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All blood, No stool: enterohemorrhagic Escherichia coli O157:H7 infection
Jang W. Yoon, Carolyn J. Hovde 대한수의학회 Journal of Veterinary Science 14 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 3호 1 219-232 (14 pages)
Enterohemorrhagic Escherichia coli serotype O157:H7 is a pathotype of diarrheagenic E. coli that produces one or more Shiga toxins, forms a characteristic histopathology described as attaching and effacing lesions, and possesses the large virulence plasmid pO157. The bacterium is recognized worldwide, especially in developed countries, as an emerging food-borne bacterial pathogen, which causes disease in humans and in some animals. Healthy cattle are the principal and natural reservoir of E.... -
Efficacy of nano-hydroxyapatite prepared by an aqueous solution combustion technique in healing bone defects of goat
Samit Kumar Nandi, Biswanath Kundu*, Samir Kumar Ghosh, Dipak Kumar De, Debabrata Basu 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 2호 9 183-192 (10 pages)
The present study was undertaken to evaluate porous hydroxyapatite (HAp), the powder of which was prepared by a novel aqueous solution combustion technique, as a bone substitute in healing bone defects in vivo, as assessed by radiologic and histopathologic methods, oxytetracycline labeling, and angiogenic features in Bengal goat. Bone defects were created in the diaphysis of the radius and either not filled (group I) or filled with a HAp strut (group II). The radiologic study in group II showed... -
In vivo morphological and antigenic characteristics of Photobacterium damselae subsp. piscicida
Tae S. Jung*, Kim D. Thompson, Donatella Volpatti, Marco Galeotti, A. Adams 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 2호 7 169-176 (8 pages)
The present study was conducted to examine the morphology and antigenicity of Photobacterium damselae subsp. piscicida by culturing the bacterium in vivo in the peritoneal cavity of sea bass (Dicentrarchus labrax) within dialysis bags with either a low molecular weight (LMW) cut-off of 25 kDa or a high molecular weight (HMW) cut-off of 300 kDa. Differences were observed in the growth rate between the bacteria cultured in vivo or in vitro. Bacteria cultured in vivo were smaller and produced a... -
The C-terminal variable domain of LigB from Leptospira mediates binding to fibronectin
Yi-Pin Lin, Yung-Fu Chang* 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 2호 3 133-144 (12 pages)
Adhesion through microbial surface components that recognize adhesive matrix molecules is an essential step in infection for most pathogenic bacteria. In this study, we report that LigB interacts with fibronectin (Fn) through its variable region. A possible role for LigB in bacterial attachment to host cells during the course of infection is supported by the following observations: (i) binding of the variable region of LigB to Madin-Darby canine kidney (MDCK) cells in a dose-dependent manner... -
An inactivated vaccine to control the current H9N2 low pathogenic avian influenza in Korea
Jun Gu Choi, Youn Jeong Lee, *, Yong Joo Kim, Eun Kyoung Lee, Ok Mi Jeong, Haan Woo Sung, Jae Hong Kim, Jun Hun Kwon1 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 1호 9 67-74 (8 pages)
amino acid motif (PATSGR/GLF) and had only three amino acid changes (T133N, V216G, E439D, H3 numbering) in the hemagglutinin molecule, as well as 18 amino acid deletions (55-72) and one amino acid change (E54D) in the NA stalk region. The amino acid changes did not significantly affect the antigenicity of the vaccine virus when tested by hemagglutination inhibition assay. Though not complete, the vaccine produced after the 20th passage of the virus (01310 CE20) showed good protection against a... -
Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules
William C. Davis*, Mary Jo Hamilton 대한수의학회 Journal of Veterinary Science 16 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 1호 8 51-66 (16 pages)
Flow cytometry was used to identify and characterize monoclonal antibodies (mAbs) that react with rabbit leukocyte differentiation molecules (LDM). Screening sets of mAbs, developed against LDM in other species, for reactivity with rabbit LDM yielded 11 mAbs that recognize conserved epitopes on rabbit LDM orthologues and multiple mAbs that recognize epitopes expressed on the major histocompatibility class I or class II molecules. Screening of mAbs submitted to the Animal Homologues Section of... -
Serum immunoglobulin fused interferon-Ձ inhibited tumor growth in athymic mice bearing colon 26 adenocarcinoma cells
un-Sung Kim, Kyeong Nam Yu, Mi Suk Noh, , Min-Ah Woo, , Sung-Jin Park, Jin Hong Park, Jin Hua, Hyun Sun Cho, Soon Kyung Hwang, Eun 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 1호 7 45-50 (6 pages)
Interferon (IFN) has therapeutic potential for a wide range of infectious and proliferative disorders. However, the half-life of IFN is too short to have a stable therapeutic effect. To overcome this problem, serum immunoglobulin has been fused to IFN. In this study, the efficacy of serum immunoglobulin fused INFs (si-IFN1 and si-IFN2) was evaluated on athymic mice bearing colon 26 adenocarcinoma cells. Seven days after the implantation of tumor cells, each group of mice was injected once a week... -
Increased phosphorylation of caveolin-1 in the spinal cord of irradiated rats
Heechul Kim, Changjong Moon, Jeongtae Kim, Meejung Ahn, Jin Won Hyun, Jae Woo Park, Sung-Ho Kim, Seungjoon Kim, Taekyun Shin* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 4호 2 323-328 (6 pages)
Phosphorylation of caveolin-1 occurs during cell activation by various stimuli. In this study, the involvement of caveolin-1 in an irradiation injured spinal cord was examined by analyzing the phosphorylation of caveolin-1 in the spinal cord of rats after irradiation with a single dose of 15 Gray from a Co ■-ray source at 24 h post-irradiation (PI). A Western blot analysis showed that the phosphorylated form of caveolin-1 (p-caveolin-1) was expressed constitutively in the normal spinal cords... -
Variation in the molecular weight of Photobacterium damselae subsp. piscicida antigens when cultured under different conditions in vitro
Tae S. Jung, Kim D. Thompson, Donatella Volpatti, Marco Galeotti, A. Adams 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 3호 9 255-261 (7 pages)
The antigenicity of Photobacterium damselae (Ph. d.) subsp. piscicida, cultured in four different growth media [tryptone soya broth (TSB), glucose-rich medium (GRM), iron-depleted TSB (TSB + IR-), and iron-depleted GRM (GRM + IR-)] was compared by enzyme-linked immunosorbent assay (ELISA) and Western blot analysis using sera obtained from sea bass (Dicentrarchus labrax) raised against live or heat-killed Ph. d. subsp. piscicida. The antigenic expression of Ph. d. subsp. piscicida was found to... -
Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus
Amr E. El Hakim, Yasser E. Shahein, Amira M. K. Abouelella, Mohamed E. Selim 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 12 175-180 (6 pages)
The present study was conducted to identify new target immunogenic molecules from the larval stage of the cattle tick, Boophilus annulatus (Acari: Ixodidae). Two specific larval glycoproteins (GLPs) were isolated by two-step affinity chromatography. The larval immunogens were first purified with CNBr-Sepharose coupled to rabbit anti-larval immunoglobulins, and the glycoproteins were then purified with Con-A Sepharose. These glycoproteins have molecular weights of approximately 32 and 15 kDa with...


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