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251.

In Vitro and In Vivo Inhibitory Effects of Gaseous Chlorine Dioxide against Fusarium oxysporum f. sp. batatas Isolated from Stored Sweetpotato: Study II

Ye Ji Lee, Jin-Ju Jeong, Hyunjung Jin, Wook Kim, Young Chull Jeun, Gyeong-Dan Yu, Ki Deok Kim 한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 5호 5 437-444 (8 pages)

Ye Ji Lee, Jin-Ju Jeong, Hyunjung Jin, Wook Kim, Young Chull Jeun, Gyeong-Dan Yu, Ki Deok Kim 저
...
- 3,000원
Chlorine dioxide (ClO2) has been widely used as an effective disinfectant to control fungal contamination during postharvest crop storage. In this study, Fusarium oxysporum f. sp. batatas SP-f6 from the black rot symptom of sweetpotato was isolated and identified using phylogenetic analysis of elongation factor 1-α gene; we further examined the in vitro and in vivo inhibitory activities of ClO2 gas against the fungus. In the in vitro medium tests, fungal population was significantly inhibited...
252.

DevelDevelopment of a Quantitative Real-time Nucleic Acid Sequence based Amplification (NASBA) Assay for Early Detection of Apple scar skin viroid-time Nucleic Acid Sequence based Amplification (NASBA) Assay for Early Detection of Apple scar skin viroid

Seong Heo, Hyun Ran Kim, Hee Jae Lee 한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 2호 8 164-171 (8 pages)

Seong Heo, Hyun Ran Kim, Hee Jae Lee 저
...
- 3,000원
An assay for detecting Apple scar skin viroid (ASSVd) was developed based on nucleic acid sequence based amplification (NASBA) in combination with realtime detection during the amplification process using molecular beacon. The ASSVd specific primers for amplification of the viroid RNA and molecular beacon for detecting the viroid were designed based on highly conserved regions of several ASSVd sequences including Korean isolate. The assay had a detection range of 1 × 104 to 1 × 1012 ASSVd RNA...
253.

Loop-mediated Isothermal Amplification assay for Detection of Candidatus Liberibacter Asiaticus, a Causal Agent of Citrus Huanglongbing

Cheol Woo Choi, Jae Wook Hyun, Rok Yeon Hwang, Charles A Powell 한국식물병리학회 The Plant Pathology Journal 7 Pages

한국식물병리학회 The Plant Pathology Journal 2018, 34권 6호 5 499-505 (7 pages)

Cheol Woo Choi, Jae Wook Hyun, Rok Yeon Hwang, Charles A Powell 저
...
- 3,000원
Huanglongbing (HLB, Citrus greening disease) is one of the most devastating diseases that threaten citrus production worldwide. Although HLB presents systemically, low titer and uneven distribution of these bacteria within infected plants can make reliable detection difficult. It was known loop-mediated isothermal amplification (LAMP) method has the advantages of being highly specific, rapid, efficient, and laborsaving for detection of plant pathogens. We developed a new LAMP method targeting...
254.

Genomics-based Sensitive and Specific Novel Primers for Simultaneous Detection of Burkholderia glumae and Burkholderia gladioli in Rice Seeds

Chae-yeong Lee, Hyun-Hee Lee, Mohamed Mannaa, Nam-gyu Kim, Jung-wook Park, Ju-yun Kim, Young-Su Seo 한국식물병리학회 The Plant Pathology Journal 9 Pages

한국식물병리학회 The Plant Pathology Journal 2018, 34권 6호 4 490-498 (9 pages)

Chae-yeong Lee, Hyun-Hee Lee, Mohamed Mannaa, Nam-gyu Kim, Jung-wook Park, Ju-yun Kim, Young-Su Seo 저
...
- 3,000원
size. Both primer pairs displayed a high degree of sensitivity for detection of B. glumae and B. gladioli separately in monoplex PCR or simultaneously in duplex PCR from both extracted gDNA and directly preheated bacterial cell suspensions. Limit of detection was as low as 0.1 ng of gDNA of both species and 3.86 × 102 cells for B. glumae and 5.85 × 102 cells for B. gladioli. On inoculated rice seeds, the designed primers could separately or simultaneously detect B. glumae and B. gladioli with...
255.

