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21.

Complete Genome Sequencing and Infectious cDNA Clone Construction of Soybean Mosaic Virus Isolated from Shanxi

Defu Wang, Liyan Cui, Li Zhang, Zhennan Ma, Yanbing Niu 한국식물병리학회 The Plant Pathology Journal 11 Pages

한국식물병리학회 The Plant Pathology Journal 2021, 37권 2호 7 162-172 (11 pages)

Defu Wang, Liyan Cui, Li Zhang, Zhennan Ma, Yanbing Niu 저
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- 4,000원
3,067 aa. SMV-SX and SMV-XFQ008 had the highest nucleotide and ami- no acid sequence identities of 97.03% and 98.50%, re- spectively. A phylogenetic tree indicated that SMV-SX and SMV-XFQ018 were clustered together, sharing the closest relationship. We then constructed a pSMV-SX infectious cDNA clone by Gibson assembly technology and used this clone to inoculate soybean and Ailanthus altissima; the symptoms of these hosts were similar to those caused by the virus isolated from natural infected...
22.

The Detection of Plant Viruses in Korean Ginseng (Panax ginseng) through RNA Sequencing

Hong-Kyu Lee, So-Yeon Kim, Hee-Ji Yang, Da-Som Lee, Boram Kwon, Dong-Yun Lee, Jonghee Oh, Su-Heon Lee 한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2020, 36권 6호 12 643-650 (8 pages)

Hong-Kyu Lee, So-Yeon Kim, Hee-Ji Yang, Da-Som Lee, Boram Kwon, Dong-Yun Lee, Jonghee Oh, Su-Heon Lee 저
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- 3,000원
A total of 656 leaf samples were combined into one and total RNA was extracted from the polled sample, using RNA sequencing (RNA-Seq), a metatranscriptome analysis of the plant virome was conducted. The virus present in Panax ginseng was confirmed by reverse transcription polymerase chain reaction (RT-PCR) assay using virus-specific primers. In RNA-Seq data analysis, the multiplication protein of four viral contigs including Aristotelia chilensis virus 1 (AcV1), Turnip mosaic virus (TuMV),...
23.

Characterization of a Strain of Malva Vein Clearing Virus in Alcea rosea via Deep Sequencing

Defu Wang, Liyan Cui, Yanni Pei, Zhennan Ma, Shaofei Shen, Dandan Long, Lingyu Li, Yanbing Niu 한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2020, 36권 5호 9 468-475 (8 pages)

Defu Wang, Liyan Cui, Yanni Pei, Zhennan Ma, Shaofei Shen, Dandan Long, Lingyu Li, Yanbing Niu 저
...
- 3,000원
development of Alcea rosea. It was first reported in Germany in 1957, but its complete genome sequence data are still scarce. In the present work, A. rosea leaves with vein-clearing and mosaic symptoms were sampled and analyzed with small RNA deep sequencing. By denovo assembly the raw sequences of virus-derived small interfering RNAs (vsiRs) and whole genome amplification of malva vein cleaning virus SX strain (MVCVSX) by specific primers targeting identified contig gaps, the full-length genome...
24.

De novo Genome Assembly and Single Nucleotide Variations for Soybean Mosaic Virus Using Soybean Seed Transcriptome Data

한국식물병리학회 The Plant Pathology Journal 10 Pages

한국식물병리학회 The Plant Pathology Journal 2017, 33권 5호 5 478-487 (10 pages)

저
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- 3,000원
examined single nucleotide variations (SNVs) for SMVs in the soybean seed transcriptome revealing 780 SNVs, which were evenly distributed on the SMV genome. Four SNVs, C-U, U-C, A-G, and G-A, were frequently identified. This result demonstrated the quasispecies variation of the SMV genome. Taken together, this study carried out bioinformatics analyses to identify viruses using soybean transcriptome data. In addition, we demonstrated the application of soybean transcriptome data for virus genome...
25.

