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21.

Note : Open Access ; Occurrence of dsRNA Mycovirus (LeV-FMRI0339) in the Edible Mushroom Lentinula edodes and Meiotic Stability of LeV-FMRI0339 among Monokaryotic Progeny

한국식물병리학회 The Plant Pathology Journal 5 Pages

한국식물병리학회 The Plant Pathology Journal 2013, 29권 4호 14 460-464 (5 pages)

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- 1,500원
dsRNA was found in malformed cultures of Lentinula edodes strain FMRI0339, one of the three most popular sawdust cultivated commercial strains of shiitake, and was also found in healthy-looking fruiting bodies and actively growing mycelia. Cloning of the partial genome of the dsRNA revealed the presence of the RdRp sequence of a novel L. edodes mycovirus (LeV), and sequence comparison of the cloned amplicon showed identical sequences sequence to known RNA-dependent RNA polymerase genes of LeV...
22.

Note : Open Access ; Development of a Selective Medium for the Fungal Pathogen Fusarium graminearum Using Toxoflavin Produced by the Bacterial Pathogen Burkholderia glumae

한국식물병리학회 The Plant Pathology Journal 5 Pages

한국식물병리학회 The Plant Pathology Journal 2013, 29권 4호 11 446-450 (5 pages)

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- 1,500원
The ascomycete fungus Fusarium graminearum is a major causal agent for Fusarium head blight in cereals and produces mycotoxins such as trichothecenes and zearalenone. Isolation of the fungal strains from air or cereals can be hampered by various other airborne fungal pathogens and saprophytic fungi. In this study, we developed a selective medium specific to F. graminearum using toxoflavin produced by the bacterial pathogen Burkholderia glumae. F. graminearum was resistant to toxoflavin, while...
23.

Open Access ： Enhanced Biological Control of Phytophthora Blight of Pepper by Biosurfactant-Producing Pseudomonas

한국식물병리학회 The Plant Pathology Journal 9 Pages

한국식물병리학회 The Plant Pathology Journal 2013, 29권 4호 7 418-426 (9 pages)

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- 3,000원
Pseudomonas isolates from different crop plants were screened for in vitro growth inhibition of Phytophthora capsici and production of biosurfactant. Two in vivo experiments were performed to determine the efficacy of selected Pseudomonas strains against Phytophthora blight of pepper by comparing two fungicide treatments [acibenzolar-S-methyl (ASM) and ASM + mefenoxam]. Bacterial isolates were applied by soil drenching (1 × 109 cells/ml), ASM (0.1 μg a.i./ml) and ASM + mefenoxam...
24.

Open Access : Biological and Molecular Variability of Alfalfa mosaic virus Affecting Alfalfa Crop in Riyadh Region

한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2013, 29권 4호 6 410-417 (8 pages)

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- 3,000원
In 2011-2012, sixty nine samples were collected from alfalfa plants showing viral infection symptoms in Riyadh region. Mechanical inoculation with sap prepared from two collected samples out of twenty five possitive for Alfalfa mosaic virus (AMV) by ELISA were produced systemic mosaic on Vigna unguiculata and Nicotiana tabacum, local lesion on Chenopodium amaranticolor and C. quinoa. Vicia faba indicator plants that induce mosaic and mottle with AMV-Sagir isolate and no infection with AMV-Wadi...
25.

Open Access : Molecular Characterization and Variation of the Broad bean wilt virus 2 Isolates Based on Analyses of Complete Genome Sequences

한국식물병리학회 The Plant Pathology Journal 13 Pages

한국식물병리학회 The Plant Pathology Journal 2013, 29권 4호 5 397-409 (13 pages)

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- 4,200원
The full-genome sequences of fourteen isolates of Broad bean wilt virus 2 (BBWV2), collected from broad bean, pea, spinach, bell pepper and paprika plants in Korea during the years 2006-2012, were determined and analyzed comparatively along with fifteen previously reported BBWV2 genome sequences. Sequence analyses showed that RNA-1 and RNA-2 sequences of BBWV2 Korean isolates consisted of 5950-5956 and 3568-3604 nucleotides, respectively. Full-length genome sequencebased phylogenetic analyses...
26.

