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261.

Open Access : Specific and Sensitive Detection of Venturia nashicola, the Scab Fungus of Asian Pears, by Nested PCR

한국식물병리학회 The Plant Pathology Journal 7 Pages

한국식물병리학회 The Plant Pathology Journal 2013, 29권 4호 1 357-363 (7 pages)

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- 3,000원
a PCR-based technique was developed. DNA fingerprints of three closely related species, V. nashicola, V. pirina, and V. inaequalis, were obtained by random amplified polymorphic DNA (RAPD) analysis. Two RAPD markers specific to V. nashicola were identified by PCR, after which two pairs of sequence characterized amplified region (SCAR) primers were designed from the nucleotide sequences of the markers. The SCAR primer pairs, designated as D12F/D12R and E11F/E11R, amplified 535-bp and 525-bp DNA...
262.

Research Articles : Prevalence of Tobacco mosaic virus in Iran and Evolutionary Analyses of the Coat Protein Gene

한국식물병리학회 The Plant Pathology Journal 14 Pages

한국식물병리학회 The Plant Pathology Journal 2013, 29권 3호 5 260-273 (14 pages)

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- 4,300원
100%) among the Iranian isolates. Phylogenetic analysis of all known TMV CP genes showed three clades on the basis of nucleotide sequences with all Iranian isolates distinctly clustered in clade II. Analysis using the complete CP amino acid sequence showed one clade with two subgroups, IA and IB, with Iranian isolates in both subgroups. The nucleotide diversity within each subgroup was very low, but higher between the two clades. No correlation was found between genetic distance and geographical...
263.

Chemical and Biological Controls of Balloon Flower Stem Rots Caused by Rhizoctonia solani and Sclerotinia sclerotiorum

한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2012, 28권 2호 4 156-163 (8 pages)

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- 3,000원
Stem rots caused by Rhizoctonia solani and Sclerotinia sclerotiorum have been known as devastating diseases in balloon flower plants. Antifungal activities of four fungicides, azoxystrobin, polyoxin B, trifloxystrobin and validamycin A were evaluated in vitro, showing effective suppression with mycelial growth of the fungal isolates on PDA media. Efficacies of the four fungicides were also demonstrated in stem tissues of balloon flower plants against R. solani and S. sclerotiorum. A commercially...
264.

Research Notes : Multiplex PCR Assay for Simultaneous Detection of Korean Quarantine Phytoplasmas

한국식물병리학회 The Plant Pathology Journal 5 Pages

한국식물병리학회 The Plant Pathology Journal 2011, 27권 4호 10 367-371 (5 pages)

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- 1,500원
for each ``Ca. Phytoplasma`` species. In addition, ten important ``Ca. Phytoplasma`` species could be easily determined by recognizing band patterns specific for each phytoplasma species from three primer sets. Moreover, a high sensitivity of multiplex PCR for each primer set was observed for samples containing a low DNA concentration (10 ng/ul). This study provides the useful multiplex PCR assay as a convenient method to detect the presence of ten important quarantine phytoplasmas in Korea.
265.

Identification of Alternaria alternata as a Causal Agent for Leaf Blight in Syringa Species

한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2011, 27권 2호 4 120-127 (8 pages)

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- 3,000원
While many isolates of Alternaria alternata are common saprophytes on trees and shrubs, this study clearly demonstrated that A. alternata is a primary pathogen in lilac (Syringa sp.), causing a leaf-blight that affects different Syringa species. Isolates of Alternaria sp. were collected from leaf blight samples of lilacs in the field. The internal transcribed spacer (ITS) region and morphological characterization were used to identify lilac blight pathogen. Based on 100% ITS nucleotide sequence...
266.

Generation of Antibodies Against Rice stripe virus Proteins Based on Recombinant Proteins and Synthetic Polypeptides

한국식물병리학회 The Plant Pathology Journal 7 Pages

한국식물병리학회 The Plant Pathology Journal 2011, 27권 1호 7 37-43 (7 pages)

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- 3,000원
proteins in RSV, genes that code for NCP and NS3 proteins were cloned and subcloned into vector carrying His-tag protein and were expressed in E. coli. Of two recombinant proteins, only anti-NCP displayed stable hybridization signals in western blot analysis. Alternately, synthetic RSV polypeptides for CP, NCP, NS3 and NSvc4 we also generated and only antibodies against CP and NCP were very effective to detect RSV in both RSV infected rice and weed plants. However, antibodies against NS3 and NSv...
267.

Effect of Alternaria solani Exudates on Resistant and Susceptible Potato Cultivars from Two Different pathogen isolates

한국식물병리학회 The Plant Pathology Journal 6 Pages

한국식물병리학회 The Plant Pathology Journal 2011, 27권 1호 3 14-19 (6 pages)

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- 3,000원
in many countries around the world. Our previous screening of several Iranian potato cultivars found that significant variation in resistance phenotypes exists between two cultivars: resistant ``Diamond`` and susceptible ``Granula``. Our previous analysis of five different pathogen isolates also identified varying degrees of aggressiveness regardless of the host cultivar. Here, a bioassay was used to study the role of liquid culture exudates produced in vitro on pathogenicity and elicitation of...
268.

Specific PCR Detection of Four Quarantine Fusarium Species in Korea

한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2010, 26권 4호 16 409-416 (8 pages)

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- 3,000원
Fusarium species, a large group of plant pathogens, potentially pose quarantine concerns worldwide. Here, we focus on the development of a method for detecting four Fusarium species in quarantined plants in Korea: F. solani f. sp. cucurbitae, F. stilboides, F. redolens, and F. semitectum var. majus. Species-specific primers were designed from the nucleotide sequences of either the translation elongation factor-1 alpha (TEF1) gene or RNA polymerase II subunit (RPB2) gene. Two different primer...
269.

Cnidium officinale, Fungal disease, Fusarium solani, Fusarium wilt

Na-Kyeong Kim, Hyo-Jeong Lee, Tae-Ho Ryu, In-Sook Cho, Ho-Jong Ju, Rae-Dong Jeong 한국식물병리학회 식물병연구 5 Pages

한국식물병리학회 식물병연구 2021, 27권 2호 6 79-83 (5 pages)

Na-Kyeong Kim, Hyo-Jeong Lee, Tae-Ho Ryu, In-Sook Cho, Ho-Jong Ju, Rae-Dong Jeong 저
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- 1,500원
The aim of the present study was to develop a sensitive and specific detection method for the rapid detec- tion of apple scar skin viroid (ASSVd) in apple leaves. The resulting reverse transcription recombinase poly- merase amplification (RT-RPA) assay can be completed in 10 min at 42oC, is 10 times more sensitive than con- ventional reverse transcription polymerase chain reaction, and can specifically amplify ASSVd without any cross-reactivity with other common apple viruses, including apple...
270.

Development of Diagnostic Technology of Xylella fastidiosa Using Loop-Mediated Isothermal Amplification and PCR Methods

Suyoung Kim, Yujin Park, Gidon Kim 한국식물병리학회 식물병연구 7 Pages

한국식물병리학회 식물병연구 2021, 27권 1호 6 38-44 (7 pages)

Suyoung Kim, Yujin Park, Gidon Kim 저
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- 3,000원
capability of X. fastidiosa in the field, the loop-mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) assay were developed to mqsA gene of citrate-synthase (XF 1535) X. fastidiosa and evaluated for speci- ficity and sensitivity. Both assays were more robust than current published tests for detection of X. fastidiosa when screened against 16 isolates representing the four major subgroups of the bacterium from a range of host species. No cross reaction with DNA from...

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