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Transformation of Rabbit Proximal Tubule Cells by Strontium Phosphate Transfection with a Plasmid Containing SV4O Early Region Genes
Han, Ho-Jae, Taub, Mary L., 대한생리학회 대한생리학회지 8 Pages
대한생리학회 대한생리학회지 1994, 제 28권 제 2호 14 233-240 (8 pages)
in a hormone defined medium. Domes were also observed in some of the cultures. 2. r-glutamyl transpeptidase activity was equivalent to that observed in primary renal proximal tubule cell cultures. 3. Transformed cells with pRSVT form tubules in matrigel following 20 passages. 4. Genomic DNA of transformants was digested with either the restriction enzyme Xba or BamH1. A band of approximately 7.5kb was detected with Xba. Three BamH1 bands were detected at approximately 15 kb, 6.5 kb, and 3 kb. -
Rapid Measurement of NH3 and Weak Acid Permeation Through Liposomes and Renal Proximal Tubule Membranes
Bae, Hae-Rahn, Suh, Duck-Joon, Lee, Sang-Ho 대한생리학회 대한생리학회지 12 Pages
대한생리학회 대한생리학회지 1994, 제 28권 제 2호 11 203-214 (12 pages)
Using the methods of stopped-flow and epifluorescence microscopy with entrapped fluorophore, membrane permeability of NH3 and weak acids in liposomes, renal brush border (BBMV) and basolateral membrane vesicles (BLMV), and primary culture cells from renal proximal tubule was measured. Permeability coefficient (cm/sec) of NH3 was (2.9 X 10-2 in phosphatidylcholine liposome 25℃), 5.9 X 10-2 in renal proximal tubule cell (37℃), 4.0 X 10-2 and 2.4 X 10-2 in BBMV and BLMV (25℃), respectively.... -
Calcium-activated Ionic Currents in Smooth Muscle Cells from Rabbit Superior Mesenteric Artery
Lee, Moo-Yeol, Bang, Hyo-Weon, Uhm, Dae-Yong, Rhee, Sang-Don 대한생리학회 대한생리학회지 8 Pages
대한생리학회 대한생리학회지 1994, 제 28권 제 2호 5 151-158 (8 pages)
Intracellular free Ca2+ contributes to regulation of various events occurring in vascular smooth muscle cells. One of these events is modulating the membrane iou currents. Single smooth muscle cells were isolated from rabbit mesenteric artery. Three kinds of Ca2+-activated current were studied with the patch clamp method. Ca2+-activated K+ current with a large oscillation was recorded in the depolarized potential range. The single channel conductance of this current was about 250 pS. It was... -
The Excitatory Mechanism of Substance P in the Antral Circular Muscle of Guinea Pig Stomach
Jun, Jae-Yeoul, Kim, Sung-Joon, Choi, Youn-Baik, Kim, Ki-Whan 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1994, 제 28권 제 1호 5 51-60 (10 pages)
This study was carried out to elucidate the excitatory mechanisms of Substance P in the antral circular muscle, using isometric contraction recording, conventional microelectrode method and whole-cell patch clamp technique. Substance P produced tonic and phasic contractions in a dose-dependent manner and depolarized membrane potential with increased amplitude of slow waves in muscle strips. Voltage-dependent Ca2+ currents were increased by the application of Substance P from a holding potential... -
Effect of Dopamine on the Ca2+-dependent K+ currents in Isolated Single Gastric Myocytes of the Guinea-pig
Rhee, Poong-Lyul, Lee, Sang-Jin, Kim, Sung-Joon, So, In-Suk, Hwang, Sang-Ik, Kim, Ki-Whan 대한생리학회 대한생리학회지 12 Pages
대한생리학회 대한생리학회지 1993, 제 27권 제 2호 3 139-150 (12 pages)
