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331.

A multiplex quantitative real-time polymerase chain reaction panel for detecting neurologic pathogens in dogs with meningoencephalitis

Jae-Ik Han, Dong-Woo Chang, Ki-Jeong Na 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 12 341-347 (7 pages)

Jae-Ik Han, Dong-Woo Chang, Ki-Jeong Na 저
...
- 3,000원
Meningoencephalitis (ME) is a common inflammatory disorder of the central nervous system in dogs. Clinically, ME has both infectious and non-infectious causes. In the present study, a multiplex quantitative real-time polymerase chain reaction (mqPCR) panel was optimized for the detection of eight canine neurologic pathogens (Blastomyces dermatitidis, Cryptococcus spp., Neospora caninum, Borrelia burgdorferi, Bartonella spp., Toxoplasma gondii, Ehrlichia canis, and canine distemper virus [CDV])....
332.

The potential molecular effects of bursal septpeptide II on immune induction and antitumor activity

Guang Fang Zhou, Qing Tao Liu, Bin Zhou, Ya Feng Qiu, Xiao Dong Liu, Zhi Yong Ma, Xiu Li Feng, Rui Bing Cao, Pu Yan Chen 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 10 325-331 (7 pages)

Guang Fang Zhou, Qing Tao Liu, Bin Zhou, Ya Feng Qiu, Xiao Dong Liu, Zhi Yong Ma, Xiu Li Feng, Rui Bing Cao, Pu Yan Chen 저
...
- 3,000원
The bursa of Fabricius (BF) is the acknowledged central humoral immune organ in birds. Bursal septpeptide II (BSP-II) is an immunomodulatory bioactive peptide isolated from BF. To understand the effects of BSP-II on immune induction, gene expression profiles of hybridoma cells treated with BSP-II were evaluated. Pathway analysis showed that regulated genes were involved in cytokine-cytokine receptor interactions, T cell receptor signaling pathway, and pathway in cancer. It was observed that...
333.

Establishment and evaluation of a murine ανβ3-integrin- expressing cell line with increased susceptibility to Foot- and-mouth disease virus

Wei Zhang, Kaiqi Lian, Fan Yang, Yang Yang, Zhijian Zhu, Zixiang Zhu, Weijun Cao, Ruoqing Mao, Ye Jin, Jijun He, Jianhong Guo, X 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 3 265-272 (8 pages)

Wei Zhang, Kaiqi Lian, Fan Yang, Yang Yang, Zhijian Zhu, Zixiang Zhu, Weijun Cao, Ruoqing Mao, Ye Jin, Jijun He, Jianhong Guo, X 저
...
- 3,000원
Integrin ανβ3 plays a major role in various signaling pathways, cell apoptosis, and tumor angiogenesis. To examine the functions and roles of ανβ3 integrin, a stable CHO-677 cell line expressing the murine ανβ3 heterodimer (designated as “CHO-677-mανβ3” cells) was established using a highly efficient lentiviral-mediated gene transfer technique. Integrin subunits αν and β3 were detected at the gene and protein levels by polymerase chain reaction (PCR) and indirect...
334.

Quantitative real-time polymerase chain reaction for detecting Mycoplasma hyosynoviae and Mycoplasma hyorhinis in pen-based oral, tonsillar, and nasal fluids 

João Carlos Gomes Neto, Leslie Bower, Barbara Z. Erickson, Chong Wang, Matthew Raymond, Erin L. Strait 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 2호 9 195-201 (7 pages)

João Carlos Gomes Neto, Leslie Bower, Barbara Z. Erickson, Chong Wang, Matthew Raymond, Erin L. Strait 저
...
- 3,000원
Mycoplasma (M.) hyorhinis and M. hyosynoviae are pathogens known to cause disease in pigs post-weaning. Due to their fastidious nature, there is increased need for culture-independent diagnostic platforms to detect these microorganisms. Therefore, this study was performed to develop and optimize quantitative real-time PCR (qPCR) assays to rapidly detect M. hyorhinis and M. hyosynoviae in pen-based oral fluids as well as nasal and tonsillar fluids as proxies for samples used in swine herd...
335.

The role of rpoS, hmp, and ssrAB in Salmonella enterica Gallinarum and evaluation of a triple-deletion mutant as a live vaccine candidate in Lohmann layer chickens

Youngjae Cho, Yoon Mee Park, Abhijit Kashinath Barate, So-Yeon Park, Hee Jeong Park, Mi Rae Lee, Quang Lam Truong, Jang Won Yoon 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 2호 8 187-194 (8 pages)

Youngjae Cho, Yoon Mee Park, Abhijit Kashinath Barate, So-Yeon Park, Hee Jeong Park, Mi Rae Lee, Quang Lam Truong, Jang Won Yoon 저
...
- 3,000원
Salmonella enterica Gallinarum (SG) causes fowl typhoid (FT), a septicemic disease in avian species. We constructed deletion mutants lacking the stress sigma factor RpoS, the nitric oxide (NO)-detoxifying flavohemoglobin Hmp, and the SsrA/SsrB regulator to confirm the functions of these factors in SG. All gene products were fully functional in wild-type (WT) SG whereas mutants harboring single mutations or a combination of rpoS, hmp, and ssrAB mutations showed hypersusceptibility to H2O2, loss...
336.

