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Antiviral effect of dietary germanium biotite supplementation in pigs experimentally infected with porcine reproductive and respiratory syndrome virus
Bock-Gie Jung, Jin-A Lee, Bong-Joo Lee* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 4 135-141 (7 pages)
Germanium biotite (GB) is an aluminosilicate mineral containing 36 ppm germanium. The present study was conducted to better understand the effects of GB on immune responses in a mouse model, and to demonstrate the clearance effects of this mineral against Porcine reproductive and respiratory syndrome virus (PRRSV) in experimentally infected pigs as an initial step towards the development of a feed supplement that would promote immune activity and help prevent diseases. In the mouse model,... -
Expression and lytic efficacy assessment of the Staphylococcus aureus phage SA4 lysin gene
Anil Kumar Mishra*, Mayank Rawat, Konasagara Nagaleekar Viswas, Abhishek, Sujeet Kumar, Manjunatha Reddy 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 6 37-43 (7 pages)
of the SA4 phage was subsequently expressed in Escherichia coli. Recombinant LysSA4 induced the lysis of host bacteria in a spot inoculation test, indicating that the protein was expressed and functionally active. Furthermore, recombinant lysin was found to have lytic activity, albeit a low level, against mastitogenic Staphylococcus isolates of bovine origin. Data from the current study can be used to develop therapeutic tools for treating diseases caused by drug-resistant S. aureus strains. -
Molecular characterization of Japanese encephalitis virus strains prevalent in Chinese swine herds
Hao Zheng, Tongling Shan, Yu Deng, Chunqing Sun, Shishan Yuan, Yang Yin, Guangzhi Tong* 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 5 27-36 (10 pages)
Japanese encephalitis virus (JEV) is the leading cause of viral encephalitis in Asia and domestic pigs serve as the amplifying hosts. In the present study, the full genomic sequences of two JEV strains (HEN0701 and SH0601) isolated from pigs in China were determined and compared with other 12 JEV strains deposited in GenBank. These two strains had an 88.8% nucleotide sequence similarity and 97.9% deduced amino acid sequence homology. HEN0701 had high nucleotide sequence and high amino acid... -
Pathological characterization of TgElk mice injected with brain homogenate from elk with chronic wasting disease
Yong-Chul Jeon, Jin-Kyu Choi, Eun-Kyung Choi, Richard I. Carp, Yong-Sun Kim* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 4 21-26 (6 pages)
Chronic wasting disease (CWD) is classified as a transmissible spongiform encephalopathy or prion disease that affects cervids. CWD has been reported in 15 US states, two Canadian provinces, and in imported elk on several farms in Korea. This study was conducted to examine the molecular biological and pathogenic characteristics of a CWD-associated prion isolated in Korea. The epidemiological origin of this pathogen was also determined. Homozygous TgElk mice were infected with a CWD-affected elk... -
Comparative study of pandemic (H1N1) 2009, swine H1N1, and avian H3N2 influenza viral infections in quails
Aunyaratana Thontiravong, Suwarak Wannaratana, Rachod Tantilertcharoen, Duangduean Prakairungnamthip, Ranida Tuanudom, Jiroj Sas 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 4호 9 395-403 (9 pages)
Quail has been proposed to be an intermediate host of influenza A viruses. However, information on the susceptibility and pathogenicity of pandemic H1N1 2009 (pH1N1) and swine influenza viruses in quails is limited. In this study, the pathogenicity, virus shedding, and transmission characteristics of pH1N1, swine H1N1 (swH1N1), and avian H3N2 (dkH3N2) influenza viruses in quails was examined. Three groups of 15 quails were inoculated with each virus and evaluated for clinical signs, virus... -
Distribution of red-spotted grouper nervous necrosis virus (RGNNV) antigens in nervous and non-nervous organs of European seabass (Dicentrarchus labrax) during the course of an experimental challenge
Benjamin Lopez-Jimena, Esther Garcia-Rosado, Kim Dawn Thompson, Alexandra Adams, Carlos Infante, Juan Jose Borrego, Maria del Ca 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 4호 4 355-362 (8 pages)
Viral proteins were present throughout the experimental period in the retina (inner nuclear layer, ganglion layer, outer limiting membrane, and outer plexiform layer), brain (cerebellum and tectum opticum), and liver (hepatocytes and endothelial cells). These proteins were also observed in the renal tubular cells, white pulp of spleen, and in fibroblasts and cartilage of caudal fin. This is the first report of RGNNV proteins appearing in these organs, where the immunostaining was only detected... -
Protective effects of recombinant Brucella abortus Omp28 against infection with a virulent strain of Brucella abortus 544 in mice
Jeong Ju Lim, Dong Hyeok Kim, Jin Ju Lee, Dae Geun Kim, Wongi Min, Hu Jang Lee, Man Hee Rhee, Suk Kim* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 10 287-292 (6 pages)
The outer membrane proteins (OMPs) of Brucella (B.) abortus have been extensively studied, but their immunogenicity and protective ability against B. abortus infection are still unclear. In the present study, B. abortus Omp28, a group 3 antigen, was amplified by PCR and cloned into a maltose fusion protein expression system. Recombinant Omp28 (rOmp28) was expressed in Escherichia coli and was then purified. Immunogenicity of rOmp28 was confirmed by Western blot analysis with Brucella-positive... -
Exendin-4 improves resistance to Listeria monocytogenes infection in diabetic db/db mice
Hsien Yueh Liu, Chih-Yao Chung, Wen-Chin Yang, Chih-Lung Liang, Chi-Young Wang, Chih-Yu Chang, Cicero Lee-Tian Chang* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 5 245-252 (8 pages)
The incidence of diabetes mellitus is increasing among companion animals. This disease has similar characteristics in both humans and animals. Diabetes is frequently identified as an independent risk factor for infections associated with increased mortality. In the present study, homozygous diabetic (db/db) mice were infected with Listeria (L.) monocytogenes and then treated with the anti-diabetic drug exendin-4, a glucagon-like peptide 1 analogue. In aged db/db mice, decreased CD11b macrophage... -
Tissue distribution of sialic acid-linked influenza virus receptors in beagle dogs
Zhang-Yong Ning, Xin-Tao Wu, Yan-Fen Cheng, Wen-Bao Qi, Yu-Fu An, Heng Wang, Gui-Hong Zhang*, Shou-Jun Li* 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 1 219-222 (4 pages)
II and Sambucus nigra agglutinin. Both a-2,3- and a-2,6-sialic acid-linked receptors were detected in the endothelial cells of the respiratory tract and other organs. Endothelial cells of most gastrointestinal organs were negative for a-2,3-sialic acid-linked receptors in the dogs. Our results suggested that these canine organs may be affected by influenza virus infection. The findings from our study will also help evaluate the occurrence and development of influenza virus infections in dogs. -
Peste des petits ruminants virus detected in tissues from an Asiatic lion (Panthera leo persica) belongs to Asian lineage IV
Vinayagamurthy Balamurugan, Arnab Sen, Gnanavel Venkatesan, Vandana Bhanot, Vineeta Yadav, Veerakyathappa Bhanuprakash, Raj Kuma 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 13 203-206 (4 pages)
In this study, peste des petits ruminants virus (PPRV) was detected in frozen pooled tissue samples from a dead Asiatic lion (Panthera leo persica). The samples were negative for canine distemper virus and positive for PPRV nucleic acids when tested with one-step RT-PCR using the appropriate virus-specific primers. Subsequent amplification, cloning, and sequencing of the partial nucleocapsid, matrix, and fusion genes confirmed the presence of PPRV nucleic acid. Comparative sequence and...
