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251.

Conditional knockout of brca1/2 and p53 in mouse ovarian surface epithelium: Do they play a role in ovarian carcinogenesis?

Ki-Yon Kim, Dong Wook Park, Eui-Bae Jeung, Kyung-Chul Choi* 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 4호 3 291-297 (7 pages)

Ki-Yon Kim, Dong Wook Park, Eui-Bae Jeung, Kyung-Chul Choi* 저
...
- 3,000원
Alterations of genes are known to be critical for the induction of tumorigenesis, but the mechanism of ovarian carcinogenesis is little understood and remains to be elucidated. In this study, we investigated the roles of brca1, brca2 and p53 genes in the development of ovarian cancer using conditional knockout mice generated by a Cre-loxP recombinant system. Following the application of recombinant adenovirus expressing Cre in vitro, the proliferation of ovarian surface epithelium (OSE) was...
252.

Simultaneous detection and subtyping of porcine endogenous retroviruses proviral DNA using the dual priming oligonucleotide system

Hyoung-Joon Moon, Seong-Jun Park, Hye-Kwon Kim, Soo-Kyung Ann, Semi Rho, Hyun-Ok Keum, Bong-Kyun Park* 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 14 269-271 (3 pages)

Hyoung-Joon Moon, Seong-Jun Park, Hye-Kwon Kim, Soo-Kyung Ann, Semi Rho, Hyun-Ok Keum, Bong-Kyun Park* 저
...
- 1,500원
The purpose of this study was to develop a multiplex PCR that can detect porcine endogenous retrovirus (PERV) proviral genes (pol, envA, envB, envC) and porcine mitochondrial DNA, using a dual priming oligonucleotide (DPO) system. The primer specifically detected the PERV proviral genes pol, envA, envB, envC, and porcine mitochondrial DNA only in samples of pig origin. The sensitivity of the primer was demonstrated by simultaneous amplification of all 5 target genes in as little as 10 pg of pig...
253.

Development and evaluation of a multiplex PCR assay for simultaneous detection of Flavobacterium psychrophilum, Yersinia ruckeri and Aeromonas salmonicida subsp. salmonicida in culture fisheries

Ertan Emek Onuk, Alper Ciftci*, Arzu Findik, Yuksel Durmaz 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 9 235-241 (7 pages)

Ertan Emek Onuk, Alper Ciftci*, Arzu Findik, Yuksel Durmaz 저
...
- 3,000원
Bacterial cold water disease, enteric red mouth disease and frunculosis are the common bacterial diseases of fish worldwide. The etiologic agents of these diseases are Flavobacterium (F.) psychrophilum, Yersinia (Y.) ruckeri and Aeromonas (A.) salmonicida subsp. salmonicida, respectively. In this study, a multiplex polymerase chain reaction (m-PCR) method with YER8/10-Fer3/4-FP1/3 primer pairs which can identify these fish pathogens simultaneously was developed and optimized. In optimized...
254.

Molecular heterogeneity of plpE gene in Indian isolates of Pasteurella multocida and expression of recombinant PlpE in vaccine strain of P. multocida serotype B: 2

Ajay Pratap Singh, Satparkash Singh, Rajeev Ranjan*, Santosh Kumar Gupta, Vijendra Pal Singh, Bhaskar Sharma 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 8 227-233 (7 pages)

Ajay Pratap Singh, Satparkash Singh, Rajeev Ranjan*, Santosh Kumar Gupta, Vijendra Pal Singh, Bhaskar Sharma 저
...
- 3,000원
1 was amplified from the genomic DNA. The amplified products were cloned and the nucleotide sequence was determined. Sequence analysis of the recombinant clones revealed a single open reading frame of 1,011 bp, 1,008 bp and 1,017 bp encoding a protein with a calculated molecular mass of 37.829 kDa, 37.389 kDa and 37.965 kDa for serotypes A: 3, B: 2 and D: 1 respectively. The comparison of the plpE sequence in different capsular types revealed a high degree (＞90%) of homology. Furthermore, the...
255.

Antigenic and immunogenic investigation of the virulence motif of the Newcastle disease virus fusion protein

Kang-Seuk Choi*, Eun-Kyoung Lee, Woo-Jin Jeon, Jun-Hun Kwon 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 5 205-211 (7 pages)

Kang-Seuk Choi*, Eun-Kyoung Lee, Woo-Jin Jeon, Jun-Hun Kwon 저
...
- 3,000원
have a multibasic amino acid sequence (virulence motif) such as RRQKRF at the cleavage site of the precusor fusion (F0) protein. The antigenic and immunogenic characteristics of the virulence motif RRQKRF in the F0 protein of virulent NDVs were investigated. Epitope mapping analysis revealed that a RRQKRF-specific monoclonal antibody 4G2 recognized the KRF section of the motif. A synthetic peptide bearing the RRQKRF motif reacted strongly with sera from virulent NDV (with RRQKRF motif)-infected...
256.

