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311.

Detection of porcine reproductive and respiratory syndrome virus in oral fluid from naturally infected pigs in a breeding herd

Nguyen Thi Trang, Takuya Hirai*, Tsukasa Yamamoto, Mari Matsuda, Naoko Okumura, Nguyen Thi Huong Giang, Nguyen Thi Lan, Ryoji Ya 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 4 361-367 (7 pages)

Nguyen Thi Trang, Takuya Hirai*, Tsukasa Yamamoto, Mari Matsuda, Naoko Okumura, Nguyen Thi Huong Giang, Nguyen Thi Lan, Ryoji Ya 저
...
- 3,000원
The objectives of the present study were to evaluate the anatomic localization of porcine reproductive and respiratory syndrome virus (PRRSV) in naturally infected pigs and to determine whether oral fluid could be used to detect the virus in infected animals. Two sows, seven 2-month-old grower pigs, and 70 6-month-old gilts were included in this study. PRRSV in sera and oral fluid were identified by nested reverse transcription PCR (nRT-PCR) while lung, tonsil, and tissue associated with oral...
312.

Distribution, quantitative load and characterization of Salmonella associated with swine farms in upper-northern Thailand

Pakpoom Tadee, Kittipong Kumpapong, Danai Sinthuya, Panuwat Yamsakul, Nipa Chokesajjawatee, Supachai Nuanualsuwan, Suchawan Porn 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 20 327-334 (8 pages)

Pakpoom Tadee, Kittipong Kumpapong, Danai Sinthuya, Panuwat Yamsakul, Nipa Chokesajjawatee, Supachai Nuanualsuwan, Suchawan Porn 저
...
- 3,000원
This study was conducted to analyze the prevalence and quantitative loads of Salmonella spp. on pig farms in Chiang Mai, Lamphun, Thailand to assess loading levels before slaughtering. The serotype diversity, antimicrobial-resistance pattern and pulse-field type of Salmonella spp. were also characterized to assess the dynamic propagation of the pathogen. The Salmonella-positive prevalence was 246/805 (30.56%), and the quantitative loads varied from 1.48∼4.04 Log10MPN/g, with a mean ± standard...
313.

Development of a multiplex loop-mediated isothermal amplification assay to detect shiga toxin-producing Escherichia coli in cattle

Hee-Jin Dong, Ae-Ri Cho, Tae-Wook Hahn, Seongbeom Cho* 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 19 317-325 (9 pages)

Hee-Jin Dong, Ae-Ri Cho, Tae-Wook Hahn, Seongbeom Cho* 저
...
- 3,000원
A multiplex loop-mediated isothermal amplification (mLAMP) assay was developed for simultaneous detection of the stx1 and stx2 genes and applied for detection of shiga toxin-producing Escherichia coli (STEC) in cattle farm samples. Two target genes were distinguished based on Tm values of 85.03 ± 0.54C for stx1 and 87.47 ± 0.35C for stx2. The mLAMP assay was specific (100% inclusivity and exclusivity), sensitive (with a detection limit as low as 10 fg/μL), and quantifiable (R = 0.9313). The...
314.

Accuracy of capillary blood 3-β-hydroxybutyrate determination for the detection and treatment of canine diabetic ketoacidosis

Francesca Bresciani, Marco Pietra, Sara Corradini, Massimo Giunti, Federico Fracassi* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 18 309-316 (8 pages)

Francesca Bresciani, Marco Pietra, Sara Corradini, Massimo Giunti, Federico Fracassi* 저
...
- 3,000원
In human medicine, diagnosis of diabetic ketoacidosis (DKA) is usually based on measurement of capillary 3-β-hydroxybutyrate (3-HB) with a hand held ketone sensor. This study was conducted to determine if measurement of capillary 3-HB could be useful for the diagnosis and monitoring of canine DKA. Fifteen dogs with diabetic ketosis and 10 with DKA were evaluated. Paired measurements of 3-HB of capillary and venous blood samples were analysed by the electrochemical sensor and reference method....
315.

Evaluation of endoscopically obtained duodenal biopsy samples from cats and dogs in an adapter-modified Ussing chamber

Isabelle Ruhnke*, John V. DeBiasio, Jan S. Suchodolski, Shelley Newman, Mark W. Musch, Jörg M. Steiner 대한수의학회 Journal of Veterinary Science 11 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 17 297-307 (11 pages)

Isabelle Ruhnke*, John V. DeBiasio, Jan S. Suchodolski, Shelley Newman, Mark W. Musch, Jörg M. Steiner 저
...
- 4,000원
from healthy cats and dogs, as well as dogs with chronic enteropathies. 17 duodenal biopsies from five cats and 51 duodenal biopsies from 13 dogs were obtained. Samples were transferred into an adapter-modified Ussing chamber and sequentially exposed to various absorbagogues and secretagogues. Overall, 78.6% of duodenal samples obtained from cats responded to at least one compound. In duodenal biopsies obtained from dogs, the rate of overall response ranged from 87.5% (healthy individuals; n =...
316.