Open Access : Hydrogen Peroxide- and Nitric Oxide-mediated Disease Control of Bacterial Wilt in Tomato Plants

한국식물병리학회 The Plant Pathology Journal 11 Pages

한국식물병리학회 The Plant Pathology Journal 2013, 29권 4호 4 386-396 (11 pages)

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- 4,000원
Reactive oxygen species (ROS) generation in tomato plants by Ralstonia solanacearum infection and the role of hydrogen peroxide (H2O2) and nitric oxide in tomato bacterial wilt control were demonstrated. During disease development of tomato bacterial wilt, accumulation of superoxide anion (O2 -) and H2O2 was observed and lipid peroxidation also occurred in the tomato leaf tissues. High doses of H2O2 and sodium nitroprusside (SNP) nitric oxide donor showed phytotoxicity to detached tomato leaves...
27.

Open Access : Altered Gene Expression and Intracellular Changes of the Viable But Nonculturable State in Ralstonia solanacearum by Copper Treatment

한국식물병리학회 The Plant Pathology Journal 12 Pages

한국식물병리학회 The Plant Pathology Journal 2013, 29권 4호 3 374-385 (12 pages)

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- 4,100원
Environmental stresses induce several plant pathogenic bacteria into a viable but nonculturable (VBNC) state, but the basis for VBNC is largely uncharacterized. We investigated the physiology and morphology of the copperinduced VBNC state in the plant pathogen Ralstonia solanacearum in liquid microcosm. Supplementation of 200 μM copper sulfate to the liquid microcosm completely suppressed bacterial colony formation on culture media; however, LIVE/DEAD BacLight bacterial viability staining...
28.

Open Access : Xanthomonas oryzae pv. oryzae RpfE Regulates Virulence and Carbon Source Utilization without Change of the DSF Production

한국식물병리학회 The Plant Pathology Journal 10 Pages

한국식물병리학회 The Plant Pathology Journal 2013, 29권 4호 2 364-373 (10 pages)

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- 3,000원
It has been known that most regulation of pathogenicity factor (rpf ) genes in xanthomonads regulates virulence in response to the diffusible signal factor, DSF. Although many rpf genes have been functionally characterized, the function of rpfE is still unknown. We cloned the rpfE gene from a Xanthomonas oryzae pv. oryzae (Xoo) Korean race KACC10859 and generated mutant strains to elucidate the role of RpfE with respect to the rpf system. Through experiments using the rpfE-deficient mutant...
29.

Open Access : Specific and Sensitive Detection of Venturia nashicola, the Scab Fungus of Asian Pears, by Nested PCR

한국식물병리학회 The Plant Pathology Journal 7 Pages

한국식물병리학회 The Plant Pathology Journal 2013, 29권 4호 1 357-363 (7 pages)

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- 3,000원
The fungus Venturia nashicola is the causal agent of scab on Asian pears. For the rapid and reliable identification as well as sensitive detection of V. nashicola, a PCR-based technique was developed. DNA fingerprints of three closely related species, V. nashicola, V. pirina, and V. inaequalis, were obtained by random amplified polymorphic DNA (RAPD) analysis. Two RAPD markers specific to V. nashicola were identified by PCR, after which two pairs of sequence characterized amplified region (SCAR)...
30.

Genetic Reassortment of Rice stripe virus RNA Segments Detected by RT-PCR Restriction Enzyme Analysis-based Method

한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2011, 27권 2호 7 148-155 (8 pages)

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- 3,000원
reverse transcription- polymerase reaction/restriction enzyme (RTPCR/ RE) analysis-based method. We identified five REs, including DraI, EcoR1, NdeI/AseI, and SpeI, that could differentiate RSV RNA 1-4 subtypes, respectively. Our RT-PCR/RE results provided a clear pattern of RNA reassortment, i.e., different groups of isolates having their RNA segments derived from two to three different RSV ancestors, such as from Eastern and Southwestern Chinese or Japanese M and T isolates. We also found that...

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