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Cytokine response in Balb/c mice infected with Francisellatularensis LVS and the Pohang isolate
Eun-Ju Kim, Sang-Hee Park, Young-Sill Choi, Soo-Kyoung Shim, Mi-Yeoun Park, Man Suck Park, Kyu-Jam Hwang 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 3호 12 309-316 (8 pages)
We investigated the immune response induced by the Francisella (F.)tularensis live vaccine strain (LVS) and the Pohang isolate. After the Balb/c mice were infected intradermally (i.d) with 2 × 10 cfu of F. tularensis LVS and Pohang, respectively, their blood and organs were collected at different times; 0, 3, 6, 24, 72, 96, 120 and 168 h after infection. Using these samples, RT-PCR and ELISA analysis were carried out for the comparative study of the cytokines, including TNF-a, INF-g, IL-2,... -
Antigenic diversity of Theileria major piroplasm surface protein gene in Jeju black cattle
Myung-Soon Ko, Kyoung-Kap Lee, Kyu-Kye Hwang, Byung-Sun Kim, Gui-Cheol Choi, Young-Min Yun* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 2호 5 155-160 (6 pages)
Piroplasms are tick-transmitted, intracellular, hemoprotozoan parasites that cause anorexia, fever, anemia, and icterus. Theileriosis is caused by Theileria sergenti and causes major economic losses in grazing cattle in Japan and Korea. In May 2003, we examined the antigenic diversity of the major piroplasm surface protein (MPSP) gene in 35 healthy Jeju black cattle that were born and raised at the National Institute of Subtropical Agriculture. On microscopic examination of Giemsa-stained blood... -
Collaborative control initiatives targeting zoonotic agents of alveolar echinococcosis in the northern hemisphere
Masao Kamiya* 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 4호 1 313-322 (10 pages)
Alveolar echinococcosis is one of the most important lethal zoonotic helminth infections in the northern hemisphere. Currently, the threat to public health is increasing, as evidenced by the rising prevalence rate of alveolar echinococcosis, as well as the invasion of urban areas by infected wild foxes. This threat is further increased due to the involvement of pet dogs, and probably cats, as emerging sources of infection. These increased threats to public health also have associated economic... -
Diversity of swine Bordetella bronchiseptica isolates evaluated by RAPD analysis and PFGE
Eun-Kyung Shin, Yeon-Soo Seo, Jeong Hee Han, Tae-Wook Hahn 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 10 65-73 (9 pages)
The degree of genetic diversity in 45 Bordetella (B.) bronchiseptica strains comprised of a vaccine strain (N = 1), reference strains (N = 3) and field isolates (N = 41) was evaluated using random amplified polymorphic DNA (RAPD) fingerprinting and pulsed-field gel electrophoresis (PFGE). Three candidate primers were selected for RAPD analysis after screening 20 random decamer oligonucleotides for their discriminatory abilities. The OPA-07, OPA-08 and OPA-18 primers yielded 10, 10, and 6... -
Primary structure of mature SAG1 gene of an Indonesian Toxoplasma gondii and comparison with other strains
Sri Hartati, Asmarani Kusumawati, Hastari Wuryastuti, J. Sri Widada 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 3호 9 263-270 (8 pages)
Toxoplasma gondii is a persistent protozoan parasite capable of infecting almost any warm-blooded vertebrates. SAG1 (p30) is the prototypic member of a superfamily of surface antigens called SRS (SAG1-related sequence). It constitutes the most abundant and predominant antigen. In this paper the primary structure of mature SAG1 gene of an Indonesian T. gondii isolate is described and sequence comparison is made with published sequence data of 7 other strains or isolates. Sequence comparison... -
Parentage testing of Thoroughbred horse in Korea using microsatellite DNA typing
Sun-young Lee, Gil-jae Cho 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 11 63-67 (5 pages)
The present study was to construct a parentage testing system for Thoroughbred (TB)horse.A total number of 1, 285 TB horse samples including 962 foals for parentage testing, 9 sires and 314 dams for individual identification were genotyped.Genomic DNA was extracted from 5 hair roots and genotyped by using 14 microsatellite markers (AHT4, AHT5, ASB2, ASB17, ASB23, CA425, HMS1, HMS3, HMS6, HMS7, HTG4, HTG10, LEX3 and VHL20). This method consisted of multiplexing PCR procedure and showed reasonable... -
TaqMan reverse transcription polymerase chain reaction for the detection of Japanese encephalitis virus
Dong-Kun Yang, Chang-Hee Kweon, Byoung-Han Kim, Seong-In Lim, Seong-Hee Kim, Jun-Hun Kwon, Hong-Ryul Han 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 10 345-351 (7 pages)
One step TaqMan reverse transcription polymerase chain reaction (RT-PCR) using TaqMan probe was developed for detection of Japanese encephalitis virus (JEV). Real-time RT-PCR was optimized to quantify JEV using the detection system (Rotor Gene 2000 detector) and dual-labeled fluorogenic probes. The gene specific labeled fluorogenic probe for the 3' non-translated region (3' NTR) was used to detect JEV. When the specificity of the assay using specific JEV primers was evaluated by testing three... -
Increased expression of osteopontin in the spinal cords of Lewis rats with experimental autoimmune neuritis
Changjong Moon, Taekyun Shin 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 2 289-293 (5 pages)
To investigate the pattern of expression of osteopontin (OPN) in tissues of the central nervous system (CNS) responding to peripheral immunological stimulation, the expression of OPN was studied in the spinal cord of rats with experimental autoimmune neuritis (EAN). In this model system, the sciatic nerves and spinal nerve roots are the target organs of EAN and the spinal cord is a remote organ that may be indirectly affected. OPN was constitutively expressed in some astrocytes adjacent to the... -
Effect of combined sodium hyaluronate and carboxymethyl cellulose on ocular surface in rat dry eye model
Jong-gab Moon , Sae-kwang Ku , Young-sam Kwon 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 6 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2016, 제 56권 제 3호 4 155-160 (6 pages)


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