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21.

Rapid and Visual Detection of Barley Yellow Dwarf Virus by Reverse Transcription Recombinase Polymerase Amplification with Lateral Flow Strips

Na-Kyeong Kim, Hyo-Jeong Lee, Sang-Min Kim, Rae-Dong Jeong 한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2022, 38권 2호 8 159-166 (8 pages)

Na-Kyeong Kim, Hyo-Jeong Lee, Sang-Min Kim, Rae-Dong Jeong 저
...
- 3,000원
rapid and reliable method for isothermal reverse transcription recombinase polymerase amplification (RT-RPA) combined with a lateral flow strips (LFS) assay for the detection of BYDV-infected oat samples based on the conserved sequences of the BYDV coat protein gene. Specific primers and a probe for RT-RPA reacted and optimally incubated at 42oC for 10 min, and the end-labeled amplification products were visualized on LFS within 10 min. The RT-RPA-LFS assay showed no cross-reactivity with other...
22.

Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid and Sensitive Detection of Barley Yellow Dwarf Virus in Oat

Na-Kyeong Kim, Sang-Min Kim, Rae-Dong Jeong 한국식물병리학회 The Plant Pathology Journal 6 Pages

한국식물병리학회 The Plant Pathology Journal 2020, 36권 5호 12 497-502 (6 pages)

Na-Kyeong Kim, Sang-Min Kim, Rae-Dong Jeong 저
...
- 3,000원
(BYDV) is an economically important plant pathogen that causes stunted growth, delayed heading, leaf yellowing, and purple leaf tip, thereby reducing the yields of cereal crops worldwide. In the present study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay was developed for the detection of BYDV in oat leaf samples. The RT-RPA assay involved incubation at an isothermal temperature (42°C) and could be performed rapidly in 5 min. In addition, no cross-reactivity was...
23.

Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification

Nam-Yeon Kim, Jong-hee Oh, Su-Heon Lee, Hong-sup Kim, Jae Sun Moon, Rae-Dong Jeong 한국식물병리학회 The Plant Pathology Journal 5 Pages

한국식물병리학회 The Plant Pathology Journal 2018, 34권 6호 12 575-579 (5 pages)

Nam-Yeon Kim, Jong-hee Oh, Su-Heon Lee, Hong-sup Kim, Jae Sun Moon, Rae-Dong Jeong 저
...
- 1,500원
a novel assay for specific ASGV detection that is based on reverse transcription-recombinase polymerase amplification is described. This assay has been shown to be reproducible and able to detect as little as 4.7 ng/μl of purified RNA obtained from an ASGV-infected plant. The major advantage of this assay is that the reaction for the target virus is completed in 1 min, and amplification only requires an incubation temperature of 42°C. This assay is a promising alternative method for pear...
24.

A New Distinct Clade for Iranian Tomato spotted wilt virus Isolates Based on the Polymerase, Nucleocapsid, and Non-structural Genes

Mahsa Abadkhah, Davoud Koolivand, Omid Eini 한국식물병리학회 The Plant Pathology Journal 18 Pages

한국식물병리학회 The Plant Pathology Journal 2018, 34권 6호 7 514-531 (18 pages)

Mahsa Abadkhah, Davoud Koolivand, Omid Eini 저
...
- 4,700원
to the RNA polymerase gene and DNA fragments of ca 777 bp and 724 bp in length were amplified using specific primers that have been designed based on the nucleocapsid (N) and non-structural (NSs) genes, respectively. The amplified fragments were cloned into pTG19-T and sequenced. Sequence comparisons with those available in the GenBank showed that the sequences belong to TSWV. The high nucleotide identity and similarities of new sequences based on the L, N, and NSs genes showed that minor...
25.

Simultaneous Detection of Three Bacterial Seed-Borne Diseases in Rice Using Multiplex Polymerase Chain Reaction

한국식물병리학회 The Plant Pathology Journal 5 Pages

한국식물병리학회 The Plant Pathology Journal 2016, 32권 6호 11 575-579 (5 pages)

저
...
- 1,500원
Burkholderia glumae (bacterial grain rot), Xanthomonas oryzae pv. oryzae (bacterial leaf blight), and Acidovorax avenae subsp. avenae (bacterial brown stripe) are major seedborne pathogens of rice. Based on the 16S and 23S rDNA sequences for A. avenae subsp. avenae and B. glumae, and transposase A gene sequence for X. oryzae pv. oryzae, three sets of primers had been designed to produce 402 bp for B. glumae, 490 bp for X. oryzae, and 290 bp for A. avenae subsp. avenae with the 63°C as an...
26.

