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The combination of deoxynivalenol and zearalenone at permitted feed concentrations causes serious physiological effects in young pigs
Feng Chen, Yulin Ma, Chunyi Xue, Jingyun Ma, Qingmei Xie, Genhu Wang, Yingzuo Bi, Yongchang Cao, * 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 1호 6 39-44 (6 pages)
This study was to investigate the effects of the combination of deoxynivalenol (DON) and zearalenone (ZON) on pigs. Twenty-four weaning piglets were divided into a control group fed a diet free of mycotoxins and a toxin group fed a diet containing 1 mg/kg DON and 250 Ռg/kg ZON. The results showed that supplementation of DON and ZON in diets had extensive effects on pigs. More specifically, DON and ZON caused levels of total protein, albumin, and globulin in sera to decrease (p < 0.05) by... -
Enhanced tyrosine hydroxylase expression in PC12 cells co-cultured with feline mesenchymal stem cells
Guang-Zhen Jin, Xi-Jun Yin, Xian-Feng Yu, Su-Jin Cho, Hyo-Sang Lee, Hyo-Jong Lee, Il-Keun Kong* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 4호 10 377-382 (6 pages)
Mesenchymal stem cells (MSCs) secrete a variety of neuroregulatory molecules, such as nerve growth factor, brain-derived neurotrophic factor, and glial cell-derived neurotrophic factor, which upregulate tyrosine hydroxylase (TH) gene expression in PC12 cells. Enhancing TH gene expression is a critical step for treatment of Parkinson's disease (PD). The objective of this study was to assess the effects of co-culturing PC12 cells with MSCs from feline bone marrow on TH protein expression. We... -
Effects of endocrine disrupting chemicals on expression of phospholipid hydroperoxide glutathione peroxidase mRNA in rat testes
In-Jeoung Baek, Jung-Min Yon, Se-Ra Lee, Yan Jin, Mi-Ra Kim, Byeongwoo Ahn, Jin Tae Hong, Young-Kug Choo, Beom Jun Lee, Young Wo 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 3호 2 213-218 (6 pages)
Phospholipid hydroperoxide glutathione peroxidase (PHGPx), an antioxidative selenoprotein, is modulated by estrogen in the testis and oviduct. To examine whether potential endocrine disrupting chemicals (EDCs) affect the microenvironment of the testes, the expression patterns of PHGPx mRNA and histological changes were analyzed in 5-week-old Sprague-Dawley male rats exposed to several EDCs such as an androgenic compound [testosterone (50, 200, and 1,000 ?g/kg)], anti-androgenic compounds... -
Biological characteristics of Chinese hamster ovary cells transfected with bovine Prnp
Sang-Gyun Kang, Deog-Yong Lee, Mi Lan Kang, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 5 131-137 (7 pages)
A normal prion protein (PrPc) is converted to a proteaseresistant isoform by an apparent self-propagating activity in transmissible spongiform encephalopathy, a neurodegenerative disease. The cDNA encoding open reading frame (ORF) of the bovine prion protein gene (Prnp) was cloned from Korean cattle by PCR, and was transfected into Chinese hamster ovary (CHO-K1) cells using lipofectamine. The gene expression of the cloned cDNA was confirmed by RT-PCR and Western blotting with the monoclonal... -
Radioprotective effects of an acidic polysaccharide of Panax ginseng on bone marrow cells
Hyun-Ji Kim, Mi-Hyoung Kim, Yun-Young Byon, Jae Woo Park, Youngheun Jee, Hong-Gu Joo 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 6 39-44 (6 pages)
An acidic polysaccharide of Panax ginseng (APG), so called ginsan is known to have important immunomodulatory activities. It was recently reported that APG has radioprotective effects in mice but the detailed mechanism was not fully elucidated. This study examined the effects of APG on bone marrow cells (BMs). The phenotypical and functional changes in APG-treated BMs after gamma radiation were studied. The benefit of APG on BMs damaged by gamma radiation was determined by measuring the cell... -
