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Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury
Hak-Hyun Ryu, Ji-Hey Lim, Ye-Eun Byeon, Jeong-Ran Park, Min-Soo Seo, Young-Won Lee, Wan Hee Kim, Kyung-Sun Kang*, Oh-Kyeong Kweo 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 4호 1 273-284 (12 pages)
In this study, we evaluated if the implantation of allogenic adipose-derived stem cells (ASCs) improved neurological function in a canine spinal cord injury model. Eleven adult dogs were assigned to three groups according to treatment after spinal cord injury by epidural balloon compression: C group (no ASCs treatment as control), V group (vehicle treatment with PBS), and ASC group (ASCs treatment). ASCs or vehicle were injected directly into the injured site 1 week after spinal cord injury.... -
Charting the proteome of Cryptosporidium parvum sporozoites using sequence similarity-based BLAST searching
A.M.A.M.Z. Siddiki*, Jonathan M. Wastling 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 5 203-210 (8 pages)
Cryptosporidium (C.) spp. are important zoonotic parasites causing widespread diarrhoeal disease in man and animals. The recent release of the complete genome sequences for C. parvum and C. hominis has facilitated the comprehensive global proteome analysis of these opportunistic pathogens. The well-known approach for mass spectrometry (MS) based data analysis using the BLAST tool (MS BLAST) is a database search protocol for identifying unknown proteins by sequence similarity to homologous... -
Post-thaw development of in vitro produced buffalo embryos cryopreserved by cytoskeletal stabilization and vitrification
B. M. Manjunatha*, J. P. Ravindra, P. S. P. Gupta, M. Devaraj, T. G. Honnappa, A. Krishnaswamy 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 2호 9 153-156 (4 pages)
0.3 M sucrose for 45 sec. After warming, the embryos were cultured in vitro for 72 h. The post-thaw in vitro developmental competence of the cyto-b-treated embryos did not differ significantly from those vitrified without cyto-b treatment. The hatching rates of morulae vitrified without cyto-b treatment was significantly lower than the non- vitrified control. However, the hatching rate of cyto-b- treated vitrified morulae did not differ significantly from the non-vitrified control. This study... -
Protection of chicken against very virulent IBDV provided by in ovo priming with DNA vaccine and boosting with killed vaccine and the adjuvant effects of plasmid-encoded chicken interleukin-2 and interferon-g
Jeong Ho Park, Haan Woo Sung, Byung Il Yoon, Hyuk Moo Kwon* 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 2호 6 131-140 (10 pages)
VP3 proteins of the very virulent IBDV (vvIBDV) SH/92 strain was injected into the amniotic sac alone or in combination with a plasmid encoding chicken IL-2 (ChIL-2) or chicken IFN-g (ChIFN-g) at embryonation day 18, followed by an intramuscular injection of a commercial killed IBD vaccine at 1 week of age. The chickens were orally challenged with the vvIBDV SH/92 strain at 3 weeks of age and observed for 10 days. In ovo DNA immunization followed by a killed- vaccine boost provided significantly... -
Genetic analysis of ORF5 of recent Korean porcine reproductive and respiratory syndrome viruses (PRRSVs) in viremic sera collected from MLV-vaccinating or non-vaccinating farms
Hye Kwon Kim, Jeong Sun Yang, Hyoung Joon Moon, Seong Jun Park, Yuzi Luo, Chul Seung Lee, Dae Sub Song, Bo Kyu Kang, Soo Kyung A 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 2호 5 121-130 (10 pages)
87.2∼88.9% similarity with the Lelystad virus, showing a close relationship with the 27∼2003 strain of Spain. The maximum parsimony tree of type II PRRSV from 1997 to 2008 showed that they had evolved to four lineages, subgroups 1, 2, 3 and 4. Most of the recently collected type II PRRSVs belonged to subgroup 4 (48%). The region of three B-cell epitopes and two T-cell epitopes of ORF5 amino acids sequences was considerably different from the MLV in subgroups 3 and 4. In conclusion,... -
