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261.

Prevalence and Clinical Characterization of Gastric Helicobacter Species Infection of Dogs and Cats in Korea

Cheol-Yong Hwang, Hong-Ryul Hanand Hwa-Young Youn 대한수의학회 Journal of Veterinary Science 12 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 2호 10 123-134 (12 pages)

Cheol-Yong Hwang, Hong-Ryul Hanand Hwa-Young Youn 저
...
- 4,100원
This study was carried out to evaluate the prevalence and clinical characterizations of gastric Helicobacter spp. infection of dogs and cats in Korea. The prevalence of Helicobacter spp. infection of dogs and cats determined by urease test was 78.4% and 64%, respectively, although Helicobacter genus-specific PCR assay showed that it was 82.3% and 84%. Urease mapping results based on urease test showed that total positive rate of tested tissues from clinically abnormal dogs was significantly...
262.

Time Course Study of Cytokine mRNA Expression in LPS-Stimulated Porcine Alveolar Macrophages

In-Soo Choi, Na-Ri Shin, Sung-Jae Shin, Deog-Yong Lee, Young-Wook Choand Han-Sang Yoo 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 2호 6 97-102 (6 pages)

In-Soo Choi, Na-Ri Shin, Sung-Jae Shin, Deog-Yong Lee, Young-Wook Choand Han-Sang Yoo 저
...
- 3,000원
The kinetics of cytokine mRNA expression was studied in porcine alveolar macrophages using an RT-PCR assay. The expression levels of IFN- gamma, IL-2, IL-4, IL-6, GM-CSF, IL-12 p35, and IL-12 p40 were examined after 2, 4, 14, 24, 48, and 72 h of incubation in unstimulated control and LPS-stimulated cells. The expression contents of IFN-gamma, IL-2, and IL-4 were not detected in both unstimulated and LPS-stimulated cells. On the other hand, the expression levels of IL-6, GM-CSF, and IL-12 in...
263.

Assessment of Porcine Reproductive and Respiratory Syndrome Virus RNA Load in Sera and Tissues during Acute Infection

Jin-Ho Shin and Thomas W. Molitor 대한수의학회 Journal of Veterinary Science 12 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 2호 4 75-86 (12 pages)

Jin-Ho Shin and Thomas W. Molitor 저
...
- 4,100원
Porcine reproductive and respiratory syndrome virus (PRRSV) RNA load in sera and tissues during acute phase of infection was evaluated using a PCR- based quantitative assay. More than 80% of infected pigs (21/25) showed the peak level of viral RNA concentrations in serum (up to 8.6 x 10(8) copies/ml) at day 5 postinfection (PI), and started to clear the virus from the systemic circulation thereafter. Regression analysis using the viral RNA concentrations in sera obtained from days 5 to 14 PI...
264.

Isolation of Subgroup J Avian Leukosis Virus in Korea

Haan-Woo Sung, Jae-Hong Kim, Sanjay Reddy and Aly Fadly 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 2호 3 71-74 (4 pages)

Haan-Woo Sung, Jae-Hong Kim, Sanjay Reddy and Aly Fadly 저
...
- 1,500원
Two subgroup J avian leukosis viruses (ALVs) were isolated from broiler breeder flocks, in which myeloid leukosis had occurred. The isolates could be classified as subgroup J ALV by the positive reaction in polymerase chain reaction (PCR) with primers specific for subgroup J ALV. Two isolates replicated in chicken embryo fibroblast (CEF) cells from the alv6 chicken line in which cells are resistant to subgroup A and E ALVs. In in vitro serum neutralization tests with other subgroup ALVs...
265.

Assessment of Replication and Virulence of Attenuated Pseudorabies Virus in Swine

T. J. Newby, D. P. Carter, K.-J. Yoon, M. W. Jackwood and P. A. Hawkins 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 2호 1 61-66 (6 pages)

T. J. Newby, D. P. Carter, K.-J. Yoon, M. W. Jackwood and P. A. Hawkins 저
...
- 3,000원
A nonclinical study was conducted to characterize the replication behavior of a modified live gE-deleted pseudorabies virus (PRV MS) in swine and potential for reversion to virulence after animal passages. Two to 3 week-old weaned pigs, negative for PRV, were maintained in isolation and challenged by intranasal instillation. For the first passage, 6 pigs were given 1 mL of PRV MS (10(7.3)TCID(50)/mL) and 2 were necropsied at 3, 4 and 5 days post-inoculation (PI). Brain and secondary lymphoid...
266.

