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Reference map of soluble proteins from Salmonella enterica serovar Enteritidis by two-dimensional electrophoresis
Park MR, Lee EG, Kim YH, Jung TS, Shin YS, Shin GW, Cha HG, Kim GS 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 2호 7 143-149 (7 pages)
with a molecular mass ranging from 35-80 kDa. Some of these spots were identified as metabolic related enzymes. The protein fraction was also analyzed using an immobilized pH gradient strip. Different proteins were identified on the spot according to the elongation factors. In addition, this study showed that the 2-DE analysis of S. enteritidis provides useful information regarding the S. enteritidis proteome, and this approach might provide a strategy for identifying bacterial proteins using a... -
Research Articles : Distribution and Antifungal Activity of Endophytic Fungi in Different Growth Stages of Chili Pepper (Capsicum annuum L.) in Korea
한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2012, 28권 1호 2 10-19 (10 pages)
A total of 481 isolates were recovered and were identified using molecular techniques. Based on rDNA ITS gene sequence and phylogenetic analysis, 21 fungal genera were characterized, belonging to 16 Ascomycota and 5 Basidiomycota. Penicillium in seedling stage, Fusarium in flowering stage, Colletotrichum followed by Fusarium, Alternaria and Xylaria in fruiting stage was predominant and Alternaria, Cladosporium and Fusarium were common in all growth stages. Among 481 endophytes 90 phenotypes were... -
Effects of Recombination on the Pathogenicity and Evolution of Pepper mottle virus
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2009, 25권 4호 15 417-421 (5 pages)
The analysis of the full length genome of Korean isolates of Pepper mottle virus (PepMoV) in previous study showed molecular variations and are found to be related to symptom variation and pathogenicity (Kim et al., 2009, Virus Res. 144:83-88). To fully understand the molecular variation of PepMoV in Korea, we further assessed the role of RNA recombination to biological variation and evolution of PepMoV. Full-length genome of a total of 17 Korean-PepMoV and 2 American (CA and FL) isolates were... -
Generation of an Arginine Auxotrophic Mutant of Colletotrichum acutatum as a Recipient Host for Insertional Mutagenesis
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2009, 25권 3호 1 205-212 (8 pages)
facilitate molecular analysis of C. acutatum, we generated an arginine auxotrophic mutant of the C. acutatum strain JC24 using a targeted gene replacement strategy. A 3.3-kb genomic region carrying an ortholog (designated CaARG2) of the fungal gene encoding Nacetylglutamate synthase, the first enzyme of arginine biosynthesis in fungi, was deleted from the fungal genome. The mutant exhibited normal growth only when arginine was exogenously supplied into the culture medium. Transformation of the... -
Protoplast-Mediated Transformation of the Filamentous Fungus Cladosporium phlei: Evidence of Tandem Repeats of the Integrative Transforming Vector
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2009, 25권 2호 10 179-183 (5 pages)
from approximately 20-100 transformants per experiment. Southern blot analysis of stable transformants revealed that transformation occurred by way of stable integration of the vector DNA into the fungal chromosome. PCR analysis and plasmid rescuing of randomly selected transformants suggested that integration of tandem repeat copies of vector DNA was common. In addition, multiple integrations of the transforming vector at different chromosomal sites were also observed. The establishment of a... -
Message in a Bottle: Chemical Biology of Induced Disease Resistance in Plants
한국식물병리학회 The Plant Pathology Journal 24 Pages
한국식물병리학회 The Plant Pathology Journal 2008, 24권 3호 1 245-268 (24 pages)
and the known biological target(s) of each chemical. Larger polymeric structures and peptides/proteins are also discussed in this context. The quest for novel defence-inducing molecules would be aided by the capability for high-throughput analysis of candidate compounds, and we describe some issues associated with the development of these types of screens. Subsequent characterization of hits can be a formidable challenge, especially in terms of identifying chemical targets in plant cells. A... -
Candidatus Phytoplasma trifolii Associated with Witches` broom of Lespedeza cyrtobotrya M.
한국식물병리학회 The Plant Pathology Journal 3 Pages
한국식물병리학회 The Plant Pathology Journal 2007, 23권 2호 11 106-108 (3 pages)
It was most closely related to alsike clover proliferation phytoplasma (99.8% similarity, acce- ssion no. AY390261), Candidatus Pytoplasma trifolii strain. RFLP patterns generated with Alu I, Hpa II clearly differentiated LLWB phytoplasma from the referenced phytoplasma strains, water dropwort witches`broom, mulberry dwarf, glehni aster yellow dwarf and jujube witches` broom. This paper is the first report on Candidatus Phytoplasma trifolii in leafy lespedeza identified at a molecular level. -
A Large Genomic Deletion in Gibberella zeae Causes a Defect in the Productionof Two Polyketides but not in Sexual Development or Virulence
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2006, 22권 3호 3 215-221 (7 pages)
of a mutant (Z43R606) that produced neither aurofusarin nor zearalenone yet showed normal female fertility and virulence on host plants. Outcrossing analysis confirmed that both the albino and zearalenone-deficient mutations are linked to the insertional vector in Z43R606. Molecular characterization of Z43R606 revealed a deletion of at least 220 kb of the genome at the vector insertion site, including the gene clusters required for the biosynthesis of aurofusarin and zearalenone, respectively. A... -
Note : Occurrence of Bean common mosaic virus (BCMV) Infecting Peanut in Korea
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2006, 22권 1호 15 97-102 (6 pages)
Inclusion bodies such as scroll, pinwheel and long laminated aggregates induced by the virus in host plant cells were similar to those induced by members of the Potyvirus subdivision III. The virus showed < 95% homology with Bean common mosaic virus (BCMV), BCMV-BICMV/AzMV strains and only < 91% with Desmodium mosaic virus. Based on biological characterization, electron microscopy and molecular analyses of a Korean isolate (Daewon 1), the virus was identified as peanut stripe strain of BCMV. -
Virulence Differentiation of Eight Turnip mosaic virus Isolates Infecting Cruciferous Crops
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2005, 21권 4호 11 369-376 (8 pages)
representative eight isolates of TuMV were divided into two major groups/or six types. Group I includes Tu 1, Ca-ad7, and Cj-ca2-1 isolates, while group II includes the other isolates (rg-pf1, r 9-10, Rhcq1- 2, Stock and Mustard). According to the molecular phylogenetic analysis, these isolates, however, divided into two groups and two independent isolates. Phylogenetic analysis indicated that four isolates (Tu 1, r 9-0,Stock and Rh-cq1-2) formed a distinct phylogenetic group, and the other two...


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