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2471.

A Novel Protein Elicitor PeBL2, from Brevibacillus laterosporus A60, Induces Systemic Resistance against Botrytis cinerea in Tobacco Plant

Ghulam Hussain Jatoi, Guo Lihua, Yang Xiufen, Muswar Ali Gadhi, Azhar Uddin Keerio, Yusuf Ali Abdulle, Dewen Qiu 한국식물병리학회 The Plant Pathology Journal 11 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 3호 3 208-218 (11 pages)

Ghulam Hussain Jatoi, Guo Lihua, Yang Xiufen, Muswar Ali Gadhi, Azhar Uddin Keerio, Yusuf Ali Abdulle, Dewen Qiu 저
...
- 4,000원
Here, we reported a novel secreted protein elicitor PeBL2 from Brevibacillus laterosporus A60, which can induce hypersensitive response in tobacco (Nicotiana benthamiana). The ion-exchange chromatography, high-performance liquid chromatography (HPLC) and mass spectrometry were performed for identification of protein elicitor. The 471 bp PeBL2 gene produces a 17.22 kDa protein with 156 amino acids containing an 84-residue signal peptide. Consistent with endogenous protein, the recombinant protein...
2472.

Development and Validation of a Perfect KASP Marker for Fusarium Head Blight Resistance Gene Fhb1 in Wheat

Lovepreet Singh, James A Anderson, Jianli Chen, Bikram S Gill, Vijay K Tiwari, Nidhi Rawat 한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 3호 2 200-207 (8 pages)

Lovepreet Singh, James A Anderson, Jianli Chen, Bikram S Gill, Vijay K Tiwari, Nidhi Rawat 저
...
- 3,000원
Fusarium head blight (FHB) is a devastating wheat disease with a significant economic impact. Fhb1 is the most important large effect and stable QTL for FHB resistance. A pore-forming toxin-like (PFT) gene was recently identified as an underlying gene for Fhb1 resistance. In this study, we developed and validated a PFTbased Kompetitive allele specific PCR (KASP) marker for Fhb1. The KASP marker, PFT_KASP, was used to screen 298 diverse wheat breeding lines and cultivars. The KASP clustering...
2473.

A Duplex PCR Assay for Rapid Detection of Phytophthora nicotianae and Thielaviopsis basicola

Na Liu, Shijun Jiang, Songli Feng, Wenyan Shang, Guozhen Xing, Rui Qiu, Chengjun Li, Shujun Li, Wenming Zheng 한국식물병리학회 The Plant Pathology Journal 6 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 2호 9 172-177 (6 pages)

Na Liu, Shijun Jiang, Songli Feng, Wenyan Shang, Guozhen Xing, Rui Qiu, Chengjun Li, Shujun Li, Wenming Zheng 저
...
- 3,000원
A duplex PCR method was developed for simultaneous detection and identification of tobacco root rot pathogens Phytophthora nicotianae and Thielaviopsis basicola. The specific primers for P. nicotianae were developed based on its internal transcribed spacer (ITS) regions of ribosomal gene, ras gene and hgd gene, while the specific primers for T. basicola were designed based on its ITS regions and β-tubulin gene. The specificity of the primers was determined using isolates of P. nicotianae, T....
2474.

DevelDevelopment of a Quantitative Real-time Nucleic Acid Sequence based Amplification (NASBA) Assay for Early Detection of Apple scar skin viroid-time Nucleic Acid Sequence based Amplification (NASBA) Assay for Early Detection of Apple scar skin viroid

Seong Heo, Hyun Ran Kim, Hee Jae Lee 한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 2호 8 164-171 (8 pages)

Seong Heo, Hyun Ran Kim, Hee Jae Lee 저
...
- 3,000원
An assay for detecting Apple scar skin viroid (ASSVd) was developed based on nucleic acid sequence based amplification (NASBA) in combination with realtime detection during the amplification process using molecular beacon. The ASSVd specific primers for amplification of the viroid RNA and molecular beacon for detecting the viroid were designed based on highly conserved regions of several ASSVd sequences including Korean isolate. The assay had a detection range of 1 × 104 to 1 × 1012 ASSVd RNA...
2475.

Factors Affecting the Vineyard Populational Diversity of Plasmopara viticola

Susana Boso, Pilar Gago, Jos?-Luis Santiago, Mar?a de la Fuente, Mar?a-Carmen Mart?nez 한국식물병리학회 The Plant Pathology Journal 12 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 2호 4 125-136 (12 pages)

Susana Boso, Pilar Gago, Jos?-Luis Santiago, Mar?a de la Fuente, Mar?a-Carmen Mart?nez 저
...
- 4,100원
at the molecular level through the examination of microsatellite markers. The populations of P. viticola represented in primary and secondary infections were investigated in the same way. The concentration of airborne sporangia in the vegetative cycle was also examined, as was the virulence of the different P. viticola populations detected. The epidemiological characteristics of the fungus differed depending on the degree of isolation of the vineyard, the airborne spore concentration, and on...
2476.