Research Notes : Reverse Transcription Polymerase Chain Reaction-based System for Simultaneous Detection of Multiple Lily-infecting Viruses

한국식물병리학회 The Plant Pathology Journal 6 Pages

한국식물병리학회 The Plant Pathology Journal 2013, 29권 3호 13 338-343 (6 pages)

저
...
- 3,000원
multiplex reverse transcription (RT) polymerase chain reaction (PCR) was developed to simultaneously identify multiple viruses in the lily plant. The most common viruses infecting lily plants are the cucumber mosaic virus (CMV), lily mottle virus (LMoV), lily symptomless virus (LSV). Leaf samples were collected at lily-cultivation facilities located in the Kangwon province of Korea and used to evaluate the detection system. Simplex and multiplex RT-PCR were performed using virus-specific primers...
27.

벼 흰잎마름병의 신속하고 간편한 진단을 위한 Recombinase Polymerase Amplification 등온증폭법

김신화, 이봉춘, 김현주, 최수연, 서수좌, 김상민 한국식물병리학회 식물병연구 7 Pages

한국식물병리학회 식물병연구 2020, 26권 4호 1 195-201 (7 pages)

김신화, 이봉춘, 김현주, 최수연, 서수좌, 김상민 저
...
- 3,000원
중 하나인 recombinase polymerase amplification (RPA)를 적용하여 현장진단 등을 위한 유전자기반 진단법을 개발하였다. RPA법은 짧은 시간의 등온 조건에서 유전자 증폭이 가능하다는 장점이 있다. 본 연구에서개발한 벼 흰잎마름병 RPA 진단법은 39oC에서 5분간만 반응하면 목표유전자의 증폭이 이루어지므로 기존 진단법보다 신속하고 간편하며 우리나라에 존재하는 K1–K3a의 4종 레이스에모두 적용가능하며, DNA 추출 없이 식물체 잎의 즙액으로 증폭반응 수행이 가능하며 기존 Taq 기반 PCR보다 약 10배 검출민감도가 높고...
28.

Development of a Multiplex Reverse Transcription-Polymerase Chain Reaction Assay for the Simultaneous Detection of Three Viruses in Leguminous Plants

Chung Youl Park, Hyun-Geun Min, Hong-Kyu Lee, Rameswor Maharjan, Youngnam Yoon, Su-Heon Lee 한국식물병리학회 식물병연구 5 Pages

한국식물병리학회 식물병연구 2018, 24권 4호 15 348-352 (5 pages)

Chung Youl Park, Hyun-Geun Min, Hong-Kyu Lee, Rameswor Maharjan, Youngnam Yoon, Su-Heon Lee 저
...
- 1,500원
multiplex reverse transcription-polymerase chain reaction (mRT-PCR) assay was developed for the detection of Clover yellow vein virus (ClYVV), Peanut mottle virus (PeMoV), and Tomato spotted wilt virus (TSWV), which were recently reported to infect soybean and azuki bean in Korea. Species-specific primer sets were designed for the detection of each virus, and their specificity and sensitivity were tested using mixed primer sets. From among the designed primer sets, two combinations were selected...
29.

미생물,면역학 : 오제스키병 바이러스 검출을 위한 Polymerase Chain Reaction

황동희, 여상건 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 8 Pages

대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2003, 제 43권 제 2호 9 239-246 (8 pages)

황동희, 여상건 저
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- 3,000원
30.

미생물,면역학 : 장구균의 vancomycin 내성 유전자와 종 특이유전자의 검출을 위한 Multiplex polymerase chain reaction 개발

조윤상, 이희수, 김종만, 안종삼, 류판동, 박용호 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 10 Pages

대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2003, 제 43권 제 1호 13 103-112 (10 pages)

조윤상, 이희수, 김종만, 안종삼, 류판동, 박용호 저
...
- 3,000원

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