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241.

Analysis of the seroprevalence of bovine paratuberculosis and the application of modified absorbed ELISA to field sample testing in Korea

Kun Taek Park, Jongsam Ahn, William C. Davis, Hye Cheong Koo, Nam Hoon Kwon, Woo Kyung Jung, Jun Man Kim, Soon Keun Hong, Yong H 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 8 349-354 (6 pages)

Kun Taek Park, Jongsam Ahn, William C. Davis, Hye Cheong Koo, Nam Hoon Kwon, Woo Kyung Jung, Jun Man Kim, Soon Keun Hong, Yong H 저
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- 3,000원
commercial ELISA (C-ELISA). Receiver operating characteristic analysis was used to determine the cutoff point for P-ELISA. Based on C-ELISA results, the area under the curve for P-ELISA was 0.913 (95% CI, 0.883 to 0.943). Using a cutoff point of 0.100, P-ELISA showed a sensitivity of 62.0% and a specificity of 93.7%. The kappa value and the percent agreement between the two ELISAs were 0.322 and 92.5%, respectively. Both ELISAs showed a significant correlation between age and seropositivity (p <...
242.

Random amplified polymorphic DNA (RAPD) analysis of Mycobacterium tuberculosis strains in India

J. P. N. Singh, Rishendra Verma, P. Chaudhuri 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 2호 15 181-187 (7 pages)

J. P. N. Singh, Rishendra Verma, P. Chaudhuri 저
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- 3,000원
of polymorphic DNA (RAPD) analysis for typing Indian strains of M. tuberculosis was investigated. M. tuberculosis H37Rv, M. tuberculosis DT and 42 clinical isolates of M. tuberculosis were subjected to RAPD-PCR using 7 random decamer primers. All 7 primers were found to be differentiated and produced specific RAPD profiles. The polymorphic amplicons served as RAPD markers for M. tuberculosis. The dendrograms, obtained by different primers, showed the discriminatory ability of the primers. RAPD...
243.

Analysis of Salmonella enterica serotype Enteritidis isolated from human and chickens by repetitive sequence-PCR fingerprinting, antibiotic resistance and plasmid profiles

Dong Kyun Suh, Jae Chan Song 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 6 37-41 (5 pages)

Dong Kyun Suh, Jae Chan Song 저
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- 1,500원
resistance patterns. The most common pattern was penicillin resistant (63.6%). Only one isolate from chicken showed a multiple drug resistance patterns to 4 antibiotics.All 22 S.Enteritidis isolates harbored more than two plasmids with eight different plasmid profiles including two to six plasmids with approximate molecular size ranging from 1.9 to 21 kb.A band of 15 kb size was detected in all isolates tested, however, the band sizes smaller than 15 kb were found only in isolates from chicken.
244.

Identification and prevalence of Ehrlichia chaffeensis infection in Haemaphysalis longicornis ticks from Korea by PCR, sequencing and phylogenetic analysis based on 16S rRNA gene

Seung-Ok Lee, Dong-Kyeun Na, Chul-Min Kim, Ying-Hua Li, Yoon-Hee Cho, Jin-Ho Park, John-Hwa Lee, Seong-Kug Eo, Terry A. Klein, J 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 10 151-155 (5 pages)

Seung-Ok Lee, Dong-Kyeun Na, Chul-Min Kim, Ying-Hua Li, Yoon-Hee Cho, Jin-Ho Park, John-Hwa Lee, Seong-Kug Eo, Terry A. Klein, J 저
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- 1,500원
the database showed that EC-PGHL was 100% identical or similar to the Arkansas (AF416764), the Sapulpa (U60476) and the 91HE17 (U23503) strains. The phylogenetic analysis also revealed that the E. chaffeensis EC-PGHL formed a single cluster with the above strains. This is the first study to report molecular detection and phylogenetic analysis of E. chaffeensis from H. longicornis ticks in Korea. The implicit significance of E. chaffeensis infection in H. longicornis ticks in Korea is discussed.
245.

Molecular cloning of the cDNA of canine homeodomaininteracting protein kinase 2

Sook-Yeon Lee, Jin-Young Chung, Il-Seob Shin, Eun-Wha Choi, Cheol-Yong Hwang, Hwa-Young Youn, Hong-Ryul Han 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 8 141-145 (5 pages)

Sook-Yeon Lee, Jin-Young Chung, Il-Seob Shin, Eun-Wha Choi, Cheol-Yong Hwang, Hwa-Young Youn, Hong-Ryul Han 저
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- 1,500원
The research of p53 is being conducted to find the mechanisms of tumorigenesis and to treat various cancers. Homeodomain-interacting protein kinase2 (HIPK2) is an important factor to regulate p53 and to increase the stability of p53. Activation of HIPK2 leads to the selective phosphorylation of p53, resulting in growth arrest and the enhancement of apoptosis. In this study, the canine HIPK2 cDNA fragments were obtained, and their overlapping regions were aligned to give a total sequence of 3489...
246.

