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Effects of cGMP on the Contractility and Ca Movement in the Aorta of Normotensive Wistar-Kyoto Rats and Spontaneously Hypertensive Rats
Park, Hae-Kun, Jeon, Byeong-Hwa, Kim, Se-Hoon, Kim, Hoe-Suk, Chang, Seok-Jong 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1994, 제 28권 제 2호 8 181-190 (10 pages)
Endothelium-derived relaxing factor (EDRF) activates guanylate cyclase which mediates the formation of cGMP from GTP in vascular smooth muscle. It is well known that endothelium-dependent relaxation is impaired in spontaneously hypertensive rats (SHR). However, it is still unknown whether the impaired endothelium-dependent relaxation in SHR results from the reduced release of EDRF or from the decrease of vascular response to EDRF. We investigated the effects of cGMP on the contractility and Ca... -
Effect of pH on the Vascular Tone and 45Ca Uptake in the Aorta of Spontaneously Hypertensive Rats
Chang, Seok-Jong, Jeon, Byeong-Hwa, Kim, Se-Hoon, Kim, Hoe-Suk, Park, Hae-Kun 대한생리학회 대한생리학회지 12 Pages
대한생리학회 대한생리학회지 1994, 제 28권 제 2호 7 169-180 (12 pages)
The effect of extracellular and intracellular pH on vascular tone and 45Ca uptake were investigated in aortic strips and dispersed single aortic smooth muscle cells of spontaneously hypertensive rats (SHR) and aged-matched Wistar-Kyoto rats (WKY). The contraction produced by a change of extracellular pH (pHo) in the range of 6.5 ~ 8.3 was estimated by comparison with the level of vascular tone at pH 7.4. Contraction was induced below pHo 6.5 in WKY, pHo 7.1 in SHR, and over pHo 8.0 on both... -
Role of Na+Ca2+ Exchange in the Control of Contractility in Rabbit Basilar Arterial Smooth Muscle
Kim Eui-Yong, Han Jin 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1994, 제 28권 제 2호 6 159-168 (10 pages)
intracellular Ca2+ release from the sarcoplasmic reticulum, did not suppress the development of Na+-free contracture. The contractile response to norepinephrine (10-6 M) was augmented by reducing the extracellular Na+ concentration. The relaxation rate from caffeine-induced contraction was dependent on the extracellular Na+ concentration (0 mM, 140 mM). From the above results, it could be suggested that Na+/Ca2+ exchange can move Ca2+ either into or out of rabbit basilar arterial smooth muscle.... -
Effect of Cyclic GMP on the Calcium Current in Rabbit Ventricular Myocytes
Han, Jin, Leem, Chae-Hun, Ahn, Chul-Soo, So, In-Suk, Kim, Eui-Yong, Ho, Won-Kyung, Earm, Yung-E 대한생리학회 대한생리학회지 12 Pages
대한생리학회 대한생리학회지 1993, 제 27권 제 2호 4 151-162 (12 pages)
In order to investigate the effect of intracellular cyclic GMP on calcium current the whole-cell patch clamp technique with internal perfusion method was used in isolated ventricular myocytes of the rabbit. Cyclic GMP, 8-bromo-cyclic GMP, cyclic AMP, isoprenaline and forskolin were perfused into cells and their effects on calcium current were analysed by applying depolarizing step pulses of + 10 mV in amplitude far 300 msec from holding potential of - 40 mV. Not only cyclic AMP (100 μM) but... -
Acute and Chronic Effects of Ethanol on the Cardiovascular and Hormonal Responses to Hemorrhage in Conscious Normotensive and Spontaneously Hypertensive Rats
Park, Yoon-Yub, Lee, Joong-Hee, Park, Jae-Sik, Yang, Eun-Kyoung, Ahn, Dong-Kuk, Kim, Hyeong-Jin, Lee, Won-Jung, 대한생리학회 대한생리학회지 12 Pages
대한생리학회 대한생리학회지 1993, 제 27권 제 1호 7 67-78 (12 pages)
Acute and chronic effects of ethanol (EOH) administration on the cardiovascular and hormonal responses to repeated hemorrhage were investigated in conscious normotensive Wistar rats and spontaneously hypertensive rats (SHR). The chronic EOH treated group received 5% EOH (vol/vol) ad libitum in the drinking water far the first week,10% for the last 2 weeks, and 20% for the last 5 weeks from the age of 6 weeks. The EOH free group received tap water. Chronic EOH and EOH free groups were randomly... -