Estrogen receptor independent neurotoxic mechanism of bisphenol A, an environmental estrogen
Yoot Mo Lee, Min Jae Seong, Jae Woong Lee, Yong Kyung Lee, Tae Myoung Kim, Sang-Yoon Nam, Dae Joong Kim, Young Won Yun, Tae Seon 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 5 27-38 (12 pages)
Bisphenol A (BPA), a ubiquitous environmental contaminant, has been shown to cause developmental toxicity and carcinogenic effects. BPA may have physiological activity through estrogen receptor (ER) -α and -β, which are expressed in the central nervous system. We previously found that exposure of BPA to immature mice resulted in behavioral alternation, suggesting that overexposure of BPA could be neurotoxic. In this study, we further investigated the molecular neurotoxic mechanisms of BPA. BPA... -
Detection of betanodaviruses in apparently healthy aquarium fishes and invertebrates
Dennis Kaw Gomez, Dong Joo Lim, Gun Wook Baeck, Hee Jeong Youn, Nam Shik Shin, Hwa Young Youn, Cheol Yong Hwang, Jun Hong Park, 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 11 369-374 (6 pages)
Betanodaviruses are the causative agents of viral nervous necrosis (VNN) in cultured marine fish. A total of 237 apparently healthy aquarium fish, marine (65 species) and freshwater (12 species) fishes and marine invertebrates (4 species), which were stocked in a commercial aquarium in Seoul, South Korea, were collected from November 2005 to February 2006. The brains of the fish and other tissues of the invertebrates were examined by reverse transcriptasepolymerase chain reaction (RT-PCR) and... -
Effects of DDA, CpG-ODN, and plasmid-encoded chicken IFN-γ on protective immunity by a DNA vaccine against IBDV in chickens
Ha Jung Roh, Haan Woo Sung, Hyuk Moo Kwon 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 10 361-368 (8 pages)
This study examined the adjuvant effects of dimethyl dioctadecyl ammonium bromide (DDA), CpG oligodeoxynucleotides (CpG-ODN), and chicken interferon-γ (ChIFN-γ) on a DNA vaccine (pcDNA-VP243) against the infectious bursal disease virus (IBDV). A plasmid encoding chicken IFN-a was constructed. Twice at 2-week intervals, twoweek- old chickens were injected intramuscularly and intraperitoneally with either a DNA vaccine alone or a DNA vaccine together with the respective adjuvants. On week 2... -
Cellular uptake of magnetic nanoparticle is mediated through energy-dependent endocytosis in A549 cells
Jun-Sung Kim, Tae-Jong Yoon, Kyeong-Nam Yu, Mi Suk Noh, Minah Woo, Byung-Geol Kim, Kee-Ho Lee, Byung-Hyuk Sohn, Seung-Bum Park, 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 3 321-326 (6 pages)
Biocompatible silica-overcoated magnetic nanoparticles containing an organic fluorescence dye, rhodamine B isothiocyanate (RITC), within a silica shell [50 nm size, MNP@SiO2(RITC)s] were synthesized. For future application of the MNP@SiO2(RITC)s into diverse areas of research such as drug or gene delivery, bioimaging, and biosensors, detailed information of the cellular uptake process of the nanoparticles is essential. Thus, this study was performed to elucidate the precise mechanism by which... -
Altered expression of thioredoxin reductase-1 in dysplastic bile ducts and cholangiocarcinoma in a hamster model
Byung-IL Yoon, Dae-Yong Kim, Ja-June Jang, Jeong-Hee Han 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 3호 1 211-216 (6 pages)
Thioredoxin reductase 1 (TrxR) is a homodimeric selenoenzyme catalyzing thioredoxin (Trx) in an NADPHdependent manner. With regard to carcinogenesis, these redox proteins have been implicated in cell proliferation, transformation and anti-apoptosis. In the present study, using a hamster cholangiocarcinoma (ChC) model, we evaluated the immunohistochemical expression pattern of TrxR in precancerous lesions and ChCs as well as in normal bile ducts. The goal of this study was to determine the...


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