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Effect of Probenecid on Tetraethylammonium (TEA) Transport Across Basolateral Membrane of Rabbit Proximal Tubule
Choi, Tae-Ryong, Kim, Yong-Keun, 대한생리학회 대한생리학회지 8 Pages
대한생리학회 대한생리학회지 1996, 제 30권 제 2호 7 249-256 (8 pages)
of tetraethylammonium (TEA) was studied in renal cortical slices and isolated membrane vesicles to investigate the interaction of organic anion with the organic cation transport system in proximal tubule. Probenecid reversibly inhibited TEA uptake by renal cortical slices in a dose-dependent manner over the concentration range of 1 and 5 mM. The efflux of TEA was not affected by the presence of 3 mM probenecid. Kinetic analysis indicated that probenecid decreased Vmax without significant change... -
Decreased GLUT 4 mRNA Levels did not Related with Degree of Hyperglycemia in Skeletal Muscles of Streptozotocin-induced Diabetic Rats
Park, So-Young, Kim, Jong-Yeon, Kim, Yong-Woon, Lee, Suck-Kang 대한생리학회 대한생리학회지 6 Pages
대한생리학회 대한생리학회지 1996, 제 30권 제 2호 5 231-236 (6 pages)
In our previous study (Kim et al, 1991), GLUT 4 protein content correlated negatively with plasma glucose levels in skeletal muscles of STZ-induced diabetic rats. Thus, in this study, to confirm whether expression of GLUT 4 correlate negatively with degree of hyperglycemia, we measured levels of GLUT 4 mRNA in red and white gastrocnemius muscles in STZ-induced mild and severe diabetic rats. Rats were randomly assigned to control, mild, and severe diabetic groups, and the diabetes was induced by... -
Transepithelial Transport of Organic Cation and Its Inhibition by Sulfhydryl and Carboxyl Reagents in Opossum Kidney Cell Monolayer
Woo, Jae-Suk, Oh, Se-Ok, Jung, Jin-Sup, Kim, Yong-Keun, Lee, Sang-Ho 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1996, 제 30권 제 1호 6 53-62 (10 pages)
Transepithelial transport of tetraethylammonium (TEA) was studied in monolayers of opossum kidney cells cultured on permeable membrane filters. [14C]-TEA was transported across the OK cell monolayer from basolateral to apical side by a saturable process which can be stimulated by acidification of the apical medium. The apparent Michaelis-Menten constant (Km) and the maximum velocity(Vmax) for the transport were 41 μM and 147 pmole/ mg protein/ min, respectively. The transport was significantly... -
Identification of Adenosine 5 -Tetraphosphate in Rabbit Platelets and its Metabolism in Blood
Lee, Joong-Woo, Jeon, Sang-Jun, Kong, In-Deok, Jeong, Seong-Woo 대한생리학회 대한생리학회지 8 Pages
대한생리학회 대한생리학회지 1995, 제 29권 제 2호 3 217-224 (8 pages)
Adenosine 5 -tetraphosphate (ATPP) was identified and quantified in extracts of rabbit platelets by elution of extracts containing authentic adenosine 5 -tetraphosphate and comparison of retention time with nucleotide standards using high-performance liquid chromatography technique. The amount of adenosine 5 -tetraphosphate was 0.62 nmoles/109 cells which was 62-fold lower than that of ATP but only 10-fold lower than that of ADP. During platelet aggregation induced by thrombin, adenosine 5... -
Effects of Insulin and IGFS on Growth and Functional Differentiation in Primary Cultured Rabbit Kidney Proximal Tubule Cells -Growth and membrane transport-
Han, Ho-Jae, Park, Kwon-Moo, 대한생리학회 대한생리학회지 12 Pages
대한생리학회 대한생리학회지 1995, 제 29권 제 2호 1 191-202 (12 pages)
{γ}-glutamyl transpeptidase) were observed to be diminished after the cells were placed in the culture environment. 4. The uptake of α-MG, Pi and Na was significantly increased in cells incubated with insulin or IGF-I, IGF-II had no effect on the uptake of these substrates. 5. Na-pump activity, as assayed by Rb uptake, was significantly increased in cells treated with insulin or IGFs. In conclusion, insulin and IGF-I exert stimulatory effects on growth and membrane transporter(glucose, Na, Pi... -
