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본문검색 결과만 보기
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Development and characterization of a potential diagnostic monoclonal antibody against capsid protein VP1 of the chicken anemia virus
Yi-Yang Lien, Chi-Hung Huang, Fang-Chun Sun, Shyang-Chwen Sheu, Tsung-Chi Lu, Meng-Shiunn Lee, Shu-Chin Hsueh, Hsi-Jien Chen, Me 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 11 73-79 (7 pages)
Chicken anemia virus (CAV) is an important viral pathogen that causes anemia and severe immunodeficiency syndrome in chickens worldwide. In this study, a potential diagnostic monoclonal antibody against the CAV VP1 protein was developed which can precisely recognize the CAV antigen for diagnostic and virus recovery purposes. The VP1 gene of CAV encoding the N-terminus-deleted VP1 protein, VP1Nd129, was cloned into an Escherichia (E.) coli expression vector. After... -
Induction of humoral responses to BHV-1 glycoprotein D expressed by HSV-1 amplicon vectors
Andrea Maria Blanc, Mabel Beatriz Berois, Lorena Magalí Tomé, Alberto L. Epstein, Juan Ramón Arbiza* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 9 59-65 (7 pages)
Herpes simplex virus type-1 (HSV-1) amplicon vectors are versatile and useful tools for transferring genes into cells that are capable of stimulating a specific immune response to their expressed antigens. In this work, two HSV-1-derived amplicon vectors were generated. One of these expressed the full-length glycoprotein D (gD) of bovine herpesvirus 1 while the second expressed the truncated form of gD (gDtr) which lacked the trans-membrane region. After evaluating gD expression in the infected... -
Cloning and characterization of a selenium-independent glutathione peroxidase (HC29) from adult Haemonchus contortus
Wei Sun, Xiaokai Song, Ruofeng Yan, Lixin Xu, Xiangrui Li* 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 8 49-58 (10 pages)
The complete coding sequence of Haemonchus (H.) contortus HC29 cDNA was generated by rapid amplification of cDNA ends in combination with PCR using primers targeting the 5´- and 3´-ends of the partial mRNA sequence. The cloned HC29 cDNA was shown to be 1,113 bp in size with an open reading frame of 507 bp, encoding a protein of 168 amino acid with a calculated molecular mass of 18.9 kDa. Amino acid sequence analysis revealed that the cloned HC29 cDNA contained the conserved catalytic triad and... -
Comparison of four diagnostic methods for detecting rabies viruses circulating in Korea
Dong-Kun Yang*, Eun-Kyung Shin, Yoon-I Oh, Kyung-Woo Lee, Chung-San Lee, Seo-Young Kim, Jeong-A Lee, Jae-Young Song 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 7 43-48 (6 pages)
It is essential to rapidly and precisely diagnose rabies. In this study, we evaluated four diagnostic methods, indirect fluorescent antibody test (FAT), virus isolation (VI), reverse transcriptase polymerase chain reaction (RT-PCR), and rapid immunodiagnostic assay (RIDA), to detect rabies in animal brain homogenates. Out of the 110 animal brain samples tested, 20 (18.2%) were positive for rabies according to the FAT. Compared to the FAT, the sensitivities of VI, RT-PCR, and RIDA were 100, 100,... -
The effect of conglutinin on production of reactive oxygen species in bovine granulocytes
Marta Dec*, Andrzej Wernicki, Andrzej Puchalski, Renata Urban-Chmiel, Adam Wa?ko 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 5 33-38 (6 pages)
Conglutinin is a high molecular-weight lectin originally detected in bovine serum. It belongs to the family of collectins that bind sugar residues in a Ca-dependent manner and are effector molecules in innate immunity. Conglutinin appears to play an important role in immune defense mechanisms, showing antiviral and antibacterial activities when tested in vivo and in vitro. The present study evaluated the effect of conglutinin on the respiratory bursts in bovine peripheral phagocytes. Using... -
Immunomodulatory effects of human amniotic membrane-derived mesenchymal stem cells
Jung Won Kang, Hye Cheong Koo*, Sun Young Hwang, Sung Keun Kang, Jeong Chan Ra, Moon Han Lee, Yong Ho Park* 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 4 23-31 (9 pages)
Human amniotic membrane-derived mesenchymal stem cells (hAM-MSCs) are capable of differentiating into several lineages and possess immunomodulatory properties. In this study, we investigated the soluble factor-mediated immunomodulatory effects of hAM-MSCs. Mitogen-induced peripheral blood mononuclear cell (PBMC) proliferation was suppressed by hAM-MSCs in a dose-dependent manner as well as hAM-MSC culture supernatant. Moreover, interferon-gamma and interleukin (IL)-17 production significantly... -
Prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle from farms in China
Fu Chen, Kehe Huang* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 3 15-22 (8 pages)
Fecal samples of 2,056 dairy cattle from 14 farms were collected in three geographical regions of China and stained using a modified acid-fast staining technique to identify Cryptosporidium oocysts. A total of 387 (18.82%) positive samples were identified and further analyzed by polymerase chain reaction (PCR) using primers designed to amplify DNA fragments from the small subunit ribosomal RNA. The PCR products were sequenced and the sequences were deposited in the GenBank database under... -
Elm tree bark extract inhibits HepG2 hepatic cancer cell growth via pro-apoptotic activity
Tae Myoung Kim, Sang-Kyung Shin, Tae-Wang Kim, So-Young Youm, Dae Joong Kim, Byeongwoo Ahn* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 2 7-13 (7 pages)
Control of inflammation is widely accepted as an important strategy for cancer chemoprevention. Anti-inflammatory effects of bark extracts of elm tree (BEE) have been amply reported. Therefore, BEE may be a good candidate cancer chemopreventive agent. Considering the high incidence of hepatic cancer and limited therapeutic approaches for treating this disease, it is important to develop liver cancer-specific chemopreventive agents. To evaluate the chemopreventive potential of BEE, we... -
Fast neutron irradiation deteriorates hippocampus-related memory ability in adult mice
Miyoung Yang, Hwanseong Kim, Juhwan Kim, Sung-Ho Kim, Jong-Choon Kim, Chun-Sik Bae, Joong-Sun Kim, Taekyun Shin, Changjong Moon* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 1 1-6 (6 pages)
Object recognition memory and contextual fear conditioning task performance in adult C57BL/6 mice exposed to cranial fast neutron irradiation (0.8 Gy) were examined to evaluate hippocampus-related behavioral dysfunction following acute exposure to relatively low doses of fast neutrons. In addition, hippocampal neurogenesis changes in adult murine brain after cranial irradiation were analyzed using the neurogenesis immunohistochemical markers Ki-67 and doublecortin (DCX). In the object... -
Cone beam computed tomography and intraoral radiography for diagnosis of dental abnormalities in dogs and cats
Marcello R. Roza*, Luiz Antonio F. Silva, Mauricio Barriviera, Alessandro L. Januário, Ana Cristina B. Bezerra, Maria Clori 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 12 387-392 (6 pages)
The development of veterinary dentistry has substantially improved the ability to diagnose canine and feline dental abnormalities. Consequently, examinations previously performed only on humans are now available for small animals, thus improving the diagnostic quality. This has increased the need for technical qualification of veterinary professionals and increased technological investments. This study evaluated the use of cone beam computed tomography and intraoral radiography as complementary...
