathogen-free chickens. Besides, as the epitopes of VP2 responsible to induce IBDV-neutralizing antibodies are discontinuous, our...
237.

The role of inflammation and matrix metalloproteinases in equine endometriosis

Luca Aresu*, Silvia Benali, Diana Giannuzzi, Roberto Mantovani, Massimo Castagnaro, Maria Elena Falomo 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 8 171-177 (7 pages)

Luca Aresu*, Silvia Benali, Diana Giannuzzi, Roberto Mantovani, Massimo Castagnaro, Maria Elena Falomo 저
...
- 3,000원
Equine endometriosis is a multifactorial disease considered to be a major cause of equine infertility. The purpose of this study was to evaluate the reliability of histomorphological grading for biopsy-like samples compared to entire uterine wall samples, to examine the association between the degree of endometriosis with animal age, and to investigate the role of inflammation in endometriosis and the expression of different matrix metalloproteinases in equine endometrium. Histomorphological...
238.

In vitro susceptibility of Staphylococcus aureus strains isolated from cows with subclinical mastitis to different antimicrobial agents

Ayman El Behiry*, Gerd Schlenker, Istvan Szabo, Uwe Roesler 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 6 153-161 (9 pages)

Ayman El Behiry*, Gerd Schlenker, Istvan Szabo, Uwe Roesler 저
...
- 3,000원
Sensitivity to commercial teat dips (nonoxinol-9 iodine complex and chlorhexidine digluconate) of 56 Staphylococcus (S.) aureus strains isolated from quarter milk samples of various German dairy herds treated with different teat dipping schemes was investigated in this study. The minimum inhibitory concentration was determined using a broth macrodilution method according to the German Veterinary Association guidelines. The main objective of the current study was to induce in vitro resistance...
239.

Production of a highly group-specific monoclonal antibody against zearalenone and its application in an enzyme-linked immunosorbent assay

Sang-Ho Cha, Sung-Hee Kim, Karyn Bischoff, Hyun-Jeong Kim, Seong-Wan Son, Hwan-Goo Kang* 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 2 119-125 (7 pages)

Sang-Ho Cha, Sung-Hee Kim, Karyn Bischoff, Hyun-Jeong Kim, Seong-Wan Son, Hwan-Goo Kang* 저
...
- 3,000원
A monoclonal antibody (mAb) against zearalenone (ZEN) was produced using ZEN-carboxymethoxylamine and -BSA conjugates. Antibody produced by one clone showing a very high binding ability was selected and found to have a higher affinity for ZEN compared to a commerciall ZEN antibody. We developed two direct competitive ELISA systems using the selected antibody (ZEN-coated and anti-ZEN antibody-coated ELISA). Quantitative ranges for the anti-ZEN antibody-coated ELISA and ZEN-coated ELISA were from...
240.

Isolation and genetic characterization of Japanese encephalitis virus from equines in India

Baldev R. Gulati*, Harisankar Singha, Birendra K. Singh, Nitin Virmani, Sanjay Kumar, Raj K. Singh 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 1 111-118 (8 pages)

Baldev R. Gulati*, Harisankar Singha, Birendra K. Singh, Nitin Virmani, Sanjay Kumar, Raj K. Singh 저
...
- 3,000원
Japanese encephalitis (JE) is an important vector-borne viral disease of humans and horses in Asia. JE outbreaks occur regularly amongst humans in certain parts of India and sporadic cases occur among horses. In this study, JE seroprevalence and evidence of JE virus (JEV) infection among horses in Haryana (India) is described. Antibodies against JEV were detected in 67 out of 637 (10.5%) horses screened between 2006 and 2010. Two foals exhibiting neurological signs were positive for JEV RNA by...

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