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Effect of Phytohormones and Chemical Inhibitors on Pathogenesis-related Genes Identified by Differential Hybridization in Rice Suspension Culture Cells
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 4호 13 386-393 (8 pages)
In order to study disease resistance mechanisms in rice against the rice blast fungus Magnaporthe grisea, we screened fungal elicitor-responsive genes from rice suspension-cultured cells treated with fungal elicitors employing differential hybridization (DH). By DH screening, 31 distinct rice clones were isolated and a majority of them were full-length cDNAs encoding pathogenesisrelated (PR) genes. Sixteen of the 31 genes were upregulated at 4, 8, and 12 h following fungal elicitor treatment. To... -
Simple and Reliable DNA Extraction Method for the Dark Pigmented Fungus, Cercospora sojina
한국식물병리학회 The Plant Pathology Journal 4 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 3호 15 289-292 (4 pages)
This study used a modified cetyltrimethylammonium bromide (CTAB) method to efficiently extract DNA from the plant pathogenic fungus Cercospora sojina. Total DNA yield obtained by this method was approximately 1 mg/g of mycelia (fresh weight), and the mean ratio of A260/A280 and A260/A230 were 2.04 and 2.1, respectively. The results of random amplified polymorphic DNA (RAPD) analysis, digestion with restriction enzymes, and Southern hybridization indicated that polysaccharides were effectively... -
Control Efficacy of Phloretin Isolated from Apple Fruits Against Several Plant Diseases
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 3호 13 280-285 (6 pages)
In the course of a searching natural antifungal compounds from plant sources, we found that the methanol extract (3,000 μg/ml) of Malus domestica fruits had potential of control against rice blast (Magnaporthe grisea) and tomato late blight (Phytophthora infestans). Under bioassay-guided purification, we isolated phloretin, a phenolic compound, with in vivo antifungal activity against M. grisea. By 1-day protective application of phloretin (500 μg/ml), the compound strongly inhibited the... -
Production of Surfactin and Iturin by Bacillus licheniformis N1 Responsible for Plant Disease Control Activity
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 2호 10 170-177 (8 pages)
Bacillus licheniformis N1, previously developed as a biofungicide formulation N1E to control gray mold disease of plants, was investigated to study the bacterial traits that may be involved in its biological control activity. Two N1E based formulations, bacterial cell based formulation PN1E and culture supernatant based formulation SN1E, were evaluated for disease control activity against gray mold disease of tomato and strawberry plants. Neither PN1E nor SN1E was as effective as the original... -
Characteristics of Cucumber mosaic virus Infecting Zucchini in Korea
한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 2호 6 139-148 (10 pages)
A virus causing stunt, yellowing, severe mosaic, malformation symptoms on leaves and uneven development and malformation on fruits of zucchini was prevalent around Goseong, Gyeongsangnam-do, Korea. A survey conducted (2004) in the Goseong area revealed about 20% virus infection rate. The disease causative identified as Cucumber mosaic virus (CMV-Z1) was further characterized. The isolate induces mosaic symptoms on Cucumis sativus, while severe mosaic, stunt and malformation on C. pepo. Thin... -
Difference of Physiochemical Characteristics Between Citrus Bacterial Canker Pathotypes and Identification of Korean Isolates with Repetitive Sequence PCRs
한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2008, 24권 4호 8 423-432 (10 pages)
The difference of carbon source utilization and fatty acid composition between the pathotypes of Xanthomonas strains, which causing citrus bacterial canker was compared, and the physiochemical characteristics were used to analyze relationship of the strains for the first time. The pattern of several carbon sources utilization and fatty acids composition reliably discriminated the pathotypes of Xanthomonas strains. The dendrogram which was constructed by 95 carbon source utilization profiles... -
Purification and Phytotoxicity of Apicidins Produced by the Fusarium semitectum KCTC16676
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2008, 24권 4호 7 417-422 (6 pages)
at 25oC, the culture was extracted with chloroform. Apicidins were purified through a reverse phase C18 silica gel column, resulting in 5 g of apicidin, 200 mg of apicidin A, and 300 mg of apicidin D2 from 4 kg of wheat cultures; this represents a significant yield improvement from a previous method, offers more materials to study the modes of its action, and facilitates the elucidation of the apicidin biosynthesis pathway. Apicidin and apicidin D2 showed phytotoxicity on both seedlings and... -
Causal Fungus of Side Rot on ‘Zesy002’ Kiwifruit in Jeju Island, South Korea
Magda Ledesma, Yong Ho Shin, Sonia Whiteman, Joy Tyson, Gyoung Hee Kim, Jeum Kyu Hong, Birgit Zange, Yong Chull Jeun 한국식물병리학회 식물병연구 8 Pages
한국식물병리학회 식물병연구 2021, 27권 1호 4 24-31 (8 pages)
Since the past few years, dimple rot has become one of the most serious diseases affecting Actinidia chinensis var. chinensis ‘Zesy002’ kiwifruit in Korea, leading to a low quality and subsequent losses of income. In the current study, the causal pathogen of side rot on ‘Zesy002’ kiwifruit was identified to be Botryosphaeria dothi- dea, a widespread pathogen that could penetrate the fruit directly without wounding. Diaporthe sp., another pathogen causing dimple rot, was isolated from... -
고랭지 여름 무에서 시들음병 발생 현황과 병원균의 병원학적 특성 연구
홍성기, 고형래, 최효원, 이영기, 김점순 한국식물병리학회 식물병연구 8 Pages
한국식물병리학회 식물병연구 2020, 26권 4호 8 256-263 (8 pages)
2018년과 2019년에 강원도 여름 재배 무 포장에서 시들음병 발생이 조사되었다. 시들음병은 7월 상순 발생했고, 7월 하순과 8월 뜨거운 여름에 급속히 확산되었으며, 심한 경우, 일부 포장에서는 80%까지 발생하였다. 유묘기에 병든 무는 생육이 불량하고 뿌리 내부조직이 갈변되었다. 생육중기 동안시간이 지나면서 병든 잎은 황화되고 뿌리표면은 흰색에서흑색으로 변색되고, 도관조직은 갈변하였다. 병든 식물체로부터 23개 Fusarium균이 분리되었고, EF-1α와 IGS 염기서열 분석에 의해 Fusarium oxysporum f. sp. raphani로... -
Expression Patterns of Transposable Elements in Magnaporthe oryzae under Diverse Developmental and Environmental Conditions
Hyunjung Chung, Seogchan Kang, Yong-Hwan Lee, Sook-Young Park 한국식물병리학회 식물병연구 6 Pages
한국식물병리학회 식물병연구 2020, 26권 1호 5 38-43 (6 pages)
The genome of the rice blast fungus Magnaporthe oryzae contains several types of transposable elements (TEs), and some TEs cause genetic variation that allows M. oryzae to evade host detection. We studied how five abundant TEs in rice pathogens, Pot3, Pot2, MAGGY, Line-like element (MGL) and Mg-SINE, are expressed under diverse conditions related to growth, development, and stress. Expression of Pot3 and Pot2 was ac- tivated in germinated conidia and mycelia treated with tricyclazole....


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