Cloning, expression and functional analysis of the duck Toll-like receptor 5 (TLR5) gene

Yuqiang Cheng, Yingjie Sun, Hengan Wang, Shuduan Shi, Yaxian Yan, Jing Li, Chan Ding, Jianhe Sun 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 1호 6 37-46 (10 pages)

Yuqiang Cheng, Yingjie Sun, Hengan Wang, Shuduan Shi, Yaxian Yan, Jing Li, Chan Ding, Jianhe Sun 저
...
- 3,000원
Toll-like receptor 5 (TLR5) is responsible for the recognition of bacterial flagellin in vertebrates. In the present study, the first TLR5 gene in duck was cloned. The open reading frame (ORF) of duck TLR5 (dTLR5) cDNA is 2580 bp and encodes a polypeptide of 859 amino acids. We also cloned partial sequences of myeloid differentiation factor 88, 2’-5’-oligoadenylate synthetase (OAS), and myxovirus resistance (Mx) genes from duck. dTLR5 mRNA was highly expressed in the...
337.

Use of laparoscopy for diagnosing experimentally induced acute pancreatitis in dogs

Hyun-wook Kim, Ye-in Oh, Ji-hye Choi, Dae-yong Kim, Hwa-young Youn, 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 16 551-556 (6 pages)

Hyun-wook Kim, Ye-in Oh, Ji-hye Choi, Dae-yong Kim, Hwa-young Youn, 저
...
- 3,000원
Diagnosis of acute pancreatitis in dogs remains a significant challenge despite the development of advanced diagnostic methodologies. Visual inspection and pancreas biopsy using laparoscopy are generally considered to be procedures free of complications when conducted on healthy animals. However, the usefulness of laparoscopy for diagnosing acute pancreatitis has not been assessed. In the present study, the efficacy of laparoscopy for diagnosing acute pancreatitis in dogs was evaluated in...
338.

Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage

Li-ming Wu, Rui Guo, Lin Hui, Yong-gang Ye, Jing-mei Xiang, Chun-yun Wan, Miao Zou, Rui Ma, Xiao-zhuan Sun, Shi-jin Yang, Ding-z 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 3 475-483 (9 pages)

Li-ming Wu, Rui Guo, Lin Hui, Yong-gang Ye, Jing-mei Xiang, Chun-yun Wan, Miao Zou, Rui Ma, Xiao-zhuan Sun, Shi-jin Yang, Ding-z 저
...
- 3,000원
recombinant plasmid for 48 h to over-express STC-1. Acridine orange/ ethidium bromide (AO/EB) double staining and trypan blue exclusion assays were then performed to measure cell viability and apoptosis of the cells, respectively. The expression of STC-1 and apoptosis-related proteins in the cells was monitored by real-time PCR and Western blotting. The results indicated that both STC-1 mRNA and protein expression levels positively correlated with the duration of H2O2 treatment. H2O2 damaged the...
339.

Enhancing mucosal immunity in mice by recombinant adenovirus expressing major epitopes of porcine circovirus-2 capsid protein delivered with cytosine-phosphate-guanosine oligodeoxynucleotides

Hong-tao Chang, Xiu-yuan He, Yu-Feng Liu, Lu Chen*, Quan-hai Guo, Qiu-ying Yu, Jun Zhao, Xin-wei Wang, Xia Yang, Chuan-qing Wang 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 8 399-407 (9 pages)

Hong-tao Chang, Xiu-yuan He, Yu-Feng Liu, Lu Chen*, Quan-hai Guo, Qiu-ying Yu, Jun Zhao, Xin-wei Wang, Xia Yang, Chuan-qing Wang 저
...
- 3,000원
A recombinant replication-defective adenovirus expressing the major epitopes of porcine circovirus-2 (PCV-2) capsid protein (rAd/Cap/518) was previously constructed and shown to induce mucosal immunity in mice following intranasal delivery. In the present study, immune responses induced by intranasal immunization with a combination of rAd/Cap/518 and cytosine-phosphate-guanosine oligodeoxynucleotides (CpG ODN) were evaluated in mice. The levels of PCV-2-specific IgG in serum and IgA in saliva,...
340.

Molecular characterization of duck enteritis virus CHv strain UL49.5 protein and its colocalization with glycoprotein M

Meng Lin, Renyong Jia*, Mingshu Wang, Xinghong Gao, Dekang Zhu, Shun Chen, Mafeng Liu, Zhongqiong Yin, Yin Wang, Xiaoyue Chen, A 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 7 389-398 (10 pages)

Meng Lin, Renyong Jia*, Mingshu Wang, Xinghong Gao, Dekang Zhu, Shun Chen, Mafeng Liu, Zhongqiong Yin, Yin Wang, Xiaoyue Chen, A 저
...
- 3,000원
to molecular characterization. To verify the predicted intracellular localization of gene expression, the recombinant plasmid pEGFP-C1/pUL49.5 was constructed and used to transfect duck embryo fibroblasts. Next, the recombinant plasmid pDsRed1-N1/ glycoprotein M (gM) was produced and used for co-transfection with the pEGFP-C1/pUL49.5 plasmid to determine whether DEV pUL49.5 and gM (a conserved protein in herpesviruses) colocalize. DEV pUL49.5 was thought to be an envelope glycoprotein with a...

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