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전체 선택해제
총 265건 검색
본문검색 결과만 보기
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Development of in vitro produced porcine embryos according to serum types as macromolecule
Jungmin Son, Don Buddika Oshadi Malaweera, Eunsong Lee, Sangtae Shin, Jongki Cho 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 11 315-321 (7 pages)
This study was conducted to establish an in vitro maturation (IVM) system by selection of efficient porcine serum during porcine in vitro production. To investigate the efficient porcine serum (PS), different types of PS [newborn pig serum, prepubertal gilt serum (PGS), estrus sow serum, and pregnancy sow serum] were used to supplement IVM media with or without gonadotrophin (GTH) and development rates of parthenogenetic activation (PA) and in vitro fertilization (IVF) embryos were then... -
GC/MS analysis of high-performance liquid chromatography fractions from Sophora flavescens and Torilis japonica extracts and their in vitro anti-neosporal effects on Neospora caninum
Hun-Su Seo, Kyoung Hee Kim, Dae-Yong Kim, Bong-Kyun Park, Nam-Shik Shin, Jae-Hoon Kim, Heejeong Youn 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 2 241-248 (8 pages)
N. caninum was then assessed. Gas chromatography/ mass spectrometry (GC/MS) was used to identify and quantify specific anti-neosporal molecules in the herbal extracts. Almost all HPLC fractions of S. flavescens and T. japonica had higher levels of anti-neosporal activity compared to the not treated control. Active constituents of the extracts were sophoridane, furosardonin A, and tetraisopropylidene-cyclobutane in S. flavescens; 5,17-β-dihydroxy-de-A-estra-5,7,9,14-tetraene, furanodiene, and... -
Development and characterization of a potential diagnostic monoclonal antibody against capsid protein VP1 of the chicken anemia virus
Yi-Yang Lien, Chi-Hung Huang, Fang-Chun Sun, Shyang-Chwen Sheu, Tsung-Chi Lu, Meng-Shiunn Lee, Shu-Chin Hsueh, Hsi-Jien Chen, Me 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 11 73-79 (7 pages)
Chicken anemia virus (CAV) is an important viral pathogen that causes anemia and severe immunodeficiency syndrome in chickens worldwide. In this study, a potential diagnostic monoclonal antibody against the CAV VP1 protein was developed which can precisely recognize the CAV antigen for diagnostic and virus recovery purposes. The VP1 gene of CAV encoding the N-terminus-deleted VP1 protein, VP1Nd129, was cloned into an Escherichia (E.) coli expression vector. After... -
Evaluation of IGF-I levels and serum protein profiles of diabetic cats and dogs
Gulay Ciftci*, Gul Fatma Yarim 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 4 325-331 (7 pages)
In this study, we measured the insulin-like growth factor (IGF)-I levels and evaluated the serum protein profiles of diabetic, insulin-treated, and healthy cats and dogs. The total IGF-I concentrations were 33.74 ± 3.4 ng/mL for normal, 25.8 ± 4.5 ng/mL for diabetic, and 180.4 ± 31.4 ng/mL for insulin-treated cats. IGF-I concentrations were 46.4 ± 6.6 ng/mL for normal, 25.1 ± 4.1 ng/mL for diabetic, and 303.0 ± 61.3 ng/mL for insulin-treated dogs. Total serum protein profiles were analyzed... -
In vivo metabolism of Talosin A, new isoflavonol glycoside from Kitasatospora kifunensis, in rats
Jong-Hwan Lim, In-Bae Song, Youn-Hwan Hwang, Myoung-Seok Kim, Jong-Woo Kim, Jin-Yong Kim, Joo Won Suh, Hyo-In Yun 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 2 115-120 (6 pages)
The isoflavonol glycoside Talosin A, genistein (GT)-7-a-L-6- deoxy talopyranose (GT-Tal), was first isolated from the culture broth of Kitasatospora kifunensis MJM341. The aim of the present study was to evaluate the oral absorption and metabolism of the newly isolated isoflavonol glycoside, GT-Tal compared to genistin (GT-7-O-b-D-glucopyranoside; GT- Glu). Free GT-Glu and GT-Tal could not be detected prior to enzymatic hydrolysis of the corresponding conjugates in rat plasma. Following oral... -
