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291.

Occurrence and characterization of livestock-associated methicillin-resistant Staphylococcus aureus in pig industries of northern Thailand

Prapas Patchanee, , Pakpoom Tadee, Orapun Arjkumpa, David Love, Karoon Chanachai, Thomas Alter, Soawapak Hinjoy, Prasit Tharavich 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 10 529-536 (8 pages)

Prapas Patchanee, , Pakpoom Tadee, Orapun Arjkumpa, David Love, Karoon Chanachai, Thomas Alter, Soawapak Hinjoy, Prasit Tharavich 저
...
- 3,000원
This study was conducted to determine the prevalence of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) in pigs, farm workers, and the environment in northern Thailand, and to assess LA-MRSA isolate phenotypic characteristics. One hundred and four pig farms were randomly selected from the 21,152 in Chiang Mai and Lamphun provinces in 2012. Nasal and skin swab samples were collected from pigs and farm workers. Environmental swabs (pig stable floor, faucet, and feeder)...
292.

Enhancing effects of serum-rich and cytokine-supplemented culture conditions on developing blastocysts and deriving porcine parthenogenetic embryonic stem cells

Soo-Kyung Jung, Hyun-Jung Kim, Chan-Lan Kim, Joo-Hyeong Lee, Jin-Young You, Eun-Song Lee, Jeong-Mook Lim, Seon Jong Yun, Jae-You 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 9 519-528 (10 pages)

Soo-Kyung Jung, Hyun-Jung Kim, Chan-Lan Kim, Joo-Hyeong Lee, Jin-Young You, Eun-Song Lee, Jeong-Mook Lim, Seon Jong Yun, Jae-You 저
...
- 3,000원
The present study was conducted to develop an effective method for establishment of porcine parthenogenetic embryonic stem cells (ppESCs) from parthenogenetically activated oocyte-derived blastocysts. The addition of 10% fetal bovine serum (FBS) to the medium on the 3rd day of oocyte culturing improved the development of blastocysts, attachment of inner cell masses (ICMs) onto feeder cells, and formation of primitive ppESC colonies. ICM attachment was further enhanced by basic fibroblast growth...
293.

Feasibility of utilizing the patellar ligament angle for assessing cranial cruciate ligament rupture in dogs

Jung-ha Lee, Soon-wuk Jeong 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 8 563-568 (6 pages)

Jung-ha Lee, Soon-wuk Jeong 저
...
- 3,000원
cranial cruciate ligament (CrCL). The PLA of stifles with complete CrCL rupture was significantly lower than that of normal stifles, particularly at a flexion angle of 60∼80o in both plain and stress views. If the PLA was ＜90.55o on the stress view with a 60∼80o flexion angle, the dog was diagnosed with a complete rupture of the CrCL with a sensitivity of 83.9% and specificity of 100%. In conclusion, measuring the PLA is a quantitative method for diagnosing complete CrCL rupture in canines.
294.

Use of hydrophilic extra-viral domain of canine distemper virus H protein for enzyme-linked immunosorbent assay development

Ki-hyun Cho, Jeongmi Kim, Hyun-ah Yoo, Dae-hee Kim, Seung-yong Park, Chang-seon Song, In-soo Choi, Joong-bok Lee, 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 6 503-509 (7 pages)

Ki-hyun Cho, Jeongmi Kim, Hyun-ah Yoo, Dae-hee Kim, Seung-yong Park, Chang-seon Song, In-soo Choi, Joong-bok Lee, 저
...
- 3,000원
Simple methods for measuring the levels of serum antibody against canine distemper virus (CDV) would assist in the effective vaccination of dogs. To develop an enzyme-linked immunosorbent assay (ELISA) specific for CDV, we expressed hydrophilic extra-viral domain (HEVD) protein of the A75/17-CDV H gene in a pET 28a plasmid-based Escherichia (E.) coli vector system. Expression was confirmed by dot and Western blotting. We proposed that detection of E. coli-expressed H protein might be...
295.

M gene analysis of canine coronavirus strains detected in Korea

Seok-Young Jeoung, So-Yun Ann, Hyun-Tae Kim, Doo Kim, 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 5 495-502 (8 pages)

Seok-Young Jeoung, So-Yun Ann, Hyun-Tae Kim, Doo Kim, 저
...
- 3,000원
The purpose of this study was to investigate the genetic features of canine coronavirus (CCV) strains detected in Korea. M gene sequences obtained for isolates from 22 dogs with enteritis over a 5-year period were evaluated. Sequence comparison revealed that the 22 Korean CCV strains had an 87.2 to 100% nucleotide homology. Comparing to the typical reference CCV strains (type II), the nucleotide sequence of Korean strains had homology ranged from 86.3% to 98.3% (89.1% to 99.2% for the amino acid...
296.

