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Biological characteristics of Chinese hamster ovary cells transfected with bovine Prnp
Sang-Gyun Kang, Deog-Yong Lee, Mi Lan Kang, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 5 131-137 (7 pages)
A normal prion protein (PrPc) is converted to a proteaseresistant isoform by an apparent self-propagating activity in transmissible spongiform encephalopathy, a neurodegenerative disease. The cDNA encoding open reading frame (ORF) of the bovine prion protein gene (Prnp) was cloned from Korean cattle by PCR, and was transfected into Chinese hamster ovary (CHO-K1) cells using lipofectamine. The gene expression of the cloned cDNA was confirmed by RT-PCR and Western blotting with the monoclonal... -
Radioprotective effects of an acidic polysaccharide of Panax ginseng on bone marrow cells
Hyun-Ji Kim, Mi-Hyoung Kim, Yun-Young Byon, Jae Woo Park, Youngheun Jee, Hong-Gu Joo 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 6 39-44 (6 pages)
An acidic polysaccharide of Panax ginseng (APG), so called ginsan is known to have important immunomodulatory activities. It was recently reported that APG has radioprotective effects in mice but the detailed mechanism was not fully elucidated. This study examined the effects of APG on bone marrow cells (BMs). The phenotypical and functional changes in APG-treated BMs after gamma radiation were studied. The benefit of APG on BMs damaged by gamma radiation was determined by measuring the cell... -
BSE situation and establishment of Food Safety Commission in Japan
Takashi Onodera, Chi-Kyeong Kim 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 1 1-11 (11 pages)
Eight major policies were implemented by Japanese Government since Oct.2001, to deal with bovine spongiform encephalopathy (BSE).These are;1)Surveillance in farm by veterinarian, 2)Prion test at healthy 1.3mi cows/yr, by veterinarian, 3)Elimination of specified risk material (SRM), 4)Ban of MBM for production, sale use, 5)Prion test for fallen stocks, 6)Transparent information and traceability, 7)New Measures such as Food Safety Basic Law, and 8)Establish of Food Safety Commission in the Cabinet... -
A predictive model for the level of sIgA based on IgG levels following the oral administration of antigens expressed in Sacchromyces cerevisiae
Sung Jae Shin, Seung Won Shin, Eun Jin Choi, Deog Yong Lee, Jeong Min Ahn, Moon Sik Yang, Yong Suk Jang, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 4호 8 305-309 (5 pages)
Oral vaccination may be the most efficient way of inducing an immune response at the remote mucosal site through the common mucosal immune network. Antigenspecific secretory IgA (sIgA) is the major immunoglobulin type generally detected in the secretions of experimental animals following an effective oral immunization. Actinobacillus pleuropneumoniae causing disease in the lung of pig initially interacts, colonizes, and infects the host tissues at the mucosal surface of the respiratory tract.... -
Expression of tyrosine kinase A in the cerebral cortex of postnatal developing rat
Hyo Jung Kwon, Kyoung Youl Lee, Il Kwon Park, Mi Sun Park, Mi Young Lee, Moo Kang Kim 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 3호 2 185-189 (5 pages)
all layers. These TrkA-positive cells were not detected at PD0. In contrast, there was significant increase in the percentage of cells exhibiting TrkA-IR with development and the highest level was detected at PD15. These results suggest that the cerebral cortex expresses TrkA strongly during the postnatal period. Moreover, the postnatal development-related increase in the expression of TrkA-cells shows that NGF may have a trophic effect on these cerebral cortex neurons from the postnatal period. -
Mitral valve prolapse in Cavalier King Charles Spaniel:A review and case study
Changbaig Hyun 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 1호 10 67-73 (7 pages)
A 5 year-old spayed female Cavalier King Charles Spaniel was presented after a 3- to 5-day onset of severe respiratory distress. The dog also had a history of several episodes of syncope prior to presentation. A comprehensive diagnostic investigation revealed a midsystolic click sound on cardiac auscultation, signs of left sided cardiac enlargement in ECG and thoracic radiography, mitral valvular leaflet protrusion into left the atrium, decreased E-point-to septal separation (EPSS) and mitral... -
Immunization of mice with recombinant P27/30 protein confers protection against hard tick Haemaphysalis longicornis (Acari: Ixodidae) infestation
Myung-Jo You 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 1호 7 47-51 (5 pages)
The success of immunological control methods is dependent upon the use of potential key antigens as tick vaccine candidates. Previously, we cloned a gene encoding 27 kDa and 30 kDa proteins (P27/30) of Haemaphysalis longicornis, and identified the P27/30 is a troponin I-like protein. In this study, the recombinant P27/30 (rP27/30) expressed in Escherichia coli was used to immunize mice and the mice were challenge-infested with ticks at different developmental stages of the same species. The... -
Distribution of trkA in cerebral cortex and diencephalon of the mongolian gerbil after birth
Il-Kwon Park, Xilin Hou, Kyung-Youl Lee, O-Sung Park, Kang-Yi Lee, Min-Young Kim, Tae-Sun Min, Geun-Jwa Lee, Won-Sik Kim, Moo-Kan 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 4 303-307 (5 pages)
TrkA is essential components of the high-affinity NGF receptor necessary to mediate biological effects of the neurotrophins NGF. Here we report on the expression of trkA in the cerebral cortex and diencephalon of mongolian gerbils during postnatal development. The expression of trkA was identified by immunohistochemical method. In parietal cortex and piriform cortex, higher levels of trkA-IR (immunoreactivity) were detected at 3 days postnatal (P3) and at P9. Although trkA was not expressed till... -
A hematogenic pleuropneumonia caused by postoperative septic thrombophlebitis in a Thoroughbred gelding
Seung-ho Ryu, Joon-gyu Kim, Ung-bok Bak, Chang-woo Lee and Yonghoon Lyon Lee 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 1호 11 75-77 (3 pages)
A 7-year-old Thoroughbred gelding was admitted to Equine Hospital, Korea Racing Association for evaluation and treatment of colic. Based on the size and duration of the large colonic and cecal impaction, a routine ventral midline celiotomy and large colon enterotomy were performed to relieve the impaction. Six days following surgery the gelding exhibited signs of lethargy, fever, inappetence and diarrhea. Eleven days following surgery, the jugular veins showed a marked thrombophlebitis. On the... -
Altered maturation of dendritic cells by taxol, an anticancer drug
Hong-Gu Joo 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 3호 5 229-234 (6 pages)
Taxol is a clinically useful anticancer drug against a variety of cancers. Although it has been known that taxol induces the apoptosis of cancer cells through cytochrome C release and the activation of caspases, the effect of taxol on dendritic cells (DCs) has not been studied. In this study, taxol enhanced the expression of MHC class II on DCs, compared to medium-treated immature DCs. Surprisingly, the viability of DCs was not decreased by taxol, whereas that of cancer cells was. It was...


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