Expression of Angiostatin Using DNA - Based Semliki Forest Virus Replicon

Hyun Soo Kim, Kwang Soon Shin, Jong Soo Lee, Young Ki Choi, Chul Joong Kim, Yong Soo Choi 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 1호 7 41-45 (5 pages)

Hyun Soo Kim, Kwang Soon Shin, Jong Soo Lee, Young Ki Choi, Chul Joong Kim, Yong Soo Choi 저
...
- 1,500원
Angiogenesis is recognized as a critical factor in the growth of tumor cells and plays a key role in the tumor metastasis. Recent studies for antiangiogenic substances are getting popular. The angiostatin, one of the antiangiogenic substances, leads to the increased apoptosis of the tumor cells by inhibiting the neovascularization of the tumor. The angiostatin was identified as the internal fragments of the plasminogen which has no antiangiogenic activity. By hydrolysis of the plasminogen, the...
267.

Characterization of the Recombinant Proteins of Porcine ircovirus Type 2 Field Isolate Expressed in the Baculovirus System

Yu Na Kim, Jin Hyun Kim, Kyoung Soo Kang, Young S. Lyoo 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 1호 4 19-23 (5 pages)

Yu Na Kim, Jin Hyun Kim, Kyoung Soo Kang, Young S. Lyoo 저
...
- 1,500원
Porcine circovirus (PCV) type2 was isolated using primary porcine kidney cells from lymph node of piglets with typical PMWS. The presence of the virus was identified by PCR using primers specific to PCV type2. The ORFs 1 and 2 were amplified by PCR using primers corresponding to the target genes of the PCV type 2. Cloned genes were inserted into the baculovirus expression vector and PCV recombinant proteins were expressed using baculovirus expression system. Recombinant protein expression was...
268.

Expression of Open Reading Frame 5 Protein of Porcine Reproductive and Respiratory Syndrome Virus Using Semliki Forest Virus Expression System

Hyun Soo Kim, Kwang Soon Shin, In Wook Hwang, Chul Joong Kim, Su Mi Kim, Hae Sun Jung 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 1호 3 13-18 (6 pages)

Hyun Soo Kim, Kwang Soon Shin, In Wook Hwang, Chul Joong Kim, Su Mi Kim, Hae Sun Jung 저
...
- 3,000원
The ORF5 gene encodes a major envelope glycoprotein (GP5), which is one of the three major proteins of porcine reproductive and respiratory syndrome virus (PRRSV). The GP5 protein has been known to be a 24.5-26 kDa N-glycosylated envelope protein. The GP5 is involved in inducing neutralizing antibodies. For this reason, the GP5 is primary candidate for the PRRSV subunit vaccine. To produce the native form of GP5 in mammalian cells, we have cloned the ORF5 gene from PRRSV CNV-1 into the Semliki...
269.

Cloning a new allele form of bovine TNF-a

Jongsam Ahn 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 2호 12 139-141 (3 pages)

Jongsam Ahn 저
...
- 1,500원
Although little is known on the function of gammadelta T lymphocytes, there is increasing evidence that gammadelta T lymphocytes are early responders and modulators of immune responses against pathogens and cytokines such as IL-2, IL-7, IL-15 and TNF-a. To study the role TNF-a on gammadelta T lymphocytes, we cloned bovine TNF-a. Sequence analysis revealed that a new allele form of bovine TNF-a was cloned which has 3 additional nucleotide sequences as well as 3 nucleotide substitutions compared...
270.

Studies of cocktail therapy with multiple cytokines for neoplasia or infectious disease of the dog I. cDNA cloning of canine IL-3 and IL-6

Il-seob Shin, Hye-ryon Kim, Myung-jin Nam1 and Hwa-young Youn 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 2호 8 115-120 (6 pages)

Il-seob Shin, Hye-ryon Kim, Myung-jin Nam1 and Hwa-young Youn 저
...
- 3,000원
This paper describes the cloning and sequence analysis of the cDNAs encoding the canine homologues of interleukin-3 (IL-3) and interleukin-6 (IL-6). The coding sequences for canine IL-3 and IL-6 were obtained by using the reverse transcription polymerase chain reaction (RT-PCR) with RNA harvested from canine peripheral blood mononuclear cells (PBMCs). Canine IL-3 cDNA includes a single open reading frame of 432 nucleotides, which encodes a 143 amino acid polypeptide and has 44.7, 42.4, 37 and...

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