N-acetylcysteine protects against cadmium-induced oxidative stress in rat hepatocytes

Jicang Wang, Huali Zhu, Xuezhong Liu, Zongping Liu, 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 4 485-493 (9 pages)

Jicang Wang, Huali Zhu, Xuezhong Liu, Zongping Liu, 저
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- 3,000원
Cadmium (Cd) is a well-known hepatotoxic environmental pollutant. We used rat hepatocytes as a model to study oxidative damage induced by Cd, effects on the antioxidant systems, and the role of N-acetylcysteine (NAC) in protecting cells against Cd toxicity. Hepatocytes were incubated for 12 and 24 h with Cd (2.5,  5, 10 μM). Results showed that Cd can induce cytotoxicity: 10 μM resulted in 36.2% mortality after 12 h and 47.8% after 24 h. Lactate dehydrogenase, aspartate...
297.

Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage

Li-ming Wu, Rui Guo, Lin Hui, Yong-gang Ye, Jing-mei Xiang, Chun-yun Wan, Miao Zou, Rui Ma, Xiao-zhuan Sun, Shi-jin Yang, Ding-z 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 3 475-483 (9 pages)

Li-ming Wu, Rui Guo, Lin Hui, Yong-gang Ye, Jing-mei Xiang, Chun-yun Wan, Miao Zou, Rui Ma, Xiao-zhuan Sun, Shi-jin Yang, Ding-z 저
...
- 3,000원
Chronic enteritis can produce an excess of reactive oxygen species resulting in cellular damage. Stanniocalcin-1(STC-1) reportedly possesses anti-oxidative activity, the aim of this study was to define more clearly the direct contribution of STC-1 to anti-oxidative stress in cattle. In this study, primary intestinal epithelial cells (IECs) were exposed to hydrogen peroxide (H2O2) for different time intervals to mimic chronic enteritis-induced cellular damage. Prior to treatment with 200 μM...
298.

Developmental changes in cell proliferation and apoptosis in the normal duck bursa of Fabricius

Jing Fang, Xi Peng 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 2 465-474 (10 pages)

Jing Fang, Xi Peng 저
...
- 3,000원
The aim of this work was to investigate developmental changes in cell proliferation and apoptosis in normal duck bursa of Fabricius using flow cytometry and immunohistochemistry. Studies were carried out on Tianfu ducks on days 24 and 27 of embryogenesis (E24 and E27) along with days 20, 70, and 200 of postnatal development (P20, P70, and P200). Results showed that the percentage of G0/G1 bursa cells significantly increased between E24 and P200 while the percentage of cells in the S phase or G2...
299.

Dietary germanium biotite supplementation enhances the induction of antibody responses to foot-and-mouth disease virus vaccine in pigs

Jin-A Lee, Bock-Gie Jung, Myunghwan Jung, Tae-Hoon Kim, Han Sang Yoo, Bong-Joo Lee* 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 15 443-447 (5 pages)

Jin-A Lee, Bock-Gie Jung, Myunghwan Jung, Tae-Hoon Kim, Han Sang Yoo, Bong-Joo Lee* 저
...
- 1,500원
We evaluated the potential ability of germanium biotite (GB) to stimulate the production of antibodies specific for foot-and-mouth disease virus (FMDV). To this aim, we measured the total FMDV-specific antibody responses and IgM production after vaccination against FMD both experimentally and in the field. GB supplementation with FMDV vaccination stimulated the production of anti-FMDV antibodies, and effectively increased IFN-γ and TNF-α levels. These results suggest that GB may be a novel...
300.

Quantitative measurement of influenza virus replication using consecutive bronchoalveolar lavage in the lower respiratory tract of a ferret model

Dong-Hun Lee, Jong-In Kim, Jae-Won Lee, Wook-Hun Chung, Jae-Keun Park, Yu-Na Lee, Jin Soo Han, Hwi-Yool Kim, Sang-Won Lee, Chang 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 14 439-442 (4 pages)

Dong-Hun Lee, Jong-In Kim, Jae-Won Lee, Wook-Hun Chung, Jae-Keun Park, Yu-Na Lee, Jin Soo Han, Hwi-Yool Kim, Sang-Won Lee, Chang 저
...
- 1,500원
nasal washes, daily evaluation of viral replication in the lung is limited because a large numbers of ferrets need to be sacrificed at consecutive time points. To overcome this limitation, we performed a virus quantification assay using bronchoalveolar lavage (BAL) fluid. This non-invasive BAL technique allows consecutive quantification of virus replication in the lungs of living ferrets. Our method can be used for the longitudinal evaluation of virus tropism in the lower respiratory tract.

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