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361.

Molecular heterogeneity of plpE gene in Indian isolates of Pasteurella multocida and expression of recombinant PlpE in vaccine strain of P. multocida serotype B: 2

Ajay Pratap Singh, Satparkash Singh, Rajeev Ranjan*, Santosh Kumar Gupta, Vijendra Pal Singh, Bhaskar Sharma 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 8 227-233 (7 pages)

Ajay Pratap Singh, Satparkash Singh, Rajeev Ranjan*, Santosh Kumar Gupta, Vijendra Pal Singh, Bhaskar Sharma 저
...
- 3,000원
Outer membrane proteins of Pasteurella (P.) multocida have been known to be protective immunogens. Pasteurella lipoprotein E (PlpE) has been reported to be an important cross reactive outer membrane protein in P. multocida. The gene encoding the PlpE of P. multocida serotypes A: 3, B: 2 and D: 1 was amplified from the genomic DNA. The amplified products were cloned and the nucleotide sequence was determined. Sequence analysis of the recombinant clones revealed a single open reading frame of...
362.

Inhibitory effects of interleukin-10 plasmid DNA on the development of atopic dermatitis-like skin lesions in NC/Nga mice

Bock-Gie Jung, Sun-Ju Cho, Jae-Hyung Ko, Bong-Joo Lee* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 6 213-220 (8 pages)

Bock-Gie Jung, Sun-Ju Cho, Jae-Hyung Ko, Bong-Joo Lee* 저
...
- 3,000원
eosinophil ratio and serum IgE concentration, and a reduction in the number of Staphylococcus aureus recovered from the ear. Moreover, relative mRNA expression levels of IL-4 and interferon-g in the skin lesions of mice injected with IL-10 plasmid DNA were also decreased compared with those of control mice. Of note, higher serum IL-10 levels in mice injected with IL-10 plasmid DNA were maintained compared with those in control mice. Taken together, the results indicate that IL-10 plasmid DNA can...
363.

Antigenic and immunogenic investigation of the virulence motif of the Newcastle disease virus fusion protein

Kang-Seuk Choi*, Eun-Kyoung Lee, Woo-Jin Jeon, Jun-Hun Kwon 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 5 205-211 (7 pages)

Kang-Seuk Choi*, Eun-Kyoung Lee, Woo-Jin Jeon, Jun-Hun Kwon 저
...
- 3,000원
Newcastle disease (ND) caused by virulent Newcastle disease virus (NDV) is a highly contagious viral disease of poultry. Virulent NDVs characteristically have a multibasic amino acid sequence (virulence motif) such as RRQKRF at the cleavage site of the precusor fusion (F0) protein. The antigenic and immunogenic characteristics of the virulence motif RRQKRF in the F0 protein of virulent NDVs were investigated. Epitope mapping analysis revealed that a RRQKRF-specific monoclonal antibody 4G2...
364.

Autologous conditioned plasma as therapy of tendon and ligament lesions in seven horses

Rindermann Georg, Cislakova Maria, Arndt Gisela, Carstanjen Bianca* 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 14 173-175 (3 pages)

Rindermann Georg, Cislakova Maria, Arndt Gisela, Carstanjen Bianca* 저
...
- 1,500원
This case report describes the intralesional application of autologous conditioned plasma (ACP) in seven horses as treatment of severe tendinitis of the superficial digital flexor tendon, deep digital flexor tendon, or desmitis of the inferior check ligament. Follow-up data of the horses revealed a positive outcome in 10 to 13 months post injury. All horses treated with ACP were either performing in their previous work-load or were back in full training. Further studies with long-term follow-up...
365.

Relationship between sperm quality traits and field-fertility of porcine semen

I. A. Tsakmakidis, A. G. Lymberopoulos, T. A. A. Khalifa 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 9 151-154 (4 pages)

I. A. Tsakmakidis, A. G. Lymberopoulos, T. A. A. Khalifa 저
...
- 1,500원
for 6 months. The semen samples were evaluated for subjective sperm motility and concentration. Ejaculates with at least 1 × 10 sperm/mL and 70% sperm progressive motility were extended with a commercial medium to 30 × 10 sperm/mL and used for artificial insemination (AI). AI dose was 100 mL semen containing 3 × 10 spermatozoa. Aliquots of diluted semen were assessed for live morphologically normal spermatozoa (LMNS, eosin-nigrosin stain exclusion assay) and sperm chromatin instability (SCI,...
366.