Extent of Mycobacterium bovis infection in dairy cattle herds subject to partial culling as determined by an interferon-gamma assay

Sungmo Je, Un-chang Yeo, Taeksun Song, Ki-cheol Kim, Sung-Yun Park, Man-Jung Kim, Sang-Nae Cho* 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 12 259-265 (7 pages)

Sungmo Je, Un-chang Yeo, Taeksun Song, Ki-cheol Kim, Sung-Yun Park, Man-Jung Kim, Sang-Nae Cho* 저
...
- 3,000원
96-well plate format using the ESAT-6 and CFP-10 antigens and then employed it to determine the extent of Mycobacterium (M.) bovis infection in dairy herds with a history of BTB outbreaks in a country where only selective culling is practiced. The sensitivity and specificity of this IFN-γ assay were 85.9% and 100%, respectively, based on comparison with the conventional single intradermal tuberculin test (SIDT). The IFN-γ assay was also positive in 30.4% and 36.8% of SIDT-negative animals from...
317.

Equine hyperimmune serum protects mice against Clostridium difficile spore challenge

Weiwei Yan, Kang-Soon Shin, Shih-Jon Wang, Hua Xiang, Thomas Divers, Sean McDonough, James Bowman, Anne Rowlands, Bruce Akey, Hu 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 11 249-258 (10 pages)

Weiwei Yan, Kang-Soon Shin, Shih-Jon Wang, Hua Xiang, Thomas Divers, Sean McDonough, James Bowman, Anne Rowlands, Bruce Akey, Hu 저
...
- 3,000원
B as well as whole-cell bacteria. The toxin-neutralizing activities of the antibodies were evaluated in vitro and the prophylactic effects of in vivo passive immunotherapy were demonstrated using a conventional mouse model. The data demonstrated that immunized horses generated antibodies against both toxins A and B that possessed toxin-neutralizing activity. Additionally, mice treated with the antiserum lost less weight without any sign of illness and regained weight back to a normal range more...
318.

Isolation, in vitro propagation, genetic analysis, and immunogenic characterization of an Ehrlichia canis strain from southeastern Brazil

Rosiane Nascimento Alves*, Susana Elisa Rieck, Carlos Ueira-Vieira, Marcelo Bahia Labruna, Marcelo Emílio Beletti 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 10 241-248 (8 pages)

Rosiane Nascimento Alves*, Susana Elisa Rieck, Carlos Ueira-Vieira, Marcelo Bahia Labruna, Marcelo Emílio Beletti 저
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- 3,000원
Amplification of the 16S rRNA gene from a blood sample obtained from a dog in southeastern Brazil was used to confirm a naturally acquired Ehrlichia (E.) canis infection. Following isolation and culturing of the new bacterial strain called Uberlândia, partial sequences of the dsb and p28 genes were obtained. The dsb partial sequence of the novel strain was 100% similar to dsb gene sequences of E. canis obtained from different geographic areas around the world. Conversely, the p28...
319.

Recombinant Kluyveromyces lactis expressing highly pathogenic porcine reproductive and respiratory syndrome virus GP5 elicits mucosal and cell-mediated immune responses in mice

Haiyan Zhao, Yalan Wang, Zhitao Ma, Yongqiang Wang, Wen-hai Feng* 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 5 199-208 (10 pages)

Haiyan Zhao, Yalan Wang, Zhitao Ma, Yongqiang Wang, Wen-hai Feng* 저
...
- 3,000원
Currently, killed-virus and modified-live porcine reproductive and respiratory syndrome virus (PRRSV) vaccines are used to control porcine reproductive and respiratory syndrome. However, both types of vaccines have inherent drawbacks; accordingly, the development of novel PRRSV vaccines is urgently needed. Previous studies have suggested that yeast possesses adjuvant activities, and it has been used as an expression vehicle to elicit immune responses to foreign antigens. In this report,...
320.

Echocardiographic assessment of coronary artery flow in normal canines and model dogs with myocardial infarction

Nohwon Park, Jaehwan Kim, Miyoung Lee, Soyun Lee, Sunhye Song, Seungjun Lee, Soyoung Kim, Yangwoo Park, Kidong Eom* 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 17 149-155 (7 pages)

Nohwon Park, Jaehwan Kim, Miyoung Lee, Soyun Lee, Sunhye Song, Seungjun Lee, Soyoung Kim, Yangwoo Park, Kidong Eom* 저
...
- 3,000원
This study was conducted to evaluate the usefulness of coronary arterial profiles from normal dogs (11 animals) and canines (six dogs) with experimental myocardial infarction (MI) induced by ligation of the left coronary artery (LCA). Blood velocity of the LCA and right coronary artery (RCA) were evaluated following transthoracic pulsed-wave Doppler echocardiography. The LCA was observed as an infundibular shape, located adjacent to the sinus of Valsalva. The RCA appeared as a tubular structure...

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