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Multi-Homologous Recombination-Based Gene Manipulation in the Rice Pathogen Fusarium fujikuroi
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 3호 1 173-181 (9 pages)
gene disruption construct. This method utilizes DNA fragment fusion and requires only one PCR step and one reaction for construction. Using this strategy, a gene disruption construct for Fusarium cyclin C1 (FCC1 ), which is associated with fumonisin B1 biosynthesis, was successfully created and used for fungal transformation. In vivo and in vitro experiments using confirmed fcc1 mutants suggest that fumonisin production is closely related to disease symptoms exhibited by F. fujikuroi strain B14.... -
Stem Rot on Adzuki Bean (Vigna angularis) Caused by Rhizoctonia solani AG 4 HGI in China
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 1호 8 67-71 (5 pages)
on their morphology, and sequence and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analyses of the ribosomal DNA internal transcribed spacers (rDNA-ITS) region, the four isolates were identified as Rhizoctonia solani in anastomosis group (AG) 4 HGI. Pathogenicity tests showed that all isolates were strongly pathogenic to adzuki bean and resulted in serious wilt symptoms which was similar to observations in the fields. Additionally, the isolates infected several... -
An Acidic PATHOGENESIS-RELATED1 Gene of Oryza grandiglumis is Involved in Disease Resistance Response Against Bacterial Infection
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2014, 30권 2호 13 208-214 (7 pages)
Wild rice, Oryza grandiglumis shows hyper-resistance response to pathogen infection. In order to identify genes necessary for defense response in plants, we have carried out a subtractive hybridization coupled with a cDNA macroarray. An acidic PATHOGENESIS-RELATED1 (PR1) gene of the wild rice is highly identical to the acidic PR1 genes of different plant species. The OgPR1a cDNA has an apparent single open reading frame with a predicted molecular mass 40,621 Da and an isoelectic point of 5.14.... -
Evaluation of Watermelon Germplasm for Resistance to Phytophthora Blight Caused by Phytophthora capsici
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2013, 29권 1호 10 87-92 (6 pages)
This study was conducted to determine the Phytophthora rot resistance of 514 accessions of watermelon germplasm, Citrullus lanatus var lanatus. About 46% of the 514 accessions tested were collections from Uzbekistan, Turkey, China, U.S.A., and Ukraine. Phytophthora capsici was inoculated to 45-day-old watermelon seedlings by drenching with 5 ml of sporangial suspension (106 sporangia/ml). At 7 days after inoculation, 21 accessions showed no disease symptoms while 291 accessions of susceptible... -
Partial Biological and Molecular Characterization of Tomato yellow fruit ring virus Isolates from Potato
한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2012, 28권 4호 6 390-400 (11 pages)
Eight potato-producing provinces of Iran were surveyed during the growing seasons of 2004?2006 to detect the presence of Tomato yellow fruit ring virus (TYFRV), a tentative species in the genus Tospovirus. A total of 1,957 potato leaf samples were collected from plants with tospovirus-like symptoms of chlorotic or necrotic spots, chlorosis and necrosis. The samples were tested by enzyme-linked immunosorbent assay using TYFRVspecific antibodies. Among those tested, 498 samples (25.4%) were found... -
Research Notes : Simple and Rapid Detection of Potato Leafroll virus by Reverse Transcription Loop-mediated Isothermal Amplification
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 4호 13 385-389 (5 pages)
transcription loop-mediated isothermal amplification (RT-LAMP) method for the Potato leafroll virus (PLRV) was developed and compared with conventional reverse transcription polymerase chain reaction (RT-PCR) to address its advantages over RTPCR. RT-LAMP primers were designed from the open reading frame 3 (ORF3) sequence of PLRV. The RTLAMP reactions were conducted without or with a set of loop primers. By real-time monitoring using Turbimeter, the RT-LAMP (with loop primers) detects PLRV in... -
Occurrence of Tropical Race 4 of Fusarium oxysporum f. sp. cubense in Indonesia
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 3호 10 280-284 (5 pages)
Fusarium wilt of banana caused by Fusarium oxysporum f. sp. cubense is widespread in Indonesia. However, the distribution of tropical race 4 strains has not been well studied. Thirty nine isolates of F. oxysporum f. sp. cubense were collected from Java and 7 isolates were from Sumatera, Bangka, and Kalimantan. All isolates produced volatile odor when grown on steamed rice. These isolates were further tested for their vegetative compatibility with nitM testers of 20 reported vegetative... -
Cnidium officinale, Fungal disease, Fusarium solani, Fusarium wilt
Na-Kyeong Kim, Hyo-Jeong Lee, Tae-Ho Ryu, In-Sook Cho, Ho-Jong Ju, Rae-Dong Jeong 한국식물병리학회 식물병연구 5 Pages
한국식물병리학회 식물병연구 2021, 27권 2호 6 79-83 (5 pages)
is 10 times more sensitive than con- ventional reverse transcription polymerase chain reaction, and can specifically amplify ASSVd without any cross-reactivity with other common apple viruses, including apple stem grooving virus, apple stem pitting virus, and apple chlorotic leaf spot virus. The reliability of the RT-RPA assay was assessed, and the findings suggested that it can be successfully utilized to detect ASSVd in field-collected samples. The RT-RPA assay developed in the present study... -
Survival of Erwinia amylovora on Surfaces of Materials Used in Orchards
Hyun Ju Choi, Yeon Ju Kim, Yeon-Jeong Lim, Duck Hwan Park 한국식물병리학회 식물병연구 5 Pages
한국식물병리학회 식물병연구 2019, 25권 2호 6 89-93 (5 pages)
Fire blight disease caused by the bacterium, Erwinia amylovora, was observed in apple and pear orchards in Korea in 2015. Since then, it has spread, sometimes over long distances to other orchards. Therefore, we examined the ability of E. amylovora to survive in soils and on the surfaces of common materials such as T-shirts, wrist bands, pruning shears, and rubber boots by both conventional PCR (cPCR) and quantitative PCR (qPCR) methods. E. amylovora was detected in all materials tested in this... -
토마토 궤양병 신속 진단을 위한 Clavibacter michiganensis의 PCR 검출법
박미정(Mi-Jeong Park), 백창기(Chang-Gi Back), 박종한(Jong-Han Park) 한국식물병리학회 식물병연구 6 Pages
한국식물병리학회 식물병연구 2018, 24권 4호 14 342-347 (6 pages)


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