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A novel mycotoxin purification system using magnetic nanoparticles for the recovery of aflatoxin B1 and zearalenone from feed
Hyun-Jung Kim, Sung-Hee Kim, Jin-Kyu Lee, Cheong-Up Choi, Hee-Soo Lee, Hwan-Goo Kang*, Sang-Ho Cha* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 4호 5 363-369 (7 pages)
novel tool for purifying two mycotoxins, aflatoxin B1 (AFB1) and zearalenone (ZEN), in feed. This system utilized monoclonal antibodies (mAbs) against AFB1 and ZEN, and magnetic nanoparticles (MNPs). Among ten MNPs with different diameters and functional groups, a 100-nm diameter MNP (fMA) conjugated to an amine group (-NH2) was found to be optimum for coupling with mAbs. The optimal mAb concentrations for coupling to the fMA along with mycotoxin purification capacities of the fMA-mAb conjugates... -
Characterization of the Recombinant Proteins of Porcine ircovirus Type 2 Field Isolate Expressed in the Baculovirus System
Yu Na Kim, Jin Hyun Kim, Kyoung Soo Kang, Young S. Lyoo 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 1호 4 19-23 (5 pages)
antiserum to PCV. ORF1 gene expressed two proteins with approximately 17 kDa and 31 kDa proteins in the baculovirus system. Recombinant protein of the ORF2 was similar to that of the native virus except minor bands with different molecular weight were detected. Recombinant protein expressed in the baculovirus system showed at least two glycosylation sites based on the tunicamycin treatment. Recombinant protein of the ORF2 assembled virus-like particle in recombinant virus infected insect cells. -
Expression of Open Reading Frame 5 Protein of Porcine Reproductive and Respiratory Syndrome Virus Using Semliki Forest Virus Expression System
Hyun Soo Kim, Kwang Soon Shin, In Wook Hwang, Chul Joong Kim, Su Mi Kim, Hae Sun Jung 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 1호 3 13-18 (6 pages)
form of GP5 in mammalian cells, we have cloned the ORF5 gene from PRRSV CNV-1 into the Semliki Forest virus (SFV)-based expression vector, resulting in recombinant pSFV-ORF5. By the infection with recombinant pSFV-ORF5 to BHK-21 cells, the GP5 expression was confirmed by immunocytochemistry and immunoblotting assay. The recombinant virus particle harboring ORF5 gene was infectious to BHK-21 and MARC-145. The RNA synthesis and expression of GP5 in the infected cell was also confirmed by RT-PCR. -
Identification of two cytopathogenic agents, Mycoplasma hyorhinis and mammalian orthoreovirus 3 based on modified particle associated nucleic acids PCR
Hye Kwon Kim , Hyoung Joon Moon , Seong Jun Park , Se Mi Rho , Jae Yeon Han , Van Giap Nguyen , Bong Kyun Park 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 9 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2011, 제 51권 제 2호 7 129-137 (9 pages)
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Regulatory Mechanisms of Angiotensin II on the Na+/H+ Antiport System in Rabbit Renal Proximal Tubule Cells. I. Stimulatory Effects of ANG II on Na+ Uptake
HoJaeHan, HyunJuKoh, SooHyunPark 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 11 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1997, Vol.1 No.4 8 413-423 (11 pages)
The importance of the kidney in the development of hypertension was first demonstrated by Goldblatt and his colleagues more than fifty years ago. Many hormones and other regulatory factors have been proposed to play a major role in the development of hypertension. Among these factors angiotensin II (ANG II) is closely involved in renal hypertension development since it directly regulates Na+ reabsorption in the renal proximal tubule. Thus the aim of the present study was to examine signaling... -
Optimization of a Virus-Induced Gene Silencing System with Soybean yellow common mosaic virus for Gene Function Studies in Soybeans
한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 2호 5 112-122 (11 pages)
a new VIGS system derived from the Soybean yellow common mosaic virus (SYCMV). Here, we investigated several environmental and developmental factors to improve the efficiency of a SYCMV-based VIGS system to optimize the functional analysis of the soybean. Following SYCMV: Glycine max-phytoene desaturase (GmPDS) infiltration, we investigated the effect of photoperiod, inoculation time, concentration of Agrobacterium inoculm, and growth temperature on VIGS efficiency. In addition, the relative... -
Impact of single metal oxide and coal ash on secondary ultrafine particle generation through SO2 and NO2 heterogeneous reaction
Ru-Jiao Song, Zi-Xiang Xu, Joo-Chang Park, Li-Hua Xu, Hyung-Taek Kim 한국열환경공학회 열환경공학 19 Pages
한국열환경공학회 열환경공학 2020, 제 15권 1호 4 28-46 (19 pages)
Particulate matter research is of great significance in solving atmospheric environment problems. The heterogeneous reaction of mixed gas on different mineral oxides and coal ash was studied based on a laboratory-built aerosol generating reactor. The product formed on particles was investigated by ion chromatography (IC). SO2 and NO2 could react on oxides to form secondary particles and the order of reactivity of mineral oxides under laboratory reaction conditions is: Al2O3>MgO>CaO>>SiO2, Fe2O3.... -
Evaluation of Ash Deposition Behavior and Fine Particle Formation from Combustion of Low-rank Coal Impregnated with Molasses in DTF
Zi-Xiang Xu, Joo-Chang Park, Hyung-Taek Kim 한국열환경공학회 열환경공학 9 Pages
한국열환경공학회 열환경공학 2019, 제 14권 2호 6 40-48 (9 pages)
a series of air pollutions. Thus, the investigation of ash deposition and fine particle formation is a significant topic to promote heat exchanger efficiency and protect the environment. In this study, three fuels, Berau coal, Berau coal impregnate with 10wt% and 20wt% molasses were selected. Experiments are carried out in drop tube furnace (DTF) under the combustion temperature of 1300°C and the ash probe temperature of 800°C with access air. The fuel is fed at a rate of 0.45g/min for 10min.... -
Numerical prediction for the performance of a floating-type breakwater by using a two-dimensional particle method
Lee. Byung-Hyuk, Hwang. Sung-Chul, Nam. Jung-Woo, Park. Jong-Chun 한국해양공학회 International journal of ocean system engineering 9 Pages
한국해양공학회 International journal of ocean system engineering 2011, Vol.1 No.1 37-45 (9 pages)


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