Molecular heterogeneity of plpE gene in Indian isolates of Pasteurella multocida and expression of recombinant PlpE in vaccine strain of P. multocida serotype B: 2
Ajay Pratap Singh, Satparkash Singh, Rajeev Ranjan*, Santosh Kumar Gupta, Vijendra Pal Singh, Bhaskar Sharma 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 8 227-233 (7 pages)
1,017 bp encoding a protein with a calculated molecular mass of 37.829 kDa, 37.389 kDa and 37.965 kDa for serotypes A: 3, B: 2 and D: 1 respectively. The comparison of the plpE sequence in different capsular types revealed a high degree (＞90%) of homology. Furthermore, the plpE gene of Haemorhhagic septicaemia causing serotype (B: 2) was expressed in E. coli and recombinant PlpE was strongly immunostained by antiserum against whole cell antigen, indicating that the protein is expressed in vivo. -
Effect of multi-planar CT image reformatting on surgeon diagnostic performance for localizing thoracolumbar disc extrusions in dogs
Jason B. King, Jeryl C. Jones, John H. Rossmeisl Jr*, Tisha A. Harper, Otto I Lanz, Stephen R. Werre 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 8 225-232 (8 pages)
Accurate pre-operative localization and removal of disc material are important for minimizing morbidity in dogs with thoracolumbar disc extrusions. Computed tomography (CT) is an established technique for localizing disc extrusions in dogs, however the effect of multi-planar reformatting (MPR) on surgeon diagnostic performance has not been previously described. The purpose of this study was to test the effect of MPR CT on surgeon diagnostic accuracy, certainty and agreement for localizing... -
Evaluation of serum chondroitin sulfate and hyaluronan: biomarkers for osteoarthritis in canine hip dysplasia
Korakot Nganvongpanit, Akanit Itthiarbha, Siriwan Ong-Chai, Prachya Kongtawelert 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 3호 13 317-326 (10 pages)
Hip dysplasia (HD) is one of the most important bone and joint diseases in dogs. Making the radiographic diagnosis is sometime possible when the disease has markedly progressed. Chondroitin sulfate (CS) and hyaluronan (HA) are the most important cartilage biomolecules that are elevated in the serum taken from dogs with osteoarthritis. The serum CS and HA can be detected by an ELISA technique, with using monoclonal antibodies against CS epitope 3B3 and WF6 and the HA chain as the primary... -
Plasma haptoglobin and immunoglobulins as diagnostic indicators of deoxynivalenol intoxication
Eun-Joo Kim, Sang-Hee Jeong, Joon-Hyoung Cho, Hyun-Ok Ku, Hyun-Mi Pyo, Hwan-Goo Kang, Kyoung-Ho Choi 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 3호 5 257-266 (10 pages)
- Time-of-Flight/ Mass Spectrometry (TOF/MS) and the immunoglobulins (Igs) G, A, M and E in the serum were investigated. 11.7 kDa protein was significantly highly expressed according to DON administration and this protein was purified by employing a methyl ceramic HyperD F column with using optimization buffer for adsorption and desorption. The purified protein was identified as a haptoglobin precursor by peptide mapping with using LC/Q-TOF/MS and MALDI-TOF/MS and this was confirmed by western... -
Comparison of cardiac function and coronary angiography between conventional pigs and micropigs as measured by multidetector row computed tomography
Young Keun Ahn, Jung Min Ryu, Hea Chang Jeong, Yun Hyeon Kim, Myung Ho Jeong, Min Young Lee, Sang Hun Lee, Jae Hong Park, Seung 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 2호 1 121-126 (6 pages)
Pigs are the most likely source animals for cardiac xenotransplantation. However, an appropriate method for estimating the cardiac function of micropigs had not been established. Computed tomography (CT) analysis aimed at estimating cardiac function and assessing the coronary arteries has not been carried out in micropigs. This study determined the feasibility of evaluating cardiac function in a micropig model using multidetector row computed tomography (MDCT) and compared the cardiac function...