We have reported that dopamine potentiates spontaneous contractions dose-dependently in guinea-pig antral circular muscle strips (Hwang et al, 1991). To clarify the underlying excitatory mechanism of dopamine on the gastric smooth muscle, the effects of dopamine on voltage-dependent Ca2+ currents and Ca2+ -dependent K+ currents were observed in enzymatically dispersed guinea-pig gastric myocytes using the whole-cell voltage-clamp technique. Experiments were also done using isometric tension... -
Effects of Noradrenaline on the Spontaneous Contraction and Ionic Current in the Antral Circular Muscle of Guinea-pig Stomach
Jun, Jae-Yeoul, Lee, Sang-Jin, Kim, Sung-Joon, Suh, Jae-Yul, So, In-Suk, Hwang, Sang-Ik, Kim, Ki-Whan 대한생리학회 대한생리학회지 8 Pages
대한생리학회 대한생리학회지 1993, 제 27권 제 2호 1 115-122 (8 pages)
There is evidence that noradrenaline enhances spontaneous contractions dose-dependently in guinea-pig antral circular muscle. To investigate the mechanism of this excitatory action, slow waves and membrane currents were recorded using conventional microelectrode techniques in muscle strips and the whole cell patch clamp technique in isolated gastric myocytes. On recording slow waves, noradrenaline (10-5 M) induced the hyperpolarization of the membrane potential, although the shape of the slow... -
Cholinergic Control of Pancreatic Secretion: The Effects of Atropine on Plasma Cholecystokinin and Secretin Release
Jo, Yang-Hyeok, Rhie, Duck-Joo, Chang, Young-Soon, Hahn, Sang-June, Sim, Sang-Soo, Kim, Myung-Suk, Kim, Chung-Chin 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1991, 제 25권 제 1호 3 27-36 (10 pages)
Generally, it has been known that cholecystokinin (CCK) release into the plasma is under cholinergic control, but secretin release is not. Thus in anesthetized dogs we studied the effect of atropine (50 μg/kg followed by 50 μg/kg/hr) on pancreatic secretion and plasma concentrations of bioactive CCK and immunoreactive secretin in response to intraduodenal perfusion of sodium oleate (1, 3 and 9 mmol/hr). The volume, protein output and bicarbonate output of the secretion were increased by sodium... -
토끼 신피질 절편에서 PCMB가 유기이온의 이동에 미치는 영향
박인철(Park, In-Cheol), 김태인(Kim, Tae-In), 정동근(Jung, Dong-Keun), 김용근(Kim, Young-Keun) 대한생리학회 대한생리학회지 8 Pages
대한생리학회 대한생리학회지 1990, 제 24권 제 2호 9 345-352 (8 pages)
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가토 신장기능에 미치는 Pro-Atrial Natriuretic Peptide의 영향
이정은(Lee, Jeong-Eun), 조경우(Cho, Kyung-Woo), 김선희(Kim, Suhn-Hee) 대한생리학회 대한생리학회지 14 Pages
대한생리학회 대한생리학회지 1990, 제 24권 제 1호 12 131-144 (14 pages)
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장기간 고염 섭취한 SHR 고혈압 쥐에서, 급성 혈장량 증가에 대한 Atrial Natriuretic Peptide, Aldosterone 및 Renin 분비 반응의 비교
김애라(Kim, Ae-Ra), 이원정(Lee, Won-Jung), 주영은(Choo, Young-Eun), 김선희(Kim, Suhn-Hee), 조경우(Cho, Kyung-Woo) 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1989, 제 23권 제 2호 3 253-262 (10 pages)
장기적으로 소금량을 다르게 섭취시킴에 따라서, 체내의 Na 대사에 관여하는 호르몬인 aldosterone, atrial natriuretic peptide (ANP) 및 renin 분비와 신장의 배설 반응에 나타나는 변화를 정상 혈압쥐 Wistar와 spontaneously hypertensive rat (SHR)에서 비교하고자 실험하였다. 생후 7주의 숫쥐인 Wistar와 SHR에게 저염과 고염 식이 (각각 2, 25 mmol Na/100 g diet)를 6주간 먹였다. 그 후 ether 마취하에서 대퇴 동맥과 정맥 및 방광에 관을 삽입한 후, restraining cage에 넣었다. 수술회복 후 안정시 뇨와 혈액을 채취한 후,...


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