Characterization of the Rosellinia necatrix Transcriptome and Genes Related to Pathogenesis by Single-Molecule mRNA Sequencing

한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2017, 33권 4호 2 362-369 (8 pages)

저
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- 3,000원
of R. necatrix (KACC 40445 strain) using PacBio single-molecule sequencing technology. After annotation of the unigene sets, we selected 14 cell cycle-related genes, which are likely either positively or negatively involved in hyphal growth by cell cycle control. The expression of the selected genes was further compared between two strains that displayed different growth rates on nutritional media. Furthermore, we predicted pathogen-related effector genes and cell wall-degrading enzymes from the...
26.

Methylome Analysis of Two Xanthomonas spp. Using Single-Molecule Real-Time Sequencing

한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2016, 32권 6호 2 500-507 (8 pages)

저
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- 3,000원
Single-molecule real-time (SMRT) sequencing allows identification of methylated DNA bases and methylation patterns/motifs at the genome level. Using SMRT sequencing, diverse bacterial methylomes including those of Helicobacter pylori, Lactobacillus spp., and Escherichia coli have been determined, and previously unreported DNA methylation motifs have been identified. However, the methylomes of Xanthomonas species, which belong to the most important plant pathogenic bacterial genus, have not been...
27.

Deep Sequencing Analysis of Apple Infecting Viruses in Korea

한국식물병리학회 The Plant Pathology Journal 11 Pages

한국식물병리학회 The Plant Pathology Journal 2016, 32권 5호 8 441-451 (11 pages)

저
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- 4,000원
Deep sequencing has generated 52 contigs derived from five viruses; Apple chlorotic leaf spot virus (ACLSV), Apple stem grooving virus (ASGV), Apple stem pitting virus (ASPV), Apple green crinkle associated virus (AGCaV), and Apricot latent virus (ApLV) were identified from eight apple samples showing small leaves and/or growth retardation. Nucleotide (nt) sequence identity of the assembled contigs was from 68% to 99% compared to the reference sequences of the five respective viral genomes....
28.

Taxonomic Position of Korean Isolates of Rhizoctonia solani Based on RAPD and ITS Sequencing of Ribosomal DNA

한국식물병리학회 The Plant Pathology Journal 7 Pages

한국식물병리학회 The Plant Pathology Journal 2010, 26권 1호 12 83-89 (7 pages)

저
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- 3,000원
Taxonomic position of 46 Korean isolates of Rhizoctonia solani which were classified into nine intraspecific groups by anastomosis and cultural characteristics was analyzed by randomly amplified polymorphic DNA (RAPD) and sequence analyses of the internal transcribed spacer (ITS) regions of ribosomal DNA. All the isolates within each group showed highly similar band patterns in RAPD. The ITS regions of the isolates within the same groups showed a high level of sequence similarity above 96.0%...
29.

The Complete Nucleotide Sequence of a Korean Isolate Bean yellow mosaic virus from Freesia sp. and Comparison to Other Potyviruses

한국식물병리학회 식물병연구 7 Pages

한국식물병리학회 식물병연구 2013, 19권 2호 1 77-83 (7 pages)

저
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- 3,000원
30.

Cloning and Sequencing of Coat Protein Gene of the Korean Isolate of Rice stripe virus

한국식물병리학회 The Plant Pathology Journal 3 Pages

한국식물병리학회 The Plant Pathology Journal 2004, 20권 4호 16 313-315 (3 pages)

저
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- 1,500원
isolate of Rice stripe virus (RSV-Kr) was cloned and its nucleotide sequence was determined. Total RNA was extracted from infected leaves and RSV viral RNA was detected by using RT-PCR with specific primer of coat protein gene. The result of RT-PCR showed a specific band. Purified RT-PCR products of coat protein gene were ligated into the pGEM-T Easy plasmid vector and cloned cDNA was obtained for nucleotide sequence determination. Coat protein gene of RSV-Kr consisted of 969 bp long encoding a...

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