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Evaluation of modified vaccinia virus Ankara expressing VP2 protein of infectious bursal disease virus as an immunogen in chickens
Flavia Adriana Zanetti*, Mar?a Paula Del M?dico Zajac, Oscar Alberto Taboga, Gabriela Calamante 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 12 199-201 (3 pages)
A recombinant modified vaccinia Ankara (MVA) virus expressing mature viral protein 2 (VP2) of the infectious bursal disease virus (IBDV) was constructed to develop MVA-based vaccines for poultry. We demonstrated that this recombinant virus was able to induce a specific immune response by observing the production of anti-IBDV-seroneutralizing antibodies in specific pathogen-free chickens. Besides, as the epitopes of VP2 responsible to induce IBDV-neutralizing antibodies are discontinuous, our... -
Pathotyping avian pathogenic Escherichia coli strains in Korea
Yong-Wun Jeong, Tae-Eun Kim, Jae-Hong Kim*, Hyuk-Joon Kwon* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 5 145-152 (8 pages)
To examine the genetic background of avian pathogenic Escherichia coli (APEC) that affects virulence of this microorganism, we characterized the virulence genes of 101 APEC strains isolated from infected chickens between 1985∼2005. Serotypes were determined with available anti-sera and median lethal doses were determined in subcutaneously inoculated chicks. The virulence genes we tested included ones encoding type 1 fimbriae (fimC), iron uptake-related (iroN, irp2, iucD, and fyuA), toxins (lt,... -
Molecular characterization of Escherichia coli O157:H7 strains isolated from different sources and geographic regions
Adriana Hamond Regua-Mangia*, Alice Gon?alves M. Gonzalez, Aloysio M. F. Cerqueira, Joao Ramos C. Andrade 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 4 139-144 (6 pages)
Escherichia (E.) coli serotype O157:H7 is a globally distributed human enteropathogen and is comprised of microorganisms with closely related genotypes. The main reservoir for this group is bovine bowels, and infection mainly occurs after ingestion of contaminated water and food. Virulence genetic markers of 28 O157:H7 strains were investigated and multilocus enzyme electrophoresis (MLEE) was used to evaluate the clonal structure. O157:H7 strains from several countries were isolated from food,... -
Sequence variations of the bovine prion protein gene (PRNP) in native Korean Hanwoo cattle
Sangho Choi, Hee-Jong Woo*, Jeongmin Lee* 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 3 127-137 (11 pages)
Bovine spongiform encephalopathy (BSE) is one of the fatal neurodegenerative diseases known as transmissible spongiform encephalopathies (TSEs) caused by infectious prion proteins. Genetic variations correlated with susceptibility or resistance to TSE in humans and sheep have not been reported for bovine strains including those from Holstein, Jersey, and Japanese Black cattle. Here, we investigated bovine prion protein gene (PRNP) variations in Hanwoo cattle [Bos (B.) taurus coreanae], a native... -
Establishment and characterization of an infectious cDNA clone of a classical swine fever virus LOM strain
Gil-Soon Park, Seong-In Lim, Seung-Ho Hong, Jae-Young Song* 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 12 81-91 (11 pages)
PK-15 cells. The rescued Flc-LOM virus grew more slowly than the parental virus, LOM, in the cells. Intramuscular immunization with Flc-LOM was safe and highly immunogenic in pigs; no clinical signs or virus transmission to sentinel animals were observed after 35 days. CSFV-specific neutralizing antibodies were detected 14 days post-infection. After challenge with the virulent CSFV strain SW03, pigs immunized with Flc-LOM were shown to be fully protected. Thus, our newly established infectious... -
Development and characterization of a potential diagnostic monoclonal antibody against capsid protein VP1 of the chicken anemia virus
Yi-Yang Lien, Chi-Hung Huang, Fang-Chun Sun, Shyang-Chwen Sheu, Tsung-Chi Lu, Meng-Shiunn Lee, Shu-Chin Hsueh, Hsi-Jien Chen, Me 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 11 73-79 (7 pages)
Chicken anemia virus (CAV) is an important viral pathogen that causes anemia and severe immunodeficiency syndrome in chickens worldwide. In this study, a potential diagnostic monoclonal antibody against the CAV VP1 protein was developed which can precisely recognize the CAV antigen for diagnostic and virus recovery purposes. The VP1 gene of CAV encoding the N-terminus-deleted VP1 protein, VP1Nd129, was cloned into an Escherichia (E.) coli expression vector. After... -
Cloning and characterization of a selenium-independent glutathione peroxidase (HC29) from adult Haemonchus contortus
Wei Sun, Xiaokai Song, Ruofeng Yan, Lixin Xu, Xiangrui Li* 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 8 49-58 (10 pages)
The complete coding sequence of Haemonchus (H.) contortus HC29 cDNA was generated by rapid amplification of cDNA ends in combination with PCR using primers targeting the 5´- and 3´-ends of the partial mRNA sequence. The cloned HC29 cDNA was shown to be 1,113 bp in size with an open reading frame of 507 bp, encoding a protein of 168 amino acid with a calculated molecular mass of 18.9 kDa. Amino acid sequence analysis revealed that the cloned HC29 cDNA contained the conserved catalytic triad and... -
Generation of transgenic corn-derived Actinobacillus pleuropneumoniae ApxIIA fused with the cholera toxin B subunit as a vaccine candidate
Min-Kyoung Shin, Myung Hwan Jung, Won-Jung Lee, Pil Son Choi, Yong-Suk Jang, Han Sang Yoo* 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 14 401-403 (3 pages)
Corn, one of the most important forage crops worldwide, has proven to be a useful expression vehicle due to the availability of established transformation procedures for this well-studied plant. The exotoxin Apx, a major virulence factor, is recognized as a common antigen of Actinobacillus (A.) pleuropneumoniae, the causative agent of porcine pleuropneumonia. In this study, a cholera toxin B (CTB)-ApxIIA#5 fusion protein and full-size ApxIIA expressed in corn seed, as a subunit vaccine... -
Molecular characterization of a 13-amino acid deletion in VP1 (1D) protein and novel amino acid substitutions in 3D polymerase protein of foot and mouth disease virus subtype A/Iran87
Majid Esmaelizad*, Saber Jelokhani-Niaraki, Khadije Hashemnejad, Morteza Kamalzadeh, Mohsen Lotfi 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 9 363-371 (9 pages)
The nucleotide sequence of the VP1 (1D) and partial 3D polymerase (3D) coding regions of the foot and mouth disease virus (FMDV) vaccine strain A/Iran87, a highly passaged isolate (∼150 passages), was determined and aligned with previously published FMDV serotype A sequences. Overall analysis of the amino acid substitutions revealed that the partial 3D coding region contained four amino acid alterations. Amino acid sequence comparison of the VP1 coding region of the field isolates revealed... -
Apoptosis induced in vivo by new type gosling viral enteritis virus
Shun Chen, Anchun Cheng*, Mingshu Wang*, Dekang Zhu, Renyong Jia, Qihui Luo, Hengmin Cui, Yi Zhou, Yin Wang, Zhiwen Xu, Zhengli 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 5 333-339 (7 pages)
extracted from the NGVEV- infected gosling cells displayed characteristic 180∼200 bp ladders. Apoptotic cells were ubiquitously distributed, especially among lymphocytes, macrophages, monocytes, and epithelial and intestinal cells. Necrosis was subsequently detected during the late NGVEV-infection phase, which was characterized by cell swelling, plasma membrane collapse, and rapidly lysis. Our results suggested that apoptosis may play an important role in the pathogenesis of NGVE disease.