Disinfection by Ozone Microbubbles Can Cause Morphological Change of Fusarium oxysporum f. sp. melonis Spores

Masahiko Tamaki, Fumiyuki Kobayashi, Hiromi Ikeura, Michio Sato 한국식물병리학회 The Plant Pathology Journal 6 Pages

한국식물병리학회 The Plant Pathology Journal 2018, 34권 4호 8 335-340 (6 pages)

Masahiko Tamaki, Fumiyuki Kobayashi, Hiromi Ikeura, Michio Sato 저
...
- 3,000원
To investigate the difference in the disinfectant efficiency of ozone microbubbles (O3MB) and ozone millibubbles (O3MMB), the morphological change of the treated Fusarium oxysporum f. sp. melonis spores was observed with scanning and transmission electron microscopies (SEM and TEM). The disinfectant efficiency of O3MB on F. oxysporum f. sp. melonis spores was greater than that of O3MMB. On observation with SEM, it was revealed that morphological change of F. oxysporum f. sp. melonis spores was...
256.

Biocontrol Efficacy of Formulated Pseudomonas chlororaphis O6 against Plant Diseases and Root-Knot Nematodes

한국식물병리학회 The Plant Pathology Journal 9 Pages

한국식물병리학회 The Plant Pathology Journal 2018, 34권 3호 10 241-249 (9 pages)

저
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- 3,000원
Commercial biocontrol of microbial plant diseases and plant pests, such as nematodes, requires field-effective formulations. The isolate Pseudomonas chlororaphis O6 is a Gram-negative bacterium that controls microbial plant pathogens both directly and indirectly. This bacterium also has nematocidal activity. In this study, we report on the efficacy of a wettable powder-type formulation of P. chlororaphis O6. Culturable bacteria in the formulated product were retained at above 1 × 108...
257.

Development qRT-PCR Protocol to Predict Strawberry Fusarium Wilt Occurrence

한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2018, 34권 3호 2 163-170 (8 pages)

저
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- 3,000원
Strawberry Fusarium wilt disease, caused by Fusarium oxysporum f. sp. fragariae, is the most devastating disease in strawberry production. The pathogen produces chlamydospores which tolerate against harsh environment, fungicide and survive for decades in soil. Development of detection and quantification techniques are regarded significantly in many soilborne pathogens to prevent damage from diseases. In this study, we improved specific-quantitative primers for F. oxysporum f. sp. fragariae to...
258.

Specific and Sensitive Primers Developed by Comparative Genomics to Detect Bacterial Pathogens in Grains

한국식물병리학회 The Plant Pathology Journal 9 Pages

한국식물병리학회 The Plant Pathology Journal 2018, 34권 2호 3 104-112 (9 pages)

저
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- 3,000원
a detection limit of Pss and Cmn was 2 pg/μl of genomic DNA per PCR reaction, while the detection limit for Rt primers was higher. The selected primers could also detect bacterial cells up to 8.8 × 103 cfu to 7.84 × 104 cfu per gram of grain seeds artificially infected with corresponding bacterial pathogens. The primer pairs and PCR assay developed in this study provide an accurate and rapid detection method for three bacterial pathogens of grains, which can be used to...
259.

Management of Citrus Canker in Argentina, a Success Story

한국식물병리학회 The Plant Pathology Journal 9 Pages

한국식물병리학회 The Plant Pathology Journal 2017, 33권 5호 1 441-449 (9 pages)

저
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- 3,000원
that have been used to control citrus canker at this time. We think the process might be usefull to other countries with the same situation. Results from more than 40 years of research in Northeast (NE) Argentina indicate that we are at the limit of favorable environment for the disease. The severity of citrus canker is greatly affected by the environment and El Niño Southern Oscillation (ENSO) phenomenon which causes cyclic fluctuations on the disease intensity in the NE region....
260.

Development of Multiplex PCR for Simultaneous Detection of Citrus Viruses and the Incidence of Citrus Viral Diseases in Late-Maturity Citrus Trees in Jeju Island

한국식물병리학회 The Plant Pathology Journal 11 Pages

한국식물병리학회 The Plant Pathology Journal 2017, 33권 3호 9 307-317 (11 pages)

저
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- 4,000원
Satsuma dwarf virus (SDV) or Citrus mosaic sadwavirus (CiMV) were not consistently detected in RT-PCR assay with the primer sets based on gene of Japan isolates. SDV and CiMV isolates were distinctively divided into two groups based on phylogenetic analysis of PP2 gene cloned from 22 Korean isolates, and the Korean CiMV and SDV isolates shared 95.5–96.2% and 97.1–97.7% sequence identity with Japanese isolate, respectively. We developed PP2-1 primer set based on the PP2 gene...

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