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Rapid visual detection of Mycobacterium avium subsp. paratuberculosis by recombinase polymerase amplification combined with a lateral flow dipstick
Guimin Zhao, Hongmei Wang, Peili Hou, Chengqiang He, Hongbin He 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 10 242-250 (9 pages)
of domestic and wild ruminants. However, widespread point-of-care testing is infrequent due to the lack of a robust method. The isothermal recombinase polymerase amplification (RPA) technique has applied for rapid diagnosis. Herein, RPA combined with a lateral flow dipstick (LFD) assay was developed to estimate DNA from Mycobacterium avium subsp. paratuberculosis. First, analytical specificity and sensitivity of the RPA-nfo primer and probe sets were assessed. The assay successfully detected M.... -
Minimal systems analysis of mitochondria-dependent apoptosis induced by cisplatin
Ji-YoungHong, KenjirouHara, Jun-WooKim, EisukeF.Sato, EunBoShim, Kwang-HyunCho 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 12 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2016, Vol.20 No.4 6 367-378 (12 pages)
comprehensive approach to delineate the biological interactions of the molecules is required. However, quantitative modeling of the mitochondria-ROS generating pathway based on experiment and systemic analysis using the model have not been attempted so far. Thus, we conducted experiments to measure the concentration changes of critical molecules associated with mitochondrial apoptosis in both human mesothelioma H2052 and their ρ0cells lacking mitochondrial DNA (mtDNA). Based on the experiments,... -
Computer-Aided Drug Discovery in Plant Pathology
한국식물병리학회 The Plant Pathology Journal 14 Pages
한국식물병리학회 The Plant Pathology Journal 2017, 33권 6호 1 529-542 (14 pages)
approaches that are being employed in modern drug discovery. In order to help readers to dive into CADD, we explain concepts of homology modelling, molecular docking, virtual screening, and de novo ligand design in structure-based CADD, and pharmacophore modelling, ligand-based virtual screening, quantitative structure activity relationship modelling and de novo ligand design for ligand-based CADD. We also provide the important resources available to carry out CADD. Finally, we present a case... -
Hierarchical porous carbon nanofibers via electrospinning
Aikifa Raza, Jiaqi Wang, Shan Yang, Yang Si, Bin Ding 한국탄소학회 Carbon Letters 14 Pages
한국탄소학회 Carbon Letters 2014, Vol.15 No.1 1 1-14 (14 pages)
applications accomplished by appropriately regulating the microstructural, mechanical, and electrical properties of as-spun CNFs. Additionally, the article describes the various strategies to make a variety of carbon CNFs for energy conversion and storage, catalysis, sensor, adsorption/separation, and biomedical applications. It is envisioned that electrospun CNFs will be the key materials of green science and technology through close collaborations with carbon fibers and carbon nanotubes. -
요구도 기반 항공우주 시스템 강건최적설계 기법 연구
박형욱, 이재우, 변영환, 정준, Park. Hyeong-Uk, Lee. Jae-Woo, Byun. Yung-Hwan, Chung. Joon 한국항공우주학회 韓國航空宇宙學會誌 12 Pages
한국항공우주학회 韓國航空宇宙學會誌 2009, Vol.37 No.3 255-266 (12 pages)
본 연구에서는 기존 설계요구도 정립과정에서 고려할 수 없었던 설계자의 주관적인 판단의 배제 및 사용자 요구도의 변화, 시스템의 생산 및 운용환경에서 발생하는 오차 등을 개념 설계과정에서 고려하는 설계 프로세스를 개발하였다. 사용자 요구도를 의사결정 모델 및 시스템 엔지니어링 기법을 사용하여 구체화하여 대안형상들을 도출하고 그 가운데에서 우수한 대안형상을 선정한다. 선정된 대안형상들에 대해 최적설계 기법을 적용한 개념설계 결과를 도출하고 여기에 강건최적설계 기법을 적용하여 사용자의 요구를 만족하면서... -
Detection of Clavibacter michiganensis subsp. michiganensis Assisted by Micro- Raman Spectroscopy under Laboratory Conditions
Moisé, s Roberto Vallejo Pé, rez, Hugo Ricardo Navarro Contreras, Jesú, s A. Sosa Herrera, José, Pablo Lara Á, vila 한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2018, 34권 5호 4 381-392 (12 pages)
The implementation and validation of new technologies is necessary to reduce the time for bacterial detection in laboratory conditions and Raman spectroscopy is an ambitious technology that has all of the features needed to characterize and identify bacteria. Under controlled conditions a contagion process was induced with Cmm, the disease epidemiology was monitored. Micro-Raman spectroscopy (532 nm λ laser) technique was evaluated its performance at assisting on Cmm detection through its...