Identification and antigenic site analysis of foot-and-mouth disease virus from pigs and cattle in Korea

Jae Ku Oem, Kwang Nyeon Lee, In Soo Cho, Soo Jeong Kye, Jee Yong Park, Jong Hyeon Park, Yong Joo Kim, Yi Seok Joo, Hee Jong Song 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 5 117-124 (8 pages)

Jae Ku Oem, Kwang Nyeon Lee, In Soo Cho, Soo Jeong Kye, Jee Yong Park, Jong Hyeon Park, Yong Joo Kim, Yi Seok Joo, Hee Jong Song 저
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- 3,000원
From May to June 2002, a total of 16 foot-and mouth disease (FMD) outbreaks due to the serotype O virus, Pan Asia strain, were recorded in Korea. The viruses were identified by antigen ELISA, RT-PCR and sequence analysis. The overall nucleotide sequence divergence of the VP1 region among the 4 isolates in 2002 was 0 to 1.4%, but between O/SKR/2002 and O/SKR/2000 isolates was 1.9-4.9%. Phylogenetic analysis with the some known strains from East Asian countries showed that the 4 Korean isolates in...
247.

Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD

Ji-Yeon Kim, So-Hyun Kim, Nam-Hoon Kwon, Won-Ki Bae, Ji-Youn Lim, Hye-Cheong Koo, Jun-Man Kim, Kyoung-Min Noh, Woo-Kyung Jung, K 대한수의학회 Journal of Veterinary Science 13 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 1호 2 7-19 (13 pages)

Ji-Yeon Kim, So-Hyun Kim, Nam-Hoon Kwon, Won-Ki Bae, Ji-Youn Lim, Hye-Cheong Koo, Jun-Man Kim, Kyoung-Min Noh, Woo-Kyung Jung, K 저
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- 4,200원
coli O157:H7 Rapid kit were applied, and multiplex PCR and randomly amplified polymorphic DNA (RAPD) were performed for the molecular determination. There was high molecular relatedness among 11 Korean isolates and 17 U. S. strains at 63% level. Additionally, distinct differentiation between pig and cattle isolates was determined. It implied that RAPD had a capacity to distinguish strains with different sources, however it could not discriminate among isolates according to their differences in...
248.

Molecular fingerprinting of clinical isolates of Mycobacterium bovis and Mycobacterium tuberculosis from India by restriction fragment length polymorphism (RFLP)

Sandeep Kumar Singh, Rishendra Verma, Devendra H. Shah 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 8 331-335 (5 pages)

Sandeep Kumar Singh, Rishendra Verma, Devendra H. Shah 저
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- 1,500원
Forty mycobacterial strains comprising clinical Indian isolates of Mycobacterium tuberculosis (28 field isolates +1H37 Rv) and Mycobacterium bovis (10 field isolates +1 AN5) were subjected to restriction fragment length polymorphism analysis (RFLP) using IS6110 and IS1081 probes. Most of these strains originated from dairy cattle herd and human patients from Indian Veterinary research Institute (IVRI) campus isolated from the period of 1986 to 2000. Our study showed presence of 8 copies of...
249.

Molecular characterization of full-length genome of Japanese encephalitis virus (KV1899) isolated from pigs in Korea

Dong Kun Yang, Byoung Han Kim, Chang Hee Kweon, Jun Hun Kwon, Seong In Lim, Hong Ryul Han 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 3호 3 197-205 (9 pages)

Dong Kun Yang, Byoung Han Kim, Chang Hee Kweon, Jun Hun Kwon, Seong In Lim, Hong Ryul Han 저
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- 3,000원
strain and the highest amino acid homology with K94P05. We performed an extensive E gene based phylogenetic analysis on a selection of 41 JEV isolates available from the GenBank. Compared with Anyang strain, isolated from a pig in 1969, that is current live vaccine strain for swine in Korea, the homology of nucleotide sequence in envelope gene was only 87.1%. The prM gene of the isolate was closely related with those of Ishikawa and K94P05 strains, which were grouped into genotype I of JEV.
250.

Two-dimensional gel electrophoresis and immunoblot analysis of Neospora caninum tachyzoites

Eung-Goo Lee, Jae-Hoon Kim, Yong-Seung Shin, Gee-Wook Shin, Yong-Hwan Kim, Gon-Sup Kim, Dae-Yong Kim, Tae-Sung Jung, Myung-Deuk 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 8 139-145 (7 pages)

Eung-Goo Lee, Jae-Hoon Kim, Yong-Seung Shin, Gee-Wook Shin, Yong-Hwan Kim, Gon-Sup Kim, Dae-Yong Kim, Tae-Sung Jung, Myung-Deuk 저
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- 3,000원
Identification of expressed protein profiles and antigenic determination are some of the most challenging aspects of proteomics. Two-dimensional gel electrophoresis (2-DE) combined with immunoblot analysis were employed to study the N. caninum proteome. Protein sample preparation was carried out by first conducting sonication, followed by adding lysis buffer containing 7M urea plus 2M thiourea to the purified tachyzoites in order to complete disruption. A total of 335 differentially expressed...

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