Distribution and ontogeny of gastrin- and serotonin-immunoreactive cells in the proventriculus of developing chick, Gallus gallus domestica
Abdulkerim Aksoy*, Kenan Cinar 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 1호 2 9-13 (5 pages)
gastrin- and serotonin- immunoreactive (IR) cell in the proventriculus of chicks (Gallus gallus domestica, n = 60) in different growth periods was examined immunohistochemically using antisera specific to gastrin and serotonin. Gastrin and serotonin-IR cells were detected in chick proventriculus. Gastrin-IR cells were first evident after 12 days of incubation in lamina epithelialis and compound glands, while serotonin-IR cells were observed only in compound glands at that same time. Gastrin-IR... -
Implantation of canine umbilical cord blood-derived mesenchymal stem cells mixed with beta-tricalcium phosphate enhances osteogenesis in bone defect model dogs
Byung-Jun Jang, Ye-Eun Byeon, Ji-Hey Lim, Hak-Hyun Ryu, Wan Hee Kim, Yoshihisa Koyama, Masanori Kikuchi, Kyung-Sun Kang*, Oh-Kye 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 4호 8 387-394 (8 pages)
This study was performed to evaluate the osteogenic effect of allogenic canine umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) mixed with beta-tricalcium phosphate (b-TCP) in orthotopic implantation. Seven hundred milligrams of b-TCP mixed with 1 × 10 UCB-MSCs diluted with 0.5 ml of saline (group CM) and mixed with the same volume of saline as control (group C) were implanted into a 1.5 cm diaphyseal defect and wrapped with PLGC membrane in the radius of Beagle dogs.... -
In vitro and in vivo gene therapy with CMV vector-mediated presumed dog β-nerve growth factor in pyridoxine-induced neuropathy dogs
Jin-Young Chung, Jung-Hoon Choi, Il-Seob Shin, Eun-Wha Choi, Cheol-Yong Hwang, Sang-Koo Lee, Hwa-Young Youn* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 4호 5 367-374 (8 pages)
Due to the therapeutic potential of gene therapy for neuronal injury, many studies of neurotrophic factors, vectors, and animal models have been performed. The presumed dog β-nerve growth factor (pdβ-NGF) was generated and cloned and its expression was confirmed in CHO cells. The recombinant pdβ-NGF protein reacted with a human β-NGF antibody and showed bioactivity in PC12 cells. The pdβb-NGF was shown to have similar bioactivity to the dog β-NGF. The recombinant pdβ-NGF plasmid was... -
Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs
Ji-Hey Lim, Ye-Eun Byeon, Hak-Hyun Ryu, Yun-Hyeok Jeong, Young-Won Lee, Wan Hee Kim, Kyung-Sun Kang, Oh-Kyeong Kweon 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 3호 12 275-282 (8 pages)
4 and 8 week after transplantation. There was significant improvement in the nerve conduction velocity based on the somatosensory evoked potentials. In addition, a distinct structural consistency of the nerve cell bodies was noted in the lesion of the spinal cord of the UCB-MSC and UCBG groups. These results suggest that transplantation of the UCB-MSCs resulted in recovery of nerve function in dogs with a spinal cord injury and may be considered as a therapeutic modality for spinal cord injury. -
The micronucleus frequency in cytokinesis-blocked lymphocytes of cattle in the vicinity of a nuclear power plant
Hae-June Lee, Chang-Mo Kang, Se-Ra Kim, Jong-Choon Kim, Chun-Sik Bae, Ki-Seok Oh, Sung-Kee Jo, Tae-Hwan Kim, Jong-Sik Jang, Sung 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 3 117-120 (4 pages)
for the cattle from the Wolsong, Uljin and Yeonggwang nuclear power plants and for the control area were 9.87 ± 2.64, 8.90 ± 3.84, 9.20 ± 3.68 and 9.60 ± 3.91 per 1,000 cytokinesis-blocked lymphocytes, respectively. The apparent difference is not statistically significant. The MN frequencies of PBLs from cattle bred in the four areas are within the background variation for this study. The MN frequencies and hematological values were similar regardless of whether the cattle were bred near a...


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