Effects of the Endothelium on the Contractile Responses to Norepinephrine in Isolated Proximal and Distal Coronary Artery of Pigs
Kim, Jong-Hoon, Jeon, Byeong-Hwa, Chang, Seok-Jong, Park, Hae-Kun 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1993, 제 27권 제 1호 3 27-36 (10 pages)
Effects of the endothelium on the contractile responses to norepinephrine (NE) were investigated in isolated helical strips of the proximal and distal coronaries artery of pigs. The helical strips were immersed in Tris-buffered Tyrode s solution equilibrated with 100% O2 at 35℃ and its isometric tension was measured. NE relaxed the strips precontracted with acetylcholine from both the proximal and distal coronary artery. NE-induced relaxation, which might be induced mainly by β-adrenoceptor... -
Calcium Current and Background Current Activation in L-triiodothyronine Loaded Ventricular Myocytes of the Rabbit
Han, Jin, Kim, Eui-Yong, Han, Jae-Hee, Park, Choon-Ok, Hong, Seong-Geun, Leem, Chae-Hun, So, In-Suk, Ho, Won-Kyung, Earm, Yung-E, Sung, H 대한생리학회 대한생리학회지 14 Pages
대한생리학회 대한생리학회지 1992, 제 26권 제 2호 1 99-112 (14 pages)
change in myocardial contractile state and T3-induced arrhythmias were investigated in rabbit ventricular or atrial myocytes using whole cell patch clamp technique. Single cells were isolated by Langendorff perfusion with collagenase. Cardiac myocytes were incubated in low-Cl-,, high-K+ medium containing 1μM L-triiodothyronine (T3) at 4℃ for 2.10 hours. The calcium currrent (ICa) was increased in T3 loaded cells, however, the shape of current voltage curve and reverse potential did not... -
A Study on the Na+/Ca2+ Exchange Mechanism in the Smooth Muscle of Guinea-pig Stomach
Kim, Eui-Yong, Han, Jin, Kim, Ki-Whan 대한생리학회 대한생리학회지 14 Pages
대한생리학회 대한생리학회지 1992, 제 26권 제 1호 6 55-68 (14 pages)
blocking the influence of intrinsic nerves by the pretreatment with atropine, guanethidine and TTX. 4. Ca2+-channel blockers(D-600 or Mn2+) and the blocker of intracellular Ca2+ release from sarcoplasmic reticulum(ryanodine) did not suppress the development of Na+-free contracture. And also, dinitrophenol had no effect on Na+-free contracture. 5. Dose-response relationship between extracellular Na+ concentrations and the magnitude of contractures showed a sigmoid pattern. The slope of straight... -
Effects of pH, PCO2, and Adenosine on the Contractility of Pig Coronary Artery
Chang, Seok-Jong, Kim, Il-Seon, Jeon, Byeong-Hwa, Kim, Se-Hoon 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1992, 제 26권 제 1호 5 45-54 (10 pages)
Effects of pH, PCO2, and adenosine on the vascular contractility were investigated in the pig coronary arteries. The helical strips of isolated coronary arteries were immersed in the HEPES or HCO3-/CO2-buffered Tyrode s solution equilibrated with 100% O2 or 95% O2-5% CO2 at 35℃. The contraction was recorded isometrically using a force transducer. The amplitudes of contraction induced by ACh, high K+, and electrical Held stimulation (EFS) were decreased by elevating extracellular pH (pHo) and... -
Ionic Dependence and Modulatory Factors of the Background Current Activated by Isoprenaline in Rabbit Ventricular Cells
Leem, Chae-Hun, Lee, Suk-Ho, So, In-Suk, Ho, Won-Kyung, Earm, Yung-E 대한생리학회 대한생리학회지 12 Pages
대한생리학회 대한생리학회지 1992, 제 26권 제 1호 2 15-26 (12 pages)
In order to elucidate the properties of the background current whole cell patch clamp studies were performed in rabbit ventricular cells. Ramp pulses of ±80 mV from holding potential of 40 mV(or 20 mV) at the speed of 0.8 V/sec were given every 30 sec(or 10 sec) and current-voltage diagrams(I-V curve) were obtained. For the activation of the background current isoprenaline, adenosine 3 ,5 -cyclic monophosphate(dBcAMP), guanosine 3 ,5 -cyclic monophosphate(cGMP), and N6-2 -o-dibutyryladenosine 3...
