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Activation of autophagy flux by Ophiopogonin B downregulates cellular FLICE–like inhibitory protein and enhances TRAIL-induced apoptosis
Mohammad Rasheduzzaman, Jeong Min Hong, Uddin Nazim, Hong hua Yin, You Jin Lee, Jae Won Seol, Sang Youel Park 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 238 340-341 (2 pages)
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Analysis of protein expression in Brucella abortus mutants with different growth rates by two-dimensional gel electrophoresis and LC-MS/MS peptide analysis
Woo Bin Park, Young Bin Im, Soojin Shim, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 16 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 8 216-231 (16 pages)
mutant and wild-type B. abortus were purified and subjected to two-dimensional gel electrophoresis. Thirty protein spots of each mutant with expression increases or decreases were selected; those with a change of more than 2-fold were compared with the wild-type. Selected spots underwent liquid chromatography tandem mass spectrometry for peptide analysis. DnaK and ClpB, involved in protein aggregation, increased. SecA and GAPDH, associated with energy metabolism, decreased in some mutants with a... -
Polymorphism in the serotonin transporter protein gene in Maltese dogs with degenerative mitral valve disease
Chang-Min Lee, Jae-Ik Han, Min-Hee Kang, Seung-Gon Kim, Hee-Myung Park 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 16 129-135 (7 pages)
five nonsynonymous genetic variations, were detected in five exons. Protein structure and function of the five nonsynonymous genetic variations were predicted. Three of the five polymorphisms were predicted to be probable causes of damage to protein function and confirmed by protein structure model verification. This study identified six polymorphisms of the SERT gene in Maltese dogs with DMVD, suggesting an association between the SERT gene and canine DMVD. This is the first study of SERT... -
Coactosin-like protein 1 inhibits neuronal migration during mouse corticogenesis
Guohong Li, Yupeng Yin, Jiong Chen, Yanle Fan, Juhong Ma, Yingxue Huang, Chen Chen, Pengxiu Dai, Shulin Chen, Shanting Zhao 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 4 21-26 (6 pages)
Coactosin-like protein 1 (Cotl1), a member of the actin-depolymerizing factor (ADF)/cofilin family, was first purified from a soluble fraction of Dictyostelium discoideum cells. Neuronal migration requires cytoskeletal remodeling and actin regulation. Although Cotl1 strongly binds to F-actin, the role of Cotl1 in neuronal migration remains undescribed. In this study, we revealed that Cotl1 overexpression impaired migrationof both early- and late-born neurons during mouse corticogenesis.... -
Downregulation of cellular prion protein inhibited the proliferation and invasion and induced apoptosis of Marek’s disease virus-transformed avian T cells
Xuerui Wan, Runxia Yang, Guilin Liu, Manling Zhu, Tianliang Zhang, Lei Liu, Run Wu* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 6 171-178 (8 pages)
Cellular prion protein (PrP) is ubiquitously expressed in the cytomembrane of a considerable number of eukaryotic cells. Although several studies have investigated the functions of PrP in cell proliferation, cell apoptosis, and tumorigenesis of mammals, the correlated functions of chicken PrP (chPrP) remain unknown. In this study, stable chPrP-downregulated Marek’s disease (MD) virus-transformed avian T cells (MSB1-SiRNA-3) were established by introducing short interfering RNA (SiRNA) targeting... -
Molecular characterization of duck enteritis virus CHv strain UL49.5 protein and its colocalization with glycoprotein M
Meng Lin, Renyong Jia*, Mingshu Wang, Xinghong Gao, Dekang Zhu, Shun Chen, Mafeng Liu, Zhongqiong Yin, Yin Wang, Xiaoyue Chen, A 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 7 389-398 (10 pages)
constructed and used to transfect duck embryo fibroblasts. Next, the recombinant plasmid pDsRed1-N1/ glycoprotein M (gM) was produced and used for co-transfection with the pEGFP-C1/pUL49.5 plasmid to determine whether DEV pUL49.5 and gM (a conserved protein in herpesviruses) colocalize. DEV pUL49.5 was thought to be an envelope glycoprotein with a signal peptide and two transmembrane domains. This protein was also predicted to localize in the cytoplasm and endoplasmic reticulum with a probabilit... -
Tissue-specific expression of the NOD-like receptor protein 3 in BALB/c mice
Zhiqiang Huang, Meng Yu, Shuang Tong, Kun Jia, Rongchang Liu, Heng Wang, Shoujun Li*, Zhangyong Ning* 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 1 173-177 (5 pages)
of pathogen-associated molecular patterns, such as NOD-like receptors. The NOD-like receptor protein 3 (NLRP3) inflammasome is involved in induction of the pro- inflammatory cytokine, IL-1β, and subsequent inflammatory responses. NLRP3 inflammasome plays important roles in the inflammatory and innate immune responses associated with autoimmune/inflammatory syndrome. However, analysis of the tissue distribution and expression profiles in BALB/c mice is still incomplete. In this study, we... -
A simplified one-step nuclear transfer procedure alters the gene expression patterns and developmental potential of cloned porcine embryos
Sang Kyu Park, Sangho Roh*, Jong-Im Park* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 8 73-80 (8 pages)
Various somatic cell nuclear transfer (SCNT) techniques for mammalian species have been developed to adjust species-specific procedures to oocyte-associated differences among species. Species-specific SCNT protocols may result in different expression levels of developmentally important genes that may affect embryonic development and pregnancy. In the present study, porcine oocytes were treated with demecolcine that facilitated enucleation with protruding genetic material. Enucleation and donor... -
Development of monoclonal antibodies against the abnormal prion protein isoform (PrP) associated with chronic wasting disease (CWD)
Hyun-Jeong Jeong, Nak-Hyung Lee, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Kun-Ho Seo, Dong-Woon Kim, Yong-Sun Kim, In-So 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 4호 14 429-432 (4 pages)
Monoclonal antibodies (mAbs) specific for the abnormal prion protein isoform (PrP) are indispensable for diagnosing chronic wasting disease (CWD). In this study, eight mAbs were developed by immunizing PrP knockout mice with recombinant elk PrP and an immunogenic PrP peptide. The reactivity of the mAbs to recombinant PrP and the PrP peptide was measured, and their isotypes were subsequently determined. Among them, four mAbs (B85-05, B85-08, B85-12, and B77-75) were shown by Western blotting to...


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