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Effects of cGMP on the Contractility and Ca Movement in the Aorta of Normotensive Wistar-Kyoto Rats and Spontaneously Hypertensive Rats
Park, Hae-Kun, Jeon, Byeong-Hwa, Kim, Se-Hoon, Kim, Hoe-Suk, Chang, Seok-Jong 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1994, 제 28권 제 2호 8 181-190 (10 pages)
by the pretreatment with 8-Br-cGMP (10-4 M) in either strain. However, the amplitudes of suppression induced by 8-Br-cGMP were greater in SHR than that in WKY. Basal 45Ca uptake and 40mM K+-stimulated 45Ca uptake were not suppressed by pretreatment with 8-Br-cGMP (10-4 M) in single aortic smooth muscle cells of both SHR and WKY. From the above results, it is suggested that cGMP decreases Ca sensitivity in vascular smooth muscle cells and that the impaired endothelium-dependent relaxation in the... -
The Excitatory Mechanism of Substance P in the Antral Circular Muscle of Guinea Pig Stomach
Jun, Jae-Yeoul, Kim, Sung-Joon, Choi, Youn-Baik, Kim, Ki-Whan 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1994, 제 28권 제 1호 5 51-60 (10 pages)
This study was carried out to elucidate the excitatory mechanisms of Substance P in the antral circular muscle, using isometric contraction recording, conventional microelectrode method and whole-cell patch clamp technique. Substance P produced tonic and phasic contractions in a dose-dependent manner and depolarized membrane potential with increased amplitude of slow waves in muscle strips. Voltage-dependent Ca2+ currents were increased by the application of Substance P from a holding potential... -
Role of Na+-K+ Pump on Endothelium-dependent Relaxation
Sung, Sang-Hyun, Roh, Joon-Ryang, Park, Tae-Sic, Suh, Suk-Hyo, Hwang, Sang-Ik, Kim, Ki-Whan 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1993, 제 27권 제 2호 8 199-208 (10 pages)
To study the underlying mechanism through which the endothelium-dependent relaxation is inhibited by blocking the Na+ -K+ pump, the effects of Na+ -K+ pump blockade on the release of EDRF and its relaxing activity were examined, using organ bath study, bioassay technique, and cGMP measurement. Endothelium-dependent relaxation was attenuated by blocking the Na+ -K+ pump in the vascular ring with intact endothelium. In bioassay experiment EDRF release was decreased with the blockade of the Na+... -
A Study on the Na+/Ca2+ Exchange Mechanism in the Smooth Muscle of Guinea-pig Stomach
Kim, Eui-Yong, Han, Jin, Kim, Ki-Whan 대한생리학회 대한생리학회지 14 Pages
대한생리학회 대한생리학회지 1992, 제 26권 제 1호 6 55-68 (14 pages)
contracture was developed even after blocking the influence of intrinsic nerves by the pretreatment with atropine, guanethidine and TTX. 4. Ca2+-channel blockers(D-600 or Mn2+) and the blocker of intracellular Ca2+ release from sarcoplasmic reticulum(ryanodine) did not suppress the development of Na+-free contracture. And also, dinitrophenol had no effect on Na+-free contracture. 5. Dose-response relationship between extracellular Na+ concentrations and the magnitude of contractures showed a... -
A Study on Na+ and Water Reabsorption in the Nephron Segment Beyond Proximal Tubule Measured by Lithium Clearance
Han, O-Soo, Goo, Yong-Sook, Sung, Ho-Kyung 대한생리학회 대한생리학회지 12 Pages
대한생리학회 대한생리학회지 1991, 제 25권 제 2호 9 189-200 (12 pages)
During the past few years it has been proposed that lithium clearance can be used as a reliable measure for the outflow of tubular fluid from the proximal tubule. This study was aimed to characterize the inflow dependent reabsorption of Na in renal tubule beyond the proximal tubule. For this purpose, lithium clearance was used as a measure for the inflow from the proximal tubule and the changes in reabsorption fraction of Na and water were determined in rabbits. Rabbits were pretreated with... -
Inhibition of Dicarboxylate Transport by p-chloromercuribenzoic Acid (PCMB) in Plasma Membrane Vesicles of Rabbit Proximal Tubule
Kim, Yong-Keun, Kim, Tae-In, Jung, Jin-Sup, Lee, Sang-Ho 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1991, 제 25권 제 2호 8 179-188 (10 pages)
by a pretreatment of vesicles with dithiothreitol. PCMB did not increase Na+ permeability at concentrations inhibiting succinate uptake. The PCMB inhibition of succinate uptake was due to a change in Vmax, but not in Km. When membrane vesicles were pretreated with PCMB in the presence of unlabelled succinate, the inhibitory effect was significantly reduced. In both BBMV and BLMV, succinate uptake was inhibited by various sulfhydryl reagents with the inhibitory potency of following order:... -
Mechanisms of Contraction Induced by Sodium Depletion in the Rabbit Renal Artery
Kim, Se-Hoon, Chang, Seok-Jong, 대한생리학회 대한생리학회지 12 Pages
대한생리학회 대한생리학회지 1991, 제 25권 제 2호 6 159-170 (12 pages)
on the nature of the Na substitutes. Na-free solution (choline) produced the contraction in ouabain-pretreated artery (Na loaded artery) even in the presence of verapamil. The amplitude of the contraction was dependent on the duration of the pretreatment with ouabain (105 M). Monensin potentiated the effect of ouabain on the contraction. Removal of Ca from bathing solution abolished the contraction and the substitution of Sr for Ca produced the contraction. Divalent cations such as Mg, Mn... -
The Contractile and Electrical Responses of Guinea-pig s Gastric Smooth Muscle to Serotonin
Lee, Sang-Jin, Hwang, Sang-Ik, Kim, Ki-Whan 대한생리학회 대한생리학회지 14 Pages
대한생리학회 대한생리학회지 1991, 제 25권 제 2호 4 133-146 (14 pages)
using various methods including isometric contraction recording, transmural electrical field stimulation, junction potential recording, intracellular microelectrode technique, and partition stimulation method. The results were as follows: 1) The effect of serotonin on spontaneous contractions was inhibitory in the circular muscle strips of the antrum and fundus, while it was excitatory in the longitudinal muscle strips of the antrum and fundus. Serotonin changed mainly phasic contractions of... -
토끼 대동맥 평활근의 내피세포 의존성 이완에 미치는 Ca2+ 및 Ca2+ 길항제의 효과
서석효(Suh, Suk-Hyo), 구용숙(Goo, Yong-Sook), 박춘옥(Park, Choon-Ok), 황상익(Hwang, Sang-Ik), 김기환(Kim, Ki-Whan) 대한생리학회 대한생리학회지 12 Pages
대한생리학회 대한생리학회지 1990, 제 24권 제 1호 8 91-102 (12 pages)
토끼 흉부 대동맥을 이용하여 내피세포 의존성 혈관이완에 대한 세포외 Ca2+과 여러가지 Ca2+ 길항제의 효과를 분석하여 EDRF의 작용기전을 밝혀 보고자 하였다. 대동맥 횡단 절편의 등장성 수축은 10-7 M 노에피네프린으로 유발시켰으며, 10-6 M 사세틸콜린으로 내피세포 의존성 혈관이완을 일으켰다. 내피세포는 작은 솜뭉치로 부드럽게 문질러서 제거하였으며, hemolysate를 사용하여 EDRF에 대한 헤모글로빈의 효과를 관찰하였다. 결과를 종합하면 다음과 같다. 1) 아세틸콜린에 의한 내피세포 의존성 혈관이완은 두 시기, 즉...


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