Evaluation of PCR inhibitory effect of enrichment broths and comparison of DNA extraction methods for detection of Salmonella Enteritidis using real-time PCR assay

Ji-Yeon Hyeon, In-Gyun Hwang, Hyo-Sun Kwak, Chankyu Park, In-Soo Choi, Kun-Ho Seo 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 8 143-149 (7 pages)

Ji-Yeon Hyeon, In-Gyun Hwang, Hyo-Sun Kwak, Chankyu Park, In-Soo Choi, Kun-Ho Seo 저
...
- 3,000원
with an additional washing step or the DNeasy Tissue Kit. In three experiments, when applied to detection of Salmonella Enteritidis in steamed pork, the real-time PCR coupled with single 24 h enrichment with BPW performed better than double 48 h enrichment with BPW plus RV or MKTTn. The simple real-time PCR assay using BPW proved to be a rapid and sensitive test for detection of low concentrations of Salmonella Enteritidis in steamed pork samples as compared with the conventional culture method.
257.

Evaluation of infectivity and transmission of different Asian foot-and-mouth disease viruses in swine

Juan M. Pacheco, P. W. Mason 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 7 133-142 (10 pages)

Juan M. Pacheco, P. W. Mason 저
...
- 3,000원
Most isolates of foot-and-mouth disease virus (FMDV) display a broad host range. Since the late 1990s, the genetic lineage of PanAsia topotype FMDV serotype O has caused epidemics in the Far East, Africa, the United Kingdom, France, the Netherlands, and numerous other countries throughout Europe and Asia. In contrast, there are several FMDV isolates that exhibit a more restricted host range. A Cathay topotype isolate of FMDV serotype O from the 1997 epizootic in Taiwan (O/TAW/97) demonstrated...
258.

spa typing and enterotoxin gene profile of Staphylococcus aureus isolated from bovine raw milk in Korea

Sun Young Hwang, Young Kyung Park, Hye Cheong Koo, Yong Ho Park 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 6 125-131 (7 pages)

Sun Young Hwang, Young Kyung Park, Hye Cheong Koo, Yong Ho Park 저
...
- 3,000원
Staphylococcus aureus is a major etiological pathogen of bovine mastitis, which triggers significant economic losses in dairy herds worldwide. In this study, S. aureus strains isolated from the milk of cows suffering from mastitis in Korea were investigated by spa typing and staphylococcal enterotoxin (SE) gene profiling. Forty-four S. aureus strains were isolated from 26 farms in five provinces. All isolates grouped into five clusters and two singletons based on 14 spa types. Cluster 1 and 2...
259.

Detection of Streptococcus dysgalactiae subsp. equisimilis in equine nasopharyngeal swabs by PCR

Silvia Preziuso, *, Fulvio Laus, Aurora Romero Tejeda, Carlo Valente, Vincenzo Cuteri2 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 1호 10 67-72 (6 pages)

Silvia Preziuso, *, Fulvio Laus, Aurora Romero Tejeda, Carlo Valente, Vincenzo Cuteri2 저
...
- 3,000원
a history of S. equisimilis infection and were tested blindly by bacteriological isolation and by single and duplex PCR. DNA for PCR was extracted both from the colonies grown on agar media and from enrichment broth aliquots after incubation with nasal swab samples. S. equisimilis was identified by bacteriological isolation in 23 out of 99 swab samples, and PCR assays on these colonies were fully concordant with bacteriological identification (kappa statistic = 1.00). In addition, PCR of the...
260.

Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation

Sandra Branco, Jo?o Orvalho, Alexandre Leit?o, Isadora Pereira, Manuel Malta, Isabel Mariano, T?nia Carvalho, Rui Baptista, Bria 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 1호 5 27-34 (8 pages)

Sandra Branco, Jo?o Orvalho, Alexandre Leit?o, Isadora Pereira, Manuel Malta, Isabel Mariano, T?nia Carvalho, Rui Baptista, Bria 저
...
- 3,000원
This study was carried out to investigate fifteen cases of acute lethal infection of calves (≤ 4 months of age) by the protozoan parasite Theileria (T.) annulata in the south of Portugal. Calves developed multifocal to coalescent nodular skin lesions, similar to multicentric malignant lymphoma. Infestation with ticks (genus Hyalomma) was intense. Theileria was seen in blood and lymph node smears, and T. annulata infection was confirmed by isolation of schizont-transformed cells and sequencing...

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