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Evaluation of glycoproteins purified from adult and larval camel ticks (Hyalomma dromedarii) as a candidate vaccine
Amr E. El Hakim, Yasser E. Shahein*, Sobhy Abdel-Shafy, Amira M. K. Abouelella, Ragaa R. Hamed 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 7 243-249 (7 pages)
a single-step purification method with Con-A sepharose. The purified GLPs were evaluated as vaccines against camel tick infestation in rabbits. The rabbits received three intramuscular inoculations of GLPs (20 mg/animal) on days 0, 14, and 28. In the immunoblot analysis, Sera from the immunized rabbits recognized the native GLPs and other proteins from larval and adult H. dromedarii ticks along with those from other tick species such as Rhipicephalus sanguineus but not Ornithodoros moubata. The... -
Effects of hydrocortisone and aminophylline on the aggregation of equine platelets in vitro
Stefania Casella, Elisabetta Giudice, Claudia Giannetto, Simona Marafioti, Giuseppe Piccione* 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 3 215-219 (5 pages)
The purpose of this study was to evaluate in vitro the effects of hydrocortisone and aminophylline on adenosine diphosphate (ADP)-induced platelet aggregation in horses. Blood samples from 30 healthy Thoroughbred horses were collected by via jugular venipuncture to assess platelet aggregation. Platelet-rich and platelet-poor plasma were prepared from all samples by centrifugation and divided into three different aliquots. In the first aliquot, platelet aggregation was measured after platelet... -
Establishment of a standard operating procedure for predicting the time of calving in cattle
Dominik Streyl, Carola Sauter-Louis, Anna Braunert, Dorothee Lange, Frank Weber, Holm Zerbe 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 11 177-186 (10 pages)
Precise calving monitoring is essential for minimizing the effects of dystocia in cows and calves. We conducted two studies in healthy cows that compared seven clinical signs (broad pelvic ligaments relaxation, vaginal secretion, udder hyperplasia, udder edema, teat filling, tail relaxation, and vulva edema) alone and in combination in order to predict the time of parturition. The relaxation of the broad pelvic ligaments combined with teat filling gave the best values for predicting either... -
Dynamic analysis of Ca level during bovine oocytes maturation and early embryonic development
Su Li Liang, Qian Jun Zhao, Xiang Chen Li, Ya Ping Jin, Yi Peng Wang, Xiao Hua Su, Wei Jun Guan, Yue Hui Ma 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 5 133-142 (10 pages)
Mammalian oocyte maturation and early embryo development processes are Ca-dependent. In this study, we used confocal microscopy to investigate the distribution pattern of Ca and its dynamic changes in the processes of bovine oocytes maturation, in vitro fertilization (IVF), parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) embryo development. During the germinal vesicle (GV) and GV breakdown stage, Ca was distributed in the cortical ooplasm and throughout the oocytes from... -
Cloned calves derived from somatic cell nuclear transfer embryos cultured in chemically defined medium or modified synthetic oviduct fluid
Goo Jang*, So Gun Hong, Byeong Chun Lee* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 12 83-89 (7 pages)
Somatic cell nuclear transfer (SCNT) is considered to be a critical tool for propagating valuable animals. To determine the productivity calves resulting from embryos derived with different culture media, enucleated oocytes matured in vitro were reconstructed with fetal fibroblasts, fused, and activated. The cloned embryos were cultured in modified synthetic oviduct fluid (mSOF) or a chemically defined medium (CDM) and developmental competence was monitored. After 7 days of culturing, the... -
Expression of KA1 kainate receptor subunit in the substantia gelatinosa of the trigeminal subnucleus caudalis in mice
Seon Ah Park, Soo Joung Park, Seong Kyu Han* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 4호 4 299-304 (6 pages)
The KA1 kainate receptor (KAR) subunit in the substantia gelatinosa (SG) of the trigeminal subnucleus caudalis (Vc) has been implicated in the processing of nociceptive information from the orofacial region. This study compared the expression of the KA1 KAR subunit in the SG of the Vc in juvenile, prepubescent and adult mice. RT-PCR, Western blot and immunohistochemistry analyses were used to examine the expression level in SG area. The expression levels of the KA1 KAR subunit mRNA and protein... -
Molecular heterogeneity of plpE gene in Indian isolates of Pasteurella multocida and expression of recombinant PlpE in vaccine strain of P. multocida serotype B: 2
Ajay Pratap Singh, Satparkash Singh, Rajeev Ranjan*, Santosh Kumar Gupta, Vijendra Pal Singh, Bhaskar Sharma 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 8 227-233 (7 pages)
Outer membrane proteins of Pasteurella (P.) multocida have been known to be protective immunogens. Pasteurella lipoprotein E (PlpE) has been reported to be an important cross reactive outer membrane protein in P. multocida. The gene encoding the PlpE of P. multocida serotypes A: 3, B: 2 and D: 1 was amplified from the genomic DNA. The amplified products were cloned and the nucleotide sequence was determined. Sequence analysis of the recombinant clones revealed a single open reading frame of... -
Development of the body condition score system in Murrah buffaloes: validation through ultrasonic assessment of body fat reserves
Anitha Alapati, Sarjan Rao Kapa, Suresh Jeepalyam, Srinivasa Moorthy Patrapalle Rangappa, Kotilinga Reddy Yemireddy 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 1호 1 1-8 (8 pages)
subjective scoring method of evaluating the energy reserves of dairy animals to provide better understanding of biological relationships between body fat, milk production and reproduction. This method helps in adopting the optimum management practices to derive maximum production and maintain optimum health of the livestock. In this study, a new BCS system was developed for Murrah buffaloes. The skeletal check points were identified by studying the anatomical features and amount of fat reserves... -
Evaluation of a competitive ELISA for antibody detection against avian influenza virus
Dae Sub Song, Youn Jeong Lee, Ok Mi Jeong, Yong Joo Kim, Chan Hee Park, Jung Eun Yoo, Woo Jin Jeon, Jun Hun Kwon, Gun Woo Ha, Bo 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 4호 8 323-329 (7 pages)
Active serologic surveillance is necessary to control the spread of the avian influenza virus (AIV). In this study, we evaluated a commercially-available cELISA in terms of its ability to detect AIV antibodies in the sera of 3,358 animals from twelve species. cELISA detected antibodies against reference H1- through H15-subtype AIV strains without cross reactivity. Furthermore, the cELISA was able to detect antibodies produced following a challenge of the AIV H9N2 subtype in chickens, or... -
An outbreak of fatal hemorrhagic pneumonia caused by Streptococcus equi subsp. zooepidemicus in shelter dogs
Jae-Won Byun*, Soon-Seek Yoon, Gye-Hyeong Woo, Byeong Yeal Jung, Yi-Seok Joo 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 15 269-271 (3 pages)
An outbreak of fatal hemorrhagic pneumonia with 70∼ 90% morbidity and 50% mortality occurred in an animal shelter in Yangju, Gyeonggi Province, Korea. Clinically, the affected dogs showed severe respiratory distress within 48 h after arriving in the shelter. The dead were found mainly with nasal bleeding and hematemesis. At necropsy, hemothorax and hemorrhagic pneumonia along with severe pulmonary consolidation was observed, though histopathological analysis showed mainly hemorrhagic...


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