Effects of High Glucose on Na,K-ATPase and Na/glucose Cotransporter Activity in Primary Rabbit Kidney Proximal Tubule Cells
Han, Ho-Jae 대한생리학회 대한생리학회지 12 Pages
대한생리학회 대한생리학회지 1995, 제 29권 제 1호 7 69-80 (12 pages)
a greater extent when Na+ was omitted from the uptake buffer (81.86% or 86.73% inhibition was observed, respectively). In cell homogenates derived from the primary cells grown in 5 mM glucose medium, the specific activity of the Na/K-ATPase (6.17±1.27 μmole Pi/mg protein/hr) was 1.56 fold lower than the values in cell homogenates treated with 360 mg/dl D-glucose, 20 mM (9.67±1.22 μmole Pi/mg protein/hr). Total Rb+ uptake occurred at a significantly higher rate (1.60 fold increase) in primary... -
Transformation of Rabbit Proximal Tubule Cells by Strontium Phosphate Transfection with a Plasmid Containing SV4O Early Region Genes
Han, Ho-Jae, Taub, Mary L., 대한생리학회 대한생리학회지 8 Pages
대한생리학회 대한생리학회지 1994, 제 28권 제 2호 14 233-240 (8 pages)
In this study, it was investigated whether immortalized proximal tubule cells transformed with pRSVT could survive through the numerous passages. Results were as follows: 1. The cells transfected with pRSVT formed rapidly growing, multilayered colonies within 2 weeks in a hormone defined medium. Domes were also observed in some of the cultures. 2. r-glutamyl transpeptidase activity was equivalent to that observed in primary renal proximal tubule cell cultures. 3. Transformed cells with pRSVT... -
The Change of Secretory Activity of the Alveolar Type ll Cell During Acute Alveolar Injury Induced by N-Nitroso-N-Methylurethane
Lee, Young-Man, Bang, In-Sook, Lee, Suck-Kang 대한생리학회 대한생리학회지 8 Pages
대한생리학회 대한생리학회지 1994, 제 28권 제 1호 7 71-78 (8 pages)
In the animal model of acute respiratory distress syndrome (ARDS) induced by N-nitroso-N-methylurethane (NNNMU) the secretory activity of alveolar type H cells during acute alveolar injury was investigated by determining phospholipid and pulmonary surfactant associated proteins in crude surfactant. The mechanism of the secretory change was studied by determination of DNA and RNA levels in the lung tissue. After induction of acute alveolar injury with NNNMU, pulmonary hemorrhage, atelectasis and... -
Effect of Temperature on Dicarboxylate Transport in Plasma Membrane Vesicles of Rabbit Proximal Tubule
Han, Kyung-Moon, Kim, Young-Hee, Woo, Jae-Suk, Kim, Yong-Keun 대한생리학회 대한생리학회지 6 Pages
대한생리학회 대한생리학회지 1993, 제 27권 제 2호 11 227-232 (6 pages)
The temperature dependence of Na+-dependent succinate uptake was studied in brush border (BBMV) and basolateral (BLMV) membrane vesicles isolated from the rabbit kidney cortex. The succinate uptake was markedly altered by temperature in a similar fashion in both membranes. The temperature dependence was characterized by a nonlinear Arrhenius plot with a break point at 22 and 25℃ for BBMV and BLMV, respectively. The activation energy was 3.91 and 17.09 kcal/mole at above and below the break... -
Maximum Oxygen Consumption Determined by the Bruce and Inclined Treadmill Protocols
Kim, Kyu-Hwa, Kim, Hyeong-Jin, Kim, Kyoung-Soon 대한생리학회 대한생리학회지 8 Pages
대한생리학회 대한생리학회지 1993, 제 27권 제 2호 9 209-216 (8 pages)
The aim of the present study was to derive regression equations for V̇o2max vs. V̇o2peak, and V̇O2 vs. heart rate, exercise time, and other variables from maximal exercise tests on a treadmill using the Bruce and inclined protocols. Twelve male and 10 female Korean college students aged between 19 and 23 years voIunteered for this study. After the resting measurements, the subjects performed a maximal exercise on a treadmill according to the Bruce protocol. When the resting...


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