Evaluation of infectivity and transmission of different Asian foot-and-mouth disease viruses in swine

Juan M. Pacheco, P. W. Mason 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 7 133-142 (10 pages)

Juan M. Pacheco, P. W. Mason 저
...
- 3,000원
Most isolates of foot-and-mouth disease virus (FMDV) display a broad host range. Since the late 1990s, the genetic lineage of PanAsia topotype FMDV serotype O has caused epidemics in the Far East, Africa, the United Kingdom, France, the Netherlands, and numerous other countries throughout Europe and Asia. In contrast, there are several FMDV isolates that exhibit a more restricted host range. A Cathay topotype isolate of FMDV serotype O from the 1997 epizootic in Taiwan (O/TAW/97) demonstrated...
367.

spa typing and enterotoxin gene profile of Staphylococcus aureus isolated from bovine raw milk in Korea

Sun Young Hwang, Young Kyung Park, Hye Cheong Koo, Yong Ho Park 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 6 125-131 (7 pages)

Sun Young Hwang, Young Kyung Park, Hye Cheong Koo, Yong Ho Park 저
...
- 3,000원
Staphylococcus aureus is a major etiological pathogen of bovine mastitis, which triggers significant economic losses in dairy herds worldwide. In this study, S. aureus strains isolated from the milk of cows suffering from mastitis in Korea were investigated by spa typing and staphylococcal enterotoxin (SE) gene profiling. Forty-four S. aureus strains were isolated from 26 farms in five provinces. All isolates grouped into five clusters and two singletons based on 14 spa types. Cluster 1 and 2...
368.

Involvement of the Fas and Fas ligand in testicular germ cell apoptosis by zearalenone in rat

Youngheun Jee, Eun-Mi Noh, Eun-Sang Cho, Hwa-Young Son 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 4 115-119 (5 pages)

Youngheun Jee, Eun-Mi Noh, Eun-Sang Cho, Hwa-Young Son 저
...
- 1,500원
Zearalenone (ZEA), a nonsteroidal estrogenic mycotoxin, is known to cause testicular toxicity in animals. In the present study, the effects of ZEA on spermatogenesis and possible mechanisms involved in germ cell injury were examined in rats. Ten-week-old Sprague-Dawley rats were treated with 5 mg/kg i.p. of ZEA and euthanized 3, 6, 12, 24 or 48 h after treatment. Histopathologically, spermatogonia and spermatocytes were found to be affected selectively. They were TUNEL-positive and found to be...
369.

Induction of apoptotic lesions in liver and lymphoid tissues and modulation of cytokine mRNA expression by acute exposure to deoxynivalenol in piglets

Osamu Mikami, Hiroyuki Yamaguchi, Hideo Murata, Yasuyuki Nakajima, Shigeru Miyazaki 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 3 107-113 (7 pages)

Osamu Mikami, Hiroyuki Yamaguchi, Hideo Murata, Yasuyuki Nakajima, Shigeru Miyazaki 저
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- 3,000원
at the concentration of 1 mg/kg body weight, with three pigs each necropsied at 6 and 24 h post-injection (PI) for investigation of hepatotoxicity and immunotoxicity with special attention to apoptotic changes and cytokine mRNA expression. Histopathological examination of the DON-injected pigs revealed systemic apoptosis of lymphocytes in lymphoid tissues and hepatocytes. Apoptosis of lymphocytes and hepatocytes was confirmed by the TdT-mediated dUTP-biotin nick end-labeling (TUNEL) method and...
370.

Role of PKG-L-type calcium channels in the antinociceptive effect of intrathecal sildenafil

Woong Mo Kim, Myung Ha Yoon, Jin Hua Cui 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 2 103-106 (4 pages)

Woong Mo Kim, Myung Ha Yoon, Jin Hua Cui 저
...
- 1,500원
Sildenafil increases the cyclic guanosine monophosphate (cGMP) by inhibition of a phosphodiesterase 5, thereby leading to an antinociceptive effect. The increased cGMP may exert the effect on an L-type calcium channel through the activation of protein kinase G (PKG). The purpose of this study was to examine the possible involvement of a PKG-L- type calcium channel on the effect of sildenafil at the spinal level. Catheters were inserted into the intrathecal space of male SD rats. Pain was induced...

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