Chlorophyll a Fluorescence Parameters of Hulled and Hull-less Barley (Hordeum vulgare L.) DH Lines Inoculated with Fusarium culmorum

Tomasz Warzecha, Edyta Skrzypek, Tadeusz Adamski, Maria Surma, Zygmunt Kaczmarek, Agnieszka Sutkowska 한국식물병리학회 The Plant Pathology Journal 13 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 2호 3 112-124 (13 pages)

Tomasz Warzecha, Edyta Skrzypek, Tadeusz Adamski, Maria Surma, Zygmunt Kaczmarek, Agnieszka Sutkowska 저
...
- 4,200원
Barley worldwide is affected seriously by Fusarium seedling blight (FSB) and Fusarium head blight (FHB) diseases caused by the Fusarium species. The objective of this study was to facilitate the resistance of hulled and hull-less barley at different growth stages to F. culmorum according to direct parameters: disease rating (DR), fresh weight of leaves and roots, kernel weight per spike, kernel number per spike, plump kernels, and indirect parameters - chlorophyll a fluorescence (CF). Plate...
2477.

Ascospore Infection and Colletotrichum Species Causing Glomerella Leaf Spot of Apple in Uruguay

Sandra Alaniz, Vanessa Cuozzo, Valentina Mart?nez, Marciel J. Stadnik, Pedro Mondino 한국식물병리학회 The Plant Pathology Journal 12 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 2호 2 100-111 (12 pages)

Sandra Alaniz, Vanessa Cuozzo, Valentina Mart?nez, Marciel J. Stadnik, Pedro Mondino 저
...
- 4,100원
in fruit lesions. Based on phylogenetic analysis of actin, β-tubulin and glyceraldehyde-3-phosphate dehydrogenase gene regions of 46 isolates, 25 from fruits and 21 from leaves, C. karstii was identified for the first time causing GLS in Uruguay and C. fructicola was found to be the most frequent (89%) and aggressive species. The higher aggressiveness of C. fructicola and its ability on to produce abundant fertile perithecia could help to explain the predominance of this species in the field.
2478.

The MAP Kinase Kinase Gene AbSte7 Regulates Multiple Aspects of Alternaria brassicicola Pathogenesis

Kai Lu, Min Zhang, Ran Yang, Min Zhang, Qinjun Guo, Kwang-Hyun Baek, Houjuan Xu 한국식물병리학회 The Plant Pathology Journal 9 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 2호 1 91-99 (9 pages)

Kai Lu, Min Zhang, Ran Yang, Min Zhang, Qinjun Guo, Kwang-Hyun Baek, Houjuan Xu 저
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- 3,000원
Mitogen-activated protein kinase (MAPK) cascades in fungi are ubiquitously conserved signaling pathways that regulate stress responses, vegetative growth, pathogenicity, and many other developmental processes. Previously, we reported that the AbSte7 gene, which encodes a mitogen-activated protein kinase kinase (MAPKK) in Alternaria brassicicola, plays a central role in pathogenicity against host cabbage plants. In this research, we further characterized the role of AbSte7 in the pathogenicity of...
2479.

Rapid Detection of SdhBP225F and SdhBH272R Mutations in Boscalid Resistant Botrytis cinerea Strains by ARMS-PCR

Xin Liu, Rong Zeng, Shigang Gao, Lihui Xu, Fuming Dai 한국식물병리학회 The Plant Pathology Journal 6 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 1호 8 71-76 (6 pages)

Xin Liu, Rong Zeng, Shigang Gao, Lihui Xu, Fuming Dai 저
...
- 3,000원
SdhBP225Fand SdhBH272R mutations have been found associated with boscalid resistance in Botrytis cinerea from strawberry in Shanghai, China. For rapid detection of two mutations, tetra-primers were designed and optimized to gain the relatively high accuracy and specificity based on the ARMS-PCR technique, by which resistance can be identified with different lengths of products on agarose gels. The tetra-primer ARMS-PCR systems for SdhBP225F and SdhBH272R were validated by 9 SdhB-squenced strains...
2480.

Genetic Variability among Different Populations of Root Knot Nematodes Based on Their Encumbrance Response to Pasteuria Isolates Using PCR-RFLP

Muhammad Kamran, Nazir Javed, Ihsan Ullah, Shahid Nazir, Shakra Jamil, Muhammad Zafar Iqbal, Huma Abbas, Sajid Aleem Khan, Muhammad Ehetisham ul Haq 한국식물병리학회 The Plant Pathology Journal 12 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 1호 6 51-62 (12 pages)

Muhammad Kamran, Nazir Javed, Ihsan Ullah, Shahid Nazir, Shakra Jamil, Muhammad Zafar Iqbal, Huma Abbas, Sajid Aleem Khan, Muhammad Ehetisham ul Haq 저
...
- 4,100원
A great variable response was observed when PP-3 and PP-J encumbered with 116 populations of root knot nematode (RKN) at two different temperatures (25 ± 2°C and 30 ± 2°C) and concentrations (104 and 105 spores/ml). The PCR reaction amplified intergenic region between cytochrome oxidase subunit II gene (COII) and large subunit of rRNA gene (lrRNA) of the mitochondrial genome of different RKN species. The primer C2F3 and 1108 identified M. incognita with the highest frequency